... Statistical analy-sis was performed using instat software (GraphPad Soft-ware). The normality of the distribution of data wasevaluated using the Kolmogorov–Smirnov test. The equal-ity of the ... Niigata, Japan2 Kamiura Station, National Research Institute of Aquaculture, Fisheries Research Agency, Kamiura, Oita, Japan3 National Research Institute of Aquaculture, Fisheries Research Agency, ... invertebrates [25–27]. Mammalian transferrin canalso bind various trace metals, including manganese,copper, and zinc, although the affinity of transferrin for these trace metals is lower than that for...