... that upon loss of the first metal ion, the three remaining metal ions in the a domain rear-range, either independently or in conjunction with the b domain, to position another metal ion into the ... reaction; in other words, coordination of the first metal ion facilitates the binding of the secondmetal ion, and so forth. Experimentally, this translatesinto the detection of only the initial ... investigated the metal-binding reactivity of the isolated a domain of human metallothionein isoform 1a, with specificemphasis on resolving the debate concerning the cooperative nature of the metal-binding...