Báo cáo khoa học: Substrate recognition and ®delity of strand joining by an archaeal DNA ligase docx

... Substrate recognition and ®delity of strand joining by an archaeal DNA ligase Masaru Nakatani1,2, Satoshi Ezaki1,2, Haruyuki Atomi1,2 and Tadayuki Imanaka1,21Department of Synthetic ... release of free AMP from the DNA adenylate[2,5,6,13].Catalytic activity of DNA ligase is dependent onappropriate divalent cations and DNA substrates. Ingeneral, DNA ligases can utilize M g2+ and ... of oligonucleotides recognized by LigTkas DNA substrates. At 80 °C, LigTkcould ligateoligonucleotides of nine nucleotides or more as L -DNA with an R -DNA of 30 nucleotides, and an R -DNA of...

Báo cáo khoa học: Substrate recognition by three family 13 yeast a-glucosidases Evaluation of deoxygenated and conformationally biased isomaltosides pptx

... after (Fig. 2B,C) addition of the enzyme.Table 2. Kinetic parameters and DDGà for hydrolysis of isomaltose and p-nitrophenyl-a-D-glucopyranoside, and mono-deoxy analogs of methyla-isomaltoside ... was found in isomaltosehydrolysis by glucoamylase, which depended on enzyme± substrate transition-state interactions with OH-4 and -3 of an energy of 16.5 and 8.6 kJámol)1, respectively (Table ... Substrate recognition by three family 13 yeast a-glucosidasesEvaluation of deoxygenated and conformationally biased isomaltosidesTorben P. Frandsen1,*, Monica M. Palcic2 and Birte...

Báo cáo khoa học: Substrate preference and phosphatidylinositol monophosphate inhibition of the catalytic domain of the Per-Arnt-Sim domain kinase PASKIN ppt

... Eckhardtà, Emanuela Borter§, Patrick Spielmann and Roland H. WengerInstitute of Physiology and Zu¨rich Center for Integrative Human Physiology, University of Zu¨rich, SwitzerlandKeywordsmetabolism; ... against S6 and the GST-tag. (C) Immunoblot analysis of thephosphorylation status of p70S6K and S6 inPaskin+ ⁄ + and Paskin) ⁄ )MEFs. (D) Immu-noblot analysis of the phosphorylation status of p70S6K ... G. Thomas and S. C. Kozma for the generous gifts of plasmids and cell lines, as well as Gieri Camenisch and DanielP. Stiehl for helpful discussions. This work wassupported by by grants from...

Báo cáo khoa học: Substrate recognition by glycoside hydrolase family 74 xyloglucanase from the basidiomycete Phanerochaete chrysosporium pptx

... (5¢-TTTGCGGCCGCTCATAGACACAAATTGCCGGFig. 7. MALDI-TOF MS analysis of peaks A (A) and B (B) in Fig. 6.The peaks were fractionated and analyzed by MALDI-TOF MS.Peak A included oligosaccharides of DP 16–18, and peak B includedoligosaccharides ... Reading, PA, USA).HPLC and MALDI-TOF MS analysisThe reaction products of TXG and XXXGXXXG gener-ated by Xgh74B treatment were analyzed by normal-phaseHPLC and MALDI-TOF MS. The oligosaccharide,XXXGXXXG, ... Xylp and Galp indicateD-glucopyranose, D-xylopyranose and D-galactopyranose, respectively.Fig. 2. Effect of deglycosylation on puriﬁed Xgh74B. Lane 1: mole-cular weight standards (kDa). Lane...

Tài liệu Báo cáo khoa học: Afﬁnity and kinetics of proprotein convertase subtilisin ⁄ kexin type 9 binding to low-density lipoprotein receptors on HepG2 cells docx

... unlabeledwild-type as well as by two mutant variants of PCSK9 and LDL, but not by an unrelated ligand, suggestingthat they all compete with [125I]TC-PCSK9 for thesame receptor.Linear [27–29] and curvilinear ... or withunlabeled ligand or medium (pH 6.2), and dissociation of speciﬁcallybound ligands was followed as a function of time. Data are pre-sented as percentage of total ligand bound at zero time ... of lipo-proteins, and there was a corresponding increase in thebinding of [125I]TC-PCSK9 and [125I]TC-PCSK9-D374Y. Second, the extent of speciﬁc binding of both[125I]TC-labeled ligands...

Tài liệu Báo cáo khoa học: Identiﬁcation and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebraﬁsh, Danio rerio docx

... expression of T-bet has beenseen within a wide variety of tissues and cells in theGinbuna crucian carp [27] and, in mammals, in thelung tissue of mouse and spleen and thymus of human and mouse ... [44].The gene organizations of zebrafish t-bet, stat6 and foxp3 were also determined and found to be very simi-lar to their human homologues. Both the human and zebrafish T-bet and Foxp3 genes ... 2009 FEBS 131t-bet, stat6 and foxp3 gene organization and chromosome syntenyUsing the zebrafish t-bet, stat6 and foxp3 cDNAsequences elucidated by PCR and the regions of thezebrafish genome that...

Tài liệu Báo cáo khoa học: Pronounced adipogenesis and increased insulin sensitivity caused by overproduction of prostaglandin D2 in vivo pptx

... musclewith organ, but without fat, of WT and H-PGDS TG mice (n = 6). Continuousdissections of mouse fat and bone in thewhole body were quantiﬁed by use of amicro-CT scanner and LATHETA software(Aloka). ... the32P-labeledfull-length cDNA for human H-PGDS. (C)Immunohistochemical analysis of transgeneexpression in the liver and WAT. Parafﬁnsections of liver and WAT from WT and TGmice (S55) were stained with antibodyagainst ... epididymal and perirenal fat, BAT, and liver.Values are expressed as means ± SEMs.*P < 0.05, **P < 0.01 as compared withWT mice. (D) Changes in the weights of visceral and subcutaneous fat and...

Tài liệu Báo cáo khoa học: Molecular cloning and characterization of soybean protein disulﬁde isomerase family proteins with nonclassic active center motifs pdf

... Expression of GmPDIL-3a and GmPDIL-3b in soybeantissues. (A) Puriﬁed recombinant GmPDIL-3a (lanes 1 and 4),GmPDIL-3b (lanes 2 and 5) and proteins extracted from the cotyle-don (lane 3) were analyzed ... (80 mg bean) (lane 1), root (lane 2), stem (lane 3), leaf(lane 4) and ﬂower (lane 5) were analyzed by western blot usinganti-GmPDIL-3a serum.ABFig. 4. Localization of GmPDIL-3a and GmPDIL-3b ... that the 53 and 57 kDa spots areGmPDIL-3a and GmPDIL-3b, respectively. Samplesfrom different parts of the soybean plant werethen prepared and analyzed by western immunoblot.GmPDIL-3a and GmPDIL-3b...

Tài liệu Báo cáo khoa học: SREBPs: physiology and pathophysiology of the SREBP family ppt

... addition to antiplatelet and coagu-lant actions, PUFAs also inhibit lipogenesis and lowertissue and plasma triglyceride levels through inhibition of SREBP-1c. The molecular mechanisms by whichPUFAs ... activate, and PUFAs suppress, SREBP-1c, leading to and protecting from hepatic insulin resistance and pancreatic b-cell insulin secretion, respectively [39]. FA, fatty acid.H. Shimano Physiology and ... monitor and controller of cell membrane composition.Pathophysiological aspects of SREBPsin various organsAccumulation of lipids has been linked to functionaldisturbances in various tissues and...

Tài liệu Báo cáo khoa học: Identiﬁcation and characterization of an R-Smad ortholog (SmSmad1B) from Schistosoma mansoni pdf

... 40G14,9E14, 51H3, and 6H19. The BAC DNA was isolated, and the presence of the SmSmad1B sequence was conﬁrmed by PCR and by sequencing the BAC DNA. Exon–intron siteswere located by aligning the cDNA sequence ... Drosophila MAD and the vertebrate Smad1,Fig. 1. Structure of the SmSmad1B gene, cDNA and protein. (A) Schematic representations of the SmSmad1B gene, cDNA and protein and the amino acid sequence of SmSmad1B ... arthropods, and ver-tebrates. The same results were obtained by maximumlikelihood and neighbor -joining distance analyses (sup-plementary Figs S1 and S2).SmSmad1B gene structure and 5¢ upstreamanalysisThe...

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