Báo cáo khoa học: Relationships between the ethanol utilization (alc ) pathway and unrelated catabolic pathways in Aspergillus nidulans pptx

... ifL-isoleucine and L-valine are degraded in A. nidulans as theyare in yeast, then the formation and conversion of the Fig. 2.D-Galacturonic acid provokes induction of the alc genes.(A) Northern ... of the alcA and alcR genes (NI *) [13]. (B) Putrescine-induced transcription of the alcA and gabA genes in the leaky putrescine utilization mutant punA11compared to that in a wild type strain. ... the acetyl-CoA synthetasegene (facA) showed that inducing amounts of acetate hadbeen formed from the ethanol applied to lactose-grownbiomass during the induction period as well as in the ethanol- grown...

Tài liệu Báo cáo khoa học: Relationships between structure, function and stability for pyridoxal 5¢-phosphate-dependent starch phosphorylase from Corynebacterium callunaeas revealed by reversible cofactor dissociation studies doc

... mM ) was present from the b eginning of the r econstitution or was added at the e ndof the burst phase (5 min). The yield was the same in bothexperiments within t he experimental error. The ... incubated in the resolution buffer at 30 °Cusing protein concentrations in the range 0.5–2.0 m gÆmL )1 until the residual activity w as between 1.5 and 2 .5% o f the original leve l. The r esolution ... phos-phorolysis and s ynthesis, and assays for P L-phosphory-lase in the synthesis direction contained a saturating levelof activating phosphate (2.5 mM ). Results are shown in Fig. 6. In either direction...

Báo cáo khoa học: Relationship between the structure of guanidines and N-hydroxyguanidines, their binding to inducible nitric oxide synthase (iNOS) and their iNOS-catalysed oxidation to NO pptx

... iNOSFEBS Journal 272 (200 5) 3172–3183 ª 2005 FEBS 3177binding of an N-hydroxyguanidine moiety in the iNOSheme pocket thus roughly involves the same bindingenergy as binding of the guanidine ... pharmacological interest[8,9].Apart from some equilibrium and kinetic constantsrelated to the binding of l-Arg [10–14] and NOHA[10], nothing is known about the thermodynamics and kinetics of the binding ... Substrate speciﬁcity of iNOSRelationship between the structure of guanidines and N-hydroxyguanidines, their binding to inducible nitricoxide synthase (iNOS) and their iNOS-catalysed oxidationto...

Báo cáo khoa học: Discriminating between the activities of human cathepsin G and chymase using ﬂuorogenic substrates potx

... (199 7) Speciﬁcinhibition of thrombin-induced cell activation by the neutrophil proteinases elastase, cathepsin G, and pro-teinase 3: evidence for distinct cleavage sites within the aminoterminal ... 2) and ABZ-GIATFRMLMPEQ-EDDnp (substrate 3) were similar in the 2 · 102mm )1 Æs )1 range (Table 1), indicating that the Lys40 in chymase does not act as a discriminatingstructural determinant ... yellow. (B) Ribbon plot of CG and chymase in irreversible complexes with syn-thetic inhibitors showing ball -and- stick mod-els for the seven residues located in the vicinity of the active site. The...

Báo cáo khóa học: Interaction between the 2¢)5¢ oligoadenylate synthetase-like protein p59 OASL and the transcriptional repressor methyl CpG-binding protein 1 potx

... of the MBD1 gene was induced by interferon,indicating that these genes are co-ordinately regulated. The interaction was confirmed in vitro and in vivo and wasmapped to the ubiquitin-like domain ... Translation of the methyl CpG-bindingprotein 1 splice variant 6 (MBD1v 6). The methylCpG-binding domain (MBD or TAM),Zn-finger domains (CxxC1- 3), nuclear local-ization signal (NLS) and the transcriptionalrepression ... of the bacterial LexAgene and the human p59 OASL. To identify proteinsthat interact with specific domains of p59 OASL, deletionmutants containing the P-loop, ATP-binding, and UbLdomains, individually...

Báo cáo khoa học: "Reading Between the Lines: Learning to Map High-level Instructions to Commands" ppt

... interacting with the en-vironment and observing the resulting feedback,our method automatically learns both the mapping between the text and the commands, and the un-derlying model of the environment. ... task.Instead, we use the training documents to guide the sampling process. During training, we execute the command sequences predicted by the policyfunction in the environment, caching the resultingstate ... example mapping of a document containing high-level instructions into a candidate se-quence of ﬁve commands. The mapping process involves segmenting the document into individual in- struction...

Tài liệu Báo cáo khoa học: Role of the cag-pathogenicity island encoded type IV secretion system in Helicobacter pylori pathogenesis pptx

... (1 1). In this manner, the bacteria could access integrins and inject CagA (1 2). Injected CagA can then induce the massive disruption of cell junctions(1 3) and a loss of cell polarity (1 4). The ... in future stud-ies. Taken together, CagAPYinteracts with a surpris-ingly high number of host proteins to induce signalling pathways involved in cell scattering, elongation and probably other ... hybridscreens and other in vitro binding studies indicated that the very carboxy-terminus interacted with integrin b1[13], which has been proposed to be cytoplasmic [10](Fig. 2C). Thus, the role of the...

Tài liệu Báo cáo khoa học: Bioimaging of the unbalanced expression of microRNA9 and microRNA9* during the neuronal differentiation of P19 cells doc

... hairpin are involved in formation of the piR-NA-interacting complex (piRC) and function in the transcriptional gene silencing of retrotransposons and genetic elements in germline cells [18].Investigations ... gener-ation, and to be related to the expression ofgranuphilin ⁄ slp4 in insulin-producing cells [28]. More-over, miR9 and other neuronal miRs includingmiR125b and miR128, are involved in the Alzheimer’disease ... region of the pri-miR9-1was randomly split into ﬁve different segments by PCR, )1 387 to )4 4 bp (miR9-1PN1_Fluc), )8 46 to )4 4 bp(miR9-1PN2_Fluc), )5 30 to )4 4 bp (miR9-1PN3_Fluc), )2 36 to )4 4 bp...

Tài liệu Báo cáo khoa học: Characterization of chitinase-like proteins (Cg-Clp1 and Cg-Clp2) involved in immune defence of the mollusc Crassostrea gigas docx

... IUÆmL )1 ) and penicillin G(100 IUÆmL )1 ), and were incubated (CO2-free) at 15 °C.Haemocyte monolayers were then treated for 30 min withculture medium containing bacterial LPS (1 lgÆlL )1 in NaCl ... by incubating sections in 0.3% hydrogen peroxide in methanol for 30 min at room temperature. Slides werethen washed and incubated in a blocking solution accord-ing to the manufacturer’s instructions. ... the oyster Crassostrea gigas [5]. Interestingly, thisprotein, named C. gigas chitinase-like protein 1 (Cg-Clp 1) was found to be involved in the control ofgrowth and remodelling processes in...

Tài liệu Báo cáo khoa học: Oligomerization of the Mg2+-transport proteins Alr1p and Alr2p in yeast plasma membrane pptx

... protein–proteininteractionUsing the split-ubiquitin system, we investigated the interaction of the C-terminally truncated Alr1 isomersAlr-c36, Alr-c63 and Alr-c96. The protein lacking the very C-terminus of Alr1p ... hetero-oligomeric interactions.Accordingly, we conclude that both the N-terminus and C-terminus of Alr1 and Alr2 are in the same com-partment, i.e. in the cytoplasm. A N -in, C -in orienta-tion ... JS74-B (alr1D) cells expressing ALR1 , ALR2 and ALR2R768E from a CEN (YCp) plasmid. The cells were incubated in medium containing Mg2+(mM) as indicated for 12 h before deter-mination of the Mg2+concentration....

Xem thêm