Báo cáo Y học: Cloning and characterization of novel snake venom proteins that block smooth muscle contraction pptx

Báo cáo Y học: Cloning and characterization of novel snake venom proteins that block smooth muscle contraction pptx

Báo cáo Y học: Cloning and characterization of novel snake venom proteins that block smooth muscle contraction pptx

... Cloning and characterization of novel snake venom proteins that block smooth muscle contraction Yasuo Yamazaki 1 , Hisashi Koike 1 , Yusuke Sugiyama 1 , Kazuko Motoyoshi 1 , Taeko ... polylepis).Calciseptineisa known blocker of L-type Ca 2+ channels, a property that underlies its ability to block K + -induced contractions of aortic smooth muscle and spo...
Ngày tải lên : 18/03/2014, 01:20
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Báo cáo Y học: Cloning and characterization of the mammalian-specific nicolin 1 gene (NICN1) encoding a nuclear 24 kDa protein doc

Báo cáo Y học: Cloning and characterization of the mammalian-specific nicolin 1 gene (NICN1) encoding a nuclear 24 kDa protein doc

... Hassan Y. Naim 2 and Tosso Leeb 1 1 Institute of Animal Breeding and Genetics and 2 Institute of Physiological Chemistry, School of Veterinary Medicine Hannover; 3 Institute of Molecular Biology, ... RT-PCR analysis provided evidence that the murine Nicn1 gene is also expressed during development (Fig. 3). A more detailed analysis of the expression of the Nicn1 gene at da...
Ngày tải lên : 23/03/2014, 21:20
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Tài liệu Báo cáo khoa học: Cloning and characterization of CBL-CIPK signalling components from a legume (Pisum sativum) ppt

Tài liệu Báo cáo khoa học: Cloning and characterization of CBL-CIPK signalling components from a legume (Pisum sativum) ppt

... MgCl 2 without addition of any exogenous Ca 2+ . Phospho-amino acid analysis The phosphorylated 26-kDa band of PsCBL and the 58-kDa band of PsCIPK were separately eluted from the gel and hydrolysed in 6 n ... 7A, D, I and L are diamidino-2phenylindole hydrochloride (DAPI) stained cells showing the nuc- leus, and Fig. 7C, F, K and N are the merged images of A and B, D and...
Ngày tải lên : 19/02/2014, 07:20
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Tài liệu Báo cáo khóa học: Cloning and characterization of two distinct isoforms of rainbow trout heat shock factor 1 ppt

Tài liệu Báo cáo khóa học: Cloning and characterization of two distinct isoforms of rainbow trout heat shock factor 1 ppt

... formation Nobuhiko Ojima and Michiaki Yamashita Cell Biology Section, Physiology and Molecular Biology Division, National Research Institute of Fisheries Science, Fisheries Research Agency, Yokohama, Japan To ... cycle of 15 min at 95 °C, amplification cycles of 0.5 min at 94 °C, 0.5 min at 50 °C and 0.5 min at 72 °C, and one termination cycle of 1 min at 72 °C, with 35 cycles...
Ngày tải lên : 19/02/2014, 12:20
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Tài liệu Báo cáo Y học: Expression and characterization of active site mutants of hevamine, a chitinase from the rubber tree Hevea brasiliensis docx

Tài liệu Báo cáo Y học: Expression and characterization of active site mutants of hevamine, a chitinase from the rubber tree Hevea brasiliensis docx

... activity (Table 3). CONCLUSIONS In this study we investigated the catalytic role of Asp125, Glu127 and Tyr183 in hevamine by X-ray crystallographic and kinetic analysis of several mutants. We show that Glu127 ... (1995) Stereochemistry of chitin hydrolysis by a plant chitinase/lysozyme and X-ray structure of a complex with allosamidin: evidence for substrate assisted catalysis...
Ngày tải lên : 22/02/2014, 04:20
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Báo cáo khoa học: Cloning and characterization of the genes encoding toxic lectins in mistletoe (Viscum album L) pot

Báo cáo khoa học: Cloning and characterization of the genes encoding toxic lectins in mistletoe (Viscum album L) pot

... similar pattern of five bands, one of which was also more intensive than the others. Thus, the different intensity of the bands may be the result of a different copy number of the genes in the hybridizing ... sequences of the proteins encoded by the ml1p, ml2p, and ml3p genes are identical to MLI by 98, 88, and 77%, respectively. The similarity to MLI of the amino acid sequ...
Ngày tải lên : 07/03/2014, 15:20
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Báo cáo Y học: Cloning and expression of sterol D14-reductase from bovine liver potx

Báo cáo Y học: Cloning and expression of sterol D14-reductase from bovine liver potx

... Della Fazia 4 and Giuseppe Servillo 4 1 Department of Internal Medicine, University of Perugia, Italy; 2 Department of Pharmacological Sciences, University of Milan, Italy; 3 Department of Biochemical ... Universita Á ÔLa SapienzaÕ Roma, Italy; 4 Department of Biochemical Sciences and Molecular Biotechnology, University of Perugia, Italy Biosynthesis of cholesterol represe...
Ngày tải lên : 08/03/2014, 16:20
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Báo cáo Y học: Isolation and characterization of a thioredoxin-dependent peroxidase from Chlamydomonas reinhardtii doc

Báo cáo Y học: Isolation and characterization of a thioredoxin-dependent peroxidase from Chlamydomonas reinhardtii doc

... by HPLC and some of them were totally or partially analyzed by Edman sequencing and/ or by MALDI-TOF mass spectrometry ( Fig. 2). Computer database searches based on the amino-acid sequences of ... reductase system in yeast and animals, w hile 1Cys-Prx may be r educed by a small thiol molecule such as glutathione. R ecently, 1Cys-Prx has been identi®ed i n y east and Arabi dopsis...
Ngày tải lên : 08/03/2014, 16:20
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Báo cáo Y học: Expression and characterization of recombinant vitamin K-dependent c-glutamyl carboxylase from an invertebrate, Conus textile doc

Báo cáo Y học: Expression and characterization of recombinant vitamin K-dependent c-glutamyl carboxylase from an invertebrate, Conus textile doc

... carboxylase activity. The effect of proFIX18, proPT18 and pro-e-TxIX/12 on FLEEL carboxylation by the recombinant bovine carboxylase (closed symbols) and the recom- binant Conus carboxylase (open symbols) ... crude venom duct homogen- ate or bovine carboxylase (Table 3). Effect of the propeptides of vitamin K-dependent protein precursors on Conus carboxylase activity Our work [43]...
Ngày tải lên : 17/03/2014, 10:20
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Báo cáo Y học: Cloning and expression of two novel aldo-keto reductases from Digitalis purpurea leaves potx

Báo cáo Y học: Cloning and expression of two novel aldo-keto reductases from Digitalis purpurea leaves potx

... Biology (ZMBP), University of Tu ¨ bingen, Germany; 2 Department of Plant Biology, University of Valencia, Spain The aldo-keto reductase (AKR) superfamily comprises proteins that catalyse mainly ... superfamily [8]. The members of the AKR superfamily are cytosolic, monomeric proteins that catalyse mainly the NAD(P)H- dependent reduction of a wide variety of carbonyl com- p...
Ngày tải lên : 18/03/2014, 01:20
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