... mL of the abovementioned culture medium: a total of 400 dishes wereprepared and used in the further steps of the experiments.Fungal materialFod pathotype 2 was used in the experiments, as the ... from the tissues of carnation (Dianthuscaryophyllus L). The enzyme, with a molecular mass of 43–45 kDa and a pI of 4.15, specifically methylates the hydroxy substituent in 4¢-position of the flavones, ... water and represented the control. After, respectively, 24, 48 and 72 h of culture under the growth conditions already specified for the in vitromaterial, the calluses were collected and used in the following...