... important active site residues(AsPPase numbering: E-125, K-133, E-135, R-155, Y-170,D-192, D-194, D-197, D-224, D-229, K-231, Y-269 and K-270) (Fig. 1), which have been reported previously ... staining), and that the inhibition of PPase-catalyzed PPihydrolysis by these inhibitors, is likely toprevent the synthesis of the new cuticle from the hypodermis and/ or ecdysis of the old cuticle. ... recombinantproteins was revealed by incubation of the filters withalkaline phosphate-conjugated goat anti-rabbit IgG (ICN) for 1 h and developed with 5-bromo-4-chloro-3-indolyl-phosphate/nitroblue tetrazolium...