Báo cáo Y học: Oxidation of propionate to pyruvate in Escherichia coli Involvement of methylcitrate dehydratase and aconitase pot
Báo cáo Y học: Oxidation of propionate to pyruvate in Escherichia coli Involvement of methylcitrate dehydratase and aconitase pot
... (2001) In vitro con-version of propionate to pyruvate by Salmonella enterica enzymes:2 -methylcitrate dehydratase (PrpD) and aconitase enzymes cata-lyze the conversion of 2 -methylcitrate to 2-methylisocitrate.Biochemistry ... reconstitution of the oxidation of propionyl-CoA to pyruvate by the use of purified PrpC, PrpD, AcnB and PrpB from E. coli. PrpD and AcnB involved in the conversion of methylcitrate into2-methylisocitrate ... purification of one of the key enzymes of the methylcitrate cycle, methylcitrate synthase, led to theidentification of an operon necessary for propionate degra-dation. In E. coli and S. enterica...
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Tài liệu Báo cáo Y học: Soluble silk-like organic matrix in the nacreous layer of the bivalve Pinctada maxima A new insight in the biomineralization field pptx
... Proline, hydroxyproline and hydroxylysine were examined at 254 nm by reverse-phaseHPLC of their phenylisothiocarbamate derivatives [26], asreported previously [20]. The serine, threonine and tyrosinecontents ... fromanion-exchange (AE) HPLC of water-soluble matrix (WSM) and EDTA-soluble matrix (EDTA-SM). Cysteine, hydroxylysine, hydroxy-proline, phosphoserine, proline and tryptophan were not determined.Amino acid AE-WSM ... Hydroxyproline, hydroxy-lysine and phosphoserine were not detected in WSM, WIM,EDTA-SM and EDTA-IM.The comparison of the amino acid composition of themain peak obtained from AE-HPLC of WSM and...
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Báo cáo khoa học: Succinate dehydrogenase flavoprotein subunit expression in Saccharomyces cerevisiae – involvement of the mitochondrial FAD transporter, Flx1p ppt
... confirmed byprobing cellular extracts with an antibody against theflavin moiety of covalently flavinylated proteins(a-FAD). Following western blotting analysis, a bandFig. 2. (A) Succinate dehydrogenase ... activity of mitochondrial riboflavin trans-port system(s) and two enzymes, riboflavin kinase(EC 2.7.1.26) and FAD synthetase (EC 2.7.7.2), whichcatalyze the synthesis of FMN and FAD respectively[10–13]. ... transport in Saccharomyces cerevi-siae. It has also been suggested that it has a role in maintaining the normalactivity of mitochondrial FAD-binding enzymes, including lipoamidedehydrogenase and...
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Báo cáo khoa học: Enhancing the protein production levels in Escherichia coli with a strong promoter potx
... solubility of a protein, such asfolding velocity and hydrophobicity. When proteinsare produced, the synthesis rate of the protein mayaffect the proportion of soluble protein. Previously, itwas ... Jenny Ottosson and Sophia HoberSchool of Biotechnology, Department of Proteomics, Royal Institute of Technology, AlbaNova University Center, Stockholm, SwedenIntroductionRecombinant protein ... class in vitro. The proteins withhigher protein solubility class are more likely to yield ahigher amount of soluble protein. Interestingly, thiscorrelation is independent of the choice of promoter.Comparison...
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Báo cáo y học: "A Practical Approach to Management of Chronic Hepatitis B"
... bbiiooggrraapphh y y Ke-Qin Hu, MD, is the Director of Hepatology Services and Associate Professor of Clinical Medicine, Divisions of Gastroenterology and Transplantation, University of California, Irvine, ... treatment [16]. 4. Indications of HBV Treatment Historically, levels of HBV DNA and ALT, and histological activity of liver biopsy have been used as the three main factors to determine if a patient ... infection and active hepatic inflammation carry a significantly higher risk of disease progression. Histological staging of HBV disease is clinically very valuable in assessing degree of fibrosis and...
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Tài liệu Báo cáo Y học: Oxidation of phenols by laccase and laccase-mediator systems doc
... N-hydroxyphtalimide (HPI) and 2,2,6,6-tetramethylpiperidin-1-yloxy free radical (TEMPO) asmediators, since they greatly differ both in their mechan-ism of action and in their specificity. In ... Roma, Italy To investigate how solubility and steric issues affect thelaccase-catalysed oxidation of phenols, a series of oligomericpolyphenol compounds, having increasing size and decreasing solubility ... and 4-tert-butylphenol being bis-hydroxymethylated. The purity of these compounds (> 96%) was checked by1HNMR and HPLC.Spectrophotometric determination of laccase activityThe activity of laccase...
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Báo cáo Y học: Human bile salt-stimulated lipase has a high frequency of size variation due to a hypervariable region in exon 11 pot
... fat-soluble vitamin esters and cholesterolesters in the small intestine. In some species, includinghumans, the gene is also expressed in the lactatingmammary gland and the resulting protein is a constituent of ... exocrine pancreas in all species examined. BSSL has a broad substratespeci®city a nd contributes to the hydrolysis of dietarymono-, di-, a nd tri-acylglycerols and is responsible fordigestion of ... activation, heparin binding,heat stability, stability at low pH and resistance to proteolytic inactivation [16±18].The BSSL protein i s well c onserved between species, butthe number of proline-rich...
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Báo cáo y học: "Pre-hospital intubation by anaesthesiologists in patients with severe trauma: an audit of a Norwegian helicopter emergency medical service"
... included the type of airway device and drugs used to facilitate ETI, complications and the use of monitoring,including capnography. We anonymised the involvedHEMS physicians and recorded them ... conse-quences of delaying ETI until arrival in the ED. A recentstudy from the Netherlands [20] also showed a failure to adhere to guidelines for pre-hospital ETI in traumaticbraininjuryinalmosthalfofthestudiedpopulation.Furthermore, ... more likely to survive thanthose intubated pre-hospital. Although this difference in injury severity may explain our findings, we believe thatfurther studies are needed to clarify the clinical...
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Báo cáo y học: "Maternal Outcomes According to Placental Position in Placental Previa"
... According to Placental Position in Placental Previa Dong Gyu Jang, Ji Sun We, Jae Un Shin, Yun Jin Choi, Hyun Sun Ko, In Yang Park, Jong Chul Shin Department of Obstetrics and Gynecology, ... hemoglobin level of prior to surgery, 1 day after surgery, and 3 days after surgery, the amount of transfusion during surgery, estimated blood loss during operation, pla-cental accreta, hysterectomy, ... uterine myoma, increased BMI, increased neonatal weight, and complete previa [3-5]. Also, risk factors for peripartum hysterectomy are previous cesarean section, history of abortion, and complete...
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Báo cáo y học: "MALDI-TOF MS Combined With Magnetic Beads for Detecting Serum Protein Biomarkers and Establishment of Boosting Decision Tree Model for Diagnosis of Colorectal Cancer"
... useful tool for integrating separation and analysis of complex mixtures of proteins. Captured proteins are then analyzed by TOF-MS, generating a spectral map depicting approximations of the ... may also be involved. In conclusion, MALDI-TOF MS combined with magnetic beads is one useful tool for integrating se-paration and analysis of complex mixtures of pro-teins. With the panel of ... expression of protein mass peaks (P < 0.01) was listed by the software. Subsequently, the differen-tiated expressions of protein mass peak were ana-lyzed by discriminatory analysis. Briefly, the...
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Từ khóa: báo cáo y họcbáo cáo y học cổ truyềnbáo cáo khoa học y họcbáo cáo y tế học đườngmẫu báo cáo y tế học đườngbáo cáo y tế học đường cuối nămBáo cáo quy trình mua hàng CT CP Công Nghệ NPVchuyên đề điện xoay chiều theo dạngNghiên cứu tổ chức pha chế, đánh giá chất lượng thuốc tiêm truyền trong điều kiện dã ngoạiBiện pháp quản lý hoạt động dạy hát xoan trong trường trung học cơ sở huyện lâm thao, phú thọGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANQuản lý hoạt động học tập của học sinh theo hướng phát triển kỹ năng học tập hợp tác tại các trường phổ thông dân tộc bán trú huyện ba chẽ, tỉnh quảng ninhPhát triển du lịch bền vững trên cơ sở bảo vệ môi trường tự nhiên vịnh hạ longPhát hiện xâm nhập dựa trên thuật toán k meansNghiên cứu, xây dựng phần mềm smartscan và ứng dụng trong bảo vệ mạng máy tính chuyên dùngNghiên cứu về mô hình thống kê học sâu và ứng dụng trong nhận dạng chữ viết tay hạn chếNghiên cứu tổng hợp các oxit hỗn hợp kích thƣớc nanomet ce 0 75 zr0 25o2 , ce 0 5 zr0 5o2 và khảo sát hoạt tính quang xúc tác của chúngChuong 2 nhận dạng rui roQuản lý nợ xấu tại Agribank chi nhánh huyện Phù Yên, tỉnh Sơn La (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtNguyên tắc phân hóa trách nhiệm hình sự đối với người dưới 18 tuổi phạm tội trong pháp luật hình sự Việt Nam (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtĐổi mới quản lý tài chính trong hoạt động khoa học xã hội trường hợp viện hàn lâm khoa học xã hội việt namTÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲ