... Calcium-binding by p26olf, an S100-like protein in the frog olfactoryepitheliumNaofumi Miwa, Yukiko Shinmyo and Satoru KawamuraFrom the Department of Biology, Graduate School of Science, Osaka University, ... in DEF-A and DEF-B, and small in DEF-C and DEF-D; this indicated that the affinities of EF-A and EF-B in the N-terminal half of wild-type p26olfare comparatively high, and those of EF-C and EF-D ... order to determine the affinity for Ca21 and thecooperativity of Ca21-binding to p26olf, we next analyzedthe stoichiometry of Ca21-binding to p26olf by flow dialysisin the Tris buffer. Ca21-binding...