Báo cáo khoa học: Expression of the Pycnoporus cinnabarinus laccase gene in Aspergillus niger and characterization of the recombinant enzyme pdf

... Expression of the Pycnoporus cinnabarinus laccase gene in Aspergillus niger and characterization of the recombinant enzyme Eric Record1, Peter J. Punt2, ... pLac1-A and pLac1-B contained the laccase cDNA corresponding to the laccase gene, lac1 from P. cinnabarinus (GenBankaccessionnoAF170093) (Fig. 1). In pLac1- B, the 21 amino acids of the laccase ... construction in o rder to study the timecourse of the laccase activity.Study of the recombinant laccase production in A. niger For both expression v ectors, the laccase activity was found in the culture...

Báo cáo khoa học: "A Stacked Sub-Word Model for Joint Chinese Word Segmentation and Part-of-Speech Tagging" potx

... furtherused in POS tagging in (Ng and Low, 2004). Onemain disadvantage of this model is the difﬁculty in incorporating the whole word information. The second kind of solution is the “word-based”method, ... SegC and SegTagLare the predictions of the three coarse-grained solvers. For the three words at the begin-ning and the two words at the end, the three predic-tors agree with each other. And these ﬁve ... an f-score of 94.02 for the whole task, resulting in relative error reductions of 14.1% and 5.5% respectively over the best systemreported in the literature. The remaining part of the paper is...

Tài liệu Báo cáo khoa học: Various secretory phospholipase A2 enzymes are expressed in rheumatoid arthritis and augment prostaglandin production in cultured synovial cells docx

... moderate staining in the interstitium (purple arrows). (C–E) Staining of sPLA2-V in three active RA tissues. In all cases, intense staining of the granulation tissue in the sublining interstitium ... staining of sPLA2-IIE was observed in two inactive RA sections (Fig. 4A), it was intense in VSMC in three distinct active RA sections (Fig. 4Ba–c). In contrast, staining of synovial lining ... present in the joints of patients with rheumatoid arthritis(RA), expression of other sPLA2s in this disease has remained unknown. In this study, we examined the expression and localization of six...

Báo cáo khoa học: G protein-coupled receptor-induced Akt activity in cellular proliferation and apoptosis pptx

... activation of protein kinase C and the mobilization of intracellular Ca2+, leading to the formation of a complex containing Src-family kinases, the focal adhesion kinase, Pyk2, and PI3K [33].Similar ... promote the expression of the tumour necrosis factor (TNF) receptor-associated death domain and of the TNF-relatedapoptosis-inducing ligand, leading to the activation of death-receptor signaling and ... factor and the directphosphorylation and activation of the cAMP-responseelement binding protein. These two transcription fac-tors have been implicated in the promotion of the expression of genes...

Tài liệu Báo cáo khoa học: Expression and function of Noxo1c, an alternative splicing form of the NADPH oxidase organizer 1 doc

... phosphoinositide binding[31]. The five-amino-acid insertion in the loop of Noxo1 might alter the configuration of the phospho-inositide-binding region, affecting the affinity for Expression and function ... in the testis and fetal brain. The Noxo1c protein contains anadditional five amino acids in the N-terminal PX domain, a phosphoinosi-tide-binding module; the domain plays an essential role in ... directly involved in the interaction with phospho-inositides [30,31]. The loop region in the PX domain of p40phoxfaces the phosphoinositide-binding pocketas shown by the crystal structure of the...

Báo cáo khoa học: Expression and characterization of the protein Rv1399c from Mycobacterium tuberculosis A novel carboxyl esterase structurally related to the HSL family docx

... into t he expression vectorfollowing the manufacturer’s instruction (Gateway, Invi-trogen). The correct insertion of the Rv1399c ORF wasconﬁrmed by DNA sequencing. In our hands, protein expression ... [23].Results and DiscussionCloning and expression of Rv1399c The Rv1399c gene product encodes a 318 amino acidprotein with a molecular mass of  31.7 kDa and acalculated pI of 4.53 and belongs to the ... that of acetylcholinesterase (30 mM). The kinetic behavior of Rv1399c using short-chain soluble vinylesters and triacylglycerols along with the lack of d etectableactivity using insoluble vinyl...

Báo cáo khoa học: Expression of the Drosophila melanogaster ATP synthase a subunit gene is regulated by a transcriptional element containing GAF and Adf-1 binding sites pptx

... included the mitochondrial single-stranded binding protein (mtSSB), and the catalytic (a )and accessory (b) subunits of the DNA polymerase c (P ol c)[24,25]. Interestingly, the expression of the genes ... identiﬁcation of severaltranscription factors and coactivators that regulate the expression of genes playing key r oles in the biogenesis of the OXPHOS system. In general, the transcription of nucleargenes ... processing of the D. melanogastera-F1-ATPasetranscript The expression of the a-F1-ATPase gene during develop-ment is coordinated with the expression of the nuclear-encoded b-F1-ATPase gene and...

Báo cáo khoa học: Expression analysis of the nucleocytoplasmic lectin ‘Orysata’ from rice in Pichia pastoris ppt

... calculated using the forceﬁeld of discover3 and used to anchor the pyranose ring of the sugars into the binding sites of the lectin. The position of mannoseobserved in the Man–banana lectin complex ... the cytoplasm and the nucleoplasm, including the nucleo-lus (Fig. 1).Puriﬁcation and characterization of recombinant Orysata expressed in Pichia pastorisCloning of the coding sequence of Orysata into the Escherichia ... the N-ter-minus as well as the histidine tag at the C-terminus of the recombinant lectin apparently do not in uence the biological activity of Orysata, since the recombinant lectin reacted with...

Báo cáo khoa học: Expression of the pyrG gene determines the pool sizes of CTP and dCTP in Lactococcus lactis doc

... regulation of gene expression and modulation of kinetic properties of enzymes emphasizes the need for tight regulation of the level of nucleotides in the cell. Indeed, expression of genes responsible ... the only reaction resulting in the amination of the pyrimidine ring into a cytosine derivative (Fig. 1). It istherefore of interest to examine to what extent this enzyme controls the ﬂuxes and ... increase in the CTP concentration in strains withincreased pyrG expression of up to 250% of the wild-typelevel (Fig. 4A). The results show that the feedbackinhibition of the CTP synthase enzyme...

Tài liệu Báo cáo khoa học: Expression and secretion of interleukin-1b, tumour necrosis factor-a and interleukin-10 by hypoxia- and serum-deprivation-stimulated mesenchymal stem cells Implications for their paracrine roles ppt

... 2010)doi:10.1111/j.1742-4658.2010.07770.xTo understand the potential paracrine roles of interleukin-1b (IL-1b),tumour necrosis factor-a (TNF-a) and interleukin-10 (IL-10), the expres-sion and secretion of these factors by rat ... transplantation in rat models of myocardial infarction has anti -in ammatory effects,decreasing protein production and gene expression forIL-1b and TNF-a [11]. To address these paradoxes of both pro- and ... IL-10Because of the lack of secretion of the in ammatorycytokines IL-1b and TNF-a from hypoxia ⁄ SD-stimu-lated MSCs, as well as the signiﬁcant anti -in amma-tory effects of MSCs, expression and secretion...

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