... primer was 5´–TTAGAT CTC CAC CAT GCC CGG GCT GGG G–3´, and the downstream primer was 5´–AGA ATC ACA GCC CCT GCA A–3´. Single PCR products of the expected size (1.2 kb for BMP4 and 1.5 kb for ... blot detection of BMP4 (A), BMP6 (C and E), and β-actin (B and D). A solution of lyzed transduced Vero cells (A, B, C, and D) or conditioned medium (E) was loaded onto the reduced gel; A. BMP4 ... (Lanes 1, 2 and 3), BstXI (Lanes 4, 5, and 6), and BglII plus EcoRV (Lanes 7, 8, and 9). M, 1-kb DNA ladder. Lanes 1, 4, and 7, 293A cells; Lanes 2, 5, and 8, ADrBMP6 DNA; Lanes 3, 6, and 9, ADhBMP6...