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ah woe is me

ah woe is me

ah woe is me

... boardingschool. They come home once a year at Christmas, and the first time thenarrator meets the children, she is surprised at their well-manneredbehaviour. She finds, however, that Sarah is a bit harsh ... this time. While she allows Sarah's children to stay in herhouse during Christmas, I think the only reason she does it is becauseshe tries to escape her own bad conscience. It is Christmas ... daughter comes to the house. Slowly she tells her story to thenarrator. How the younger brother is working now, and how she is takingcare of Sarah. The narrator offers her some clothes and some moneyand...
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Tài liệu Báo cáo khoa học: Phosphorylation of cyclin dependent kinase 4 on tyrosine 17 is mediated by Src family kinases pptx

Tài liệu Báo cáo khoa học: Phosphorylation of cyclin dependent kinase 4 on tyrosine 17 is mediated by Src family kinases pptx

... phosphory-lation is independent of p27KIP1.The finding that Y17 phosphorylation is mediatedby Src family kinases is completely novel and it is interesting to note how this fits in with the previouslypublished ... D kinase is controlled could provide newtherapeutic targets and strategies for the treatment ofcancer.The activity of the CDK4 ⁄ cyclin D holoenzyme is regulated by multiple mechanisms, the ... were performed using an AKTAFPLC (Amersham Biosciences, GE Healthcare, Amersham,UK). All chromatography columns and media were pur-chased from Amersham Biosciences unless otherwise stated.After...
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Tài liệu Báo cáo khoa học: Staphylococcal enterotoxin C1-induced pyrogenic cytokine production in human peripheral blood mononuclear cells is mediated by NADPH oxidase and nuclear factor-kappa B doc

Tài liệu Báo cáo khoa học: Staphylococcal enterotoxin C1-induced pyrogenic cytokine production in human peripheral blood mononuclear cells is mediated by NADPH oxidase and nuclear factor-kappa B doc

... Christian University, Tainan, Taiwan2 Department of Microbiology and Immunology, Medical College, National Cheng Kung University, Tainan, Taiwan3 Department of Medical Research, Chi-Mei Medical ... of staphylococcal enterotoxins causes emesisand diarrhea [4]. Staphylococcus aureus is also animportant microorganism of bovine, ovine and caprinemastitis [5]. Staphylococcal enterotoxins, especially ... cytosolic p47phox is phosphorylated andmoves to the membrane, where it binds to cyto-chrome b558and becomes an active oxidase [25–27].Another activation pathway for NF-jB is via 5-lip-oxygenase...
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Tài liệu Báo cáo khoa học: Aggregative organization enhances the DNA end-joining process that is mediated by DNA-dependent protein kinase pdf

Tài liệu Báo cáo khoa học: Aggregative organization enhances the DNA end-joining process that is mediated by DNA-dependent protein kinase pdf

... ligase 1 23456789101112131415MultimerTrimerDimerMonomer16 17 18 19 20 21 22 23 24 25 26 27 28 29 30DNA affinityfractionMultimerTrimerDimerMonomerMono QfractionATP+- ++++++++++++++++++++++++++Fig. ... nuclear matrix ⁄ scaffold attachment DNAelements. EMBO J 11, 3431–3440.18 Krasnow MA & Cozzarelli NR (1982) Catenation ofDNA rings by topoisomerases. Mechanism of controlby spermidine. ... including DNA-PKcs, SAF-A, Ku70 andhistone H1, following treatment with sequencing gradetrypsin (Promega, Madison, WI, USA), the fragmentalpolypeptides were isolated through reverse-phase columnchromatography...
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Báo cáo khoa học: Vanadium-induced apoptosis of HaCaT cells is mediated by c-fos and involves nuclear accumulation of clusterin pptx

Báo cáo khoa học: Vanadium-induced apoptosis of HaCaT cells is mediated by c-fos and involves nuclear accumulation of clusterin pptx

... Clusterin-mediated apoptosis is regulated by adenomatous polyposis coli and is p21dependent but p53 independent. Cancer Res 64,7412–7419.65 O’Sullivan J, Whyte L, Drake J & Tenniswood M(2003) ... apoptosis of HaCaT cells is mediated byc-fos and involves nuclear accumulation of clusterinSoultana Markopoulou1,*, Evangelos Kontargiris1,*, Christina Batsi1,*, Theodore Tzavaras2,Ioannis ... Japan) fitted with a camera(Nikon cool pix 990; Nikon Corp., Tokyo, Japan).DNA fragmentation assayDNA fragmentation analysis of VOSO4-treated anduntreated cells was performed as described previously[29,30]....
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Báo cáo khoa học: Transcriptional regulation of the desferrioxamine gene cluster of Streptomyces coelicolor is mediated by binding of DmdR1 to an iron box in the promoter of the desA gene doc

Báo cáo khoa học: Transcriptional regulation of the desferrioxamine gene cluster of Streptomyces coelicolor is mediated by binding of DmdR1 to an iron box in the promoter of the desA gene doc

... ‘com-mitting’ enzymes for secondary metabolites that haveevolved as variants of enzymes involved in primarymetabolism [23].All the evidence from this work indicates that thedesABCD cluster is expressed ... SpainIron is an essential element required for many keymetabolic processes (including cytochrome and Fe-Selectron transporters) in almost all micro-organisms.The bioavailability of iron is very ... in iron metabolism remains obscure. Thisputative siderophore-interacting protein is similar tothe Vibrio fischeri ViuB vibriobactin utilization protein[24].Primer extension analysis of the...
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Báo cáo khoa học: Deadenylation of interferon-b mRNA is mediated by both the AU-rich element in the 3¢-untranslated region and an instability sequence in the coding region pot

Báo cáo khoa học: Deadenylation of interferon-b mRNA is mediated by both the AU-rich element in the 3¢-untranslated region and an instability sequence in the coding region pot

... exosome [8]. This complexfirst discovered in yeast [9], is composed of proteins withribonuclease activity and is able to direct 3¢)5¢ mRNAdegradation. Therefore, the recruitment of the exosome ... with the Klenowpolymerase before oligomerization. The DNA fragmentswere cloned in the T4 DNA polymerase blunted PstIsiteofthe pSP65 vector. The length of the inserted fragments wasestimated ... respectively.Isolation of total RNA and RNase H treatmentTotal RNA was prepared by the Trizol method (Lifetechnologies, Inc.). RNase H treatment was performedaccording to the method described...
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Báo cáo Y học: Induction of chicken ovalbumin upstream promoter-transcription factor I (COUP-TFI ) gene expression is mediated by ETS factor binding sites doc

Báo cáo Y học: Induction of chicken ovalbumin upstream promoter-transcription factor I (COUP-TFI ) gene expression is mediated by ETS factor binding sites doc

... COUP-TFI andEts-1: the mesenchyme of t he bladder ( Fig. 4A±D), themesenchyme of the nasal septum (Fig. 4E±H), the cerebralcortex (Fig. 4I±L), the mesenchyme of vibrissae(Fig. 4M±P), spleen, ... 3D). Taken together, these experiments suggest thatEts-1 is able t o bind t he COUP-TFI promo ter preferentiallyat site C. This is not surprising because site C is the mostclosely related to ... containing this COUP-TFIpromoter fragment. Then we subdivided that segment intotwo s ubfragments ()397/)259 and )490/)408), eachcarrying three putative binding sites. The subfragment)397/)259 is not...
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Báo cáo khoa học: Proteolysis of the tumour suppressor hDlg in response to osmotic stress is mediated by caspases and independent of phosphorylation pot

Báo cáo khoa học: Proteolysis of the tumour suppressor hDlg in response to osmotic stress is mediated by caspases and independent of phosphorylation pot

... (%)GAPDHhDlg0361Time after sorbitol treatment (h)03 6 14Time after UV treatment (h)9DhDlg protein level (%)03691215020406080100CTime after sorbitol release (h) Time after UV treatment (h)Fig. ... early stage of apoptosis or cell attachment. Our findings showthat osmotic stress triggers hDlg degradation through a mechanism differ-ent from the one mediated by proteasomes, and we identify ... treatment is  40% higher in subconfluent cells than in confluentcells. This is probably because of the difference inhDlg localization observed. In confluent cells, hDlg is present mainly in the membrane,...
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