... Cloning and characterization of novel snake venom proteins
that block smooth muscle contraction
Yasuo Yamazaki
1
, Hisashi Koike
1
, Yusuke Sugiyama
1
, Kazuko Motoyoshi
1
, Taeko ... polylepis).Calciseptineisa
known blocker of L-type Ca
2+
channels, a property that
underlies its ability to block K
+
-induced contractions of
aortic smooth muscle and spo...
... Hassan Y. Naim
2
and Tosso Leeb
1
1
Institute of Animal Breeding and Genetics and
2
Institute of Physiological Chemistry, School of Veterinary Medicine Hannover;
3
Institute of Molecular Biology, ... RT-PCR
analysis provided evidence that the murine Nicn1 gene is also
expressed during development (Fig. 3). A more detailed
analysis of the expression of the Nicn1 gene at da...
... MgCl
2
without addition of
any exogenous Ca
2+
.
Phospho-amino acid analysis
The phosphorylated 26-kDa band of PsCBL and the
58-kDa band of PsCIPK were separately eluted from the
gel and hydrolysed in 6 n ... 7A, D, I and L are diamidino-2phenylindole
hydrochloride (DAPI) stained cells showing the nuc-
leus, and Fig. 7C, F, K and N are the merged images
of A and B, D and...
... formation
Nobuhiko Ojima and Michiaki Yamashita
Cell Biology Section, Physiology and Molecular Biology Division, National Research Institute of Fisheries Science,
Fisheries Research Agency, Yokohama, Japan
To ... cycle of 15 min at 95 °C,
amplification cycles of 0.5 min at 94 °C, 0.5 min at 50 °C
and 0.5 min at 72 °C, and one termination cycle of 1 min at
72 °C, with 35 cycles...
... activity (Table 3).
CONCLUSIONS
In this study we investigated the catalytic role of Asp125,
Glu127 and Tyr183 in hevamine by X-ray crystallographic
and kinetic analysis of several mutants. We show that
Glu127 ... (1995) Stereochemistry of chitin
hydrolysis by a plant chitinase/lysozyme and X-ray structure of a
complex with allosamidin: evidence for substrate assisted catalysis...
... similar
pattern of five bands, one of which was also more intensive
than the others. Thus, the different intensity of the bands
may be the result of a different copy number of the genes in
the hybridizing ... sequences of the proteins encoded by
the ml1p, ml2p, and ml3p genes are identical to MLI by 98,
88, and 77%, respectively. The similarity to MLI of the
amino acid sequ...
... Della Fazia
4
and Giuseppe Servillo
4
1
Department of Internal Medicine, University of Perugia, Italy;
2
Department of Pharmacological Sciences, University of Milan,
Italy;
3
Department of Biochemical ... Universita
Á
ÔLa SapienzaÕ Roma, Italy;
4
Department of Biochemical Sciences and Molecular Biotechnology, University of Perugia, Italy
Biosynthesis of cholesterol represe...
... by HPLC and some of them
were totally or partially analyzed by Edman sequencing
and/ or by MALDI-TOF mass spectrometry ( Fig. 2).
Computer database searches based on the amino-acid
sequences of ... reductase system in yeast
and animals, w hile 1Cys-Prx may be r educed by a small
thiol molecule such as glutathione. R ecently, 1Cys-Prx has
been identi®ed i n y east and Arabi dopsis...
... carboxylase activity. The effect of
proFIX18, proPT18 and pro-e-TxIX/12 on FLEEL carboxylation by
the recombinant bovine carboxylase (closed symbols) and the recom-
binant Conus carboxylase (open symbols) ... crude venom duct homogen-
ate or bovine carboxylase (Table 3).
Effect of the propeptides of vitamin K-dependent
protein precursors on
Conus
carboxylase activity
Our work [43]...
... Biology (ZMBP), University of Tu
¨
bingen, Germany;
2
Department of Plant Biology, University of
Valencia, Spain
The aldo-keto reductase (AKR) superfamily comprises
proteins that catalyse mainly ... superfamily [8].
The members of the AKR superfamily are cytosolic,
monomeric proteins that catalyse mainly the NAD(P)H-
dependent reduction of a wide variety of carbonyl com-
p...