Báo cáo khoa học: Localization of ﬂuorescence-labeled poly(malic acid) to the nuclei of the plasmodium of Physarum polycephalum pot

Báo cáo khoa học: A steady-state modeling approach to validate an in vivo mechanism of the GAL regulatory network in Saccharomyces cerevisiae ppt

... expression. The status of the switch is determined by the state of the upstream regulatory element of the GALgenes in the nucleus. The regulatory protein may bind to the upstream regulatory element constituting ... couldinﬂuence the binding of the regulatory protein to the other,representing cooperativity [5,6]. A regulatory protein maybe the product of the same genetic switch that it regulatesand i s termed a utoregulation ... withdimer G al80p in the cytoplasm alone without translocating to the nucleus. The consequent nucleocytoplasmic shuttling of the Gal80p monomer from the nucleus to the cytoplasmrelieves repression...

Tài liệu Báo cáo khoa học: Localization of N-linked carbohydrate chains in glycoprotein ZPA of the bovine egg zona pellucida pptx

... SDS/PAGE. To detect the shift of the 21 kDaband, 2.5 mU of N-glycanase was added to 4 lg (56 pmol) of ZPA. To detect the shift of the 63 kDa band, 0.25 mU of N-glycanase was added to 1 lg (14 pmol) of ... glycosylated.Determination of the number of N-glycosylation sitesWe then investigated the number of N-glycosylation sitesand the localization of N-linked chains in ZPA using the puriﬁed ZPA. In the 15% gel, the 21 ... activity[10] and these active chains are mainly localized in the N-terminal region of the ZP domain of ZPB and the C-terminal region of the ZP domain of ZPC (Fig. 5). In the cow, ZPB exhibits the strongest...

Báo cáo khóa học: Localization, puriﬁcation and properties of a tetrathionate hydrolase from Acidithiobacillus caldus pot

... hydrolase of A. caldus (Eur. J. Biochem. 271) 275Chemical stability of potassium tetrathionate the substrate of tetrathionate hydrolase To quantitate the substrate using the HPLC method, the effect of ... stableunder these conditions at temperatures of up to 80 °C(datanot shown). The interference of ammonium sulfate with the HPLC-assay of tetrathionate was also tested. The height of the tetrathionate ... Dialysis of the crude enzyme extract precipitated about 20% of the totalprotein, probably because hydrophobic proteins aggregatedin the presence of the high NaCl concentration (1M). Most of the...

Báo cáo khoa học: Localization of ﬂuorescence-labeled poly(malic acid) to the nuclei of the plasmodium of Physarum polycephalum pot

... on the amount of the probes injected, the phase of the cell cycle, and the position of the covalent linkage of the dye to the polymer. Resultswere indistinguishable for the low (white) and the ... Localization of fluorescence-labeled poly(malic acid) to the nuclei of the plasmodium of Physarum polycephalum Miachael Karl1, Bernd Gasselmaier1, ... is thought to belong to pieces of halos not focussed to the plane. The occurrencetogether of the green halo and the red fluorescence wasverified in more than 90% of the fluorescent nuclei. Thus,...

Tài liệu Báo cáo khoa học: NirF is a periplasmic protein that binds d1 heme as part of its essential role in d1 heme biogenesis pdf

... d1heme-bindingdomain of cytochrome cd1. Therefore, we tested for the binding of d1heme to puriﬁed NirF. The addition of d1heme to the NirF resulted in the appearance of adistinctive visible ... NirF and a range of nucleotide-containing cofactors by using a variety of biophysical methods (data not shown). Nonetheless,we still decided to test the effect of the deletion of the entire GXGX2GX7G ... NirF can be realized in the cytoplasm of Ps. aeruginosa, with the resulting d1heme then being translocated to the periplasm. In the case of P. pantotrophus it would be the substrate forNirF...

Tài liệu Báo cáo khoa học: High-afﬁnity ligand binding by wild-type/mutant heteromeric complexes of the mannose pptx

... mini-receptors was measured to assess whether the presence of the mutant mini-recep-tors affected PMP-BSA binding to the wild-type mini-receptors (Fig. 5C). In these experiments, the nullhypothesis ... domain but,rather, there are interactions that exist between dimerpartners all along the ectodomain of the receptor.Collectively, these studies led to the hypothesis thatproduction of high-afﬁnity ... solubleforms of the receptor [25,26,34], indicates that the ectodomain of the receptor is capable of dimer forma-tion in the absence of ligand.Afﬁnity cross-linking of [125I]IGF-II to a mutantrepeat...

Tài liệu Báo cáo khoa học: Collagen I regulates matrix metalloproteinase-2 activation in osteosarcoma cells independent of S100A4 pdf

... wereattached on top of or inside the ﬁbrillar 3D matrix(data not shown). Some of the synthesized MMP-2was adsorbed to the ﬁbrillar collagen, and the ratio of active to total MMP-2 was the same as ... surface of the cell that pro-duced them. The ECM can be found in differentforms, from the hard compositions of bone to the softstructures of connective tissue. Collagens are the maincomponents of ... TIMP-1 inhibited the second step that is anautoactivation of the intermediate 64 kDa form to the fully active 62 kDa enzyme. Further, autoactivation of the active 62 kDa form to a C-terminally...

Tài liệu Báo cáo khoa học: 2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5¢-phosphate synthases of fungi and archaea docx

... reduction of the ribosyl side chain of compound 3 in the ascomy-cete Ashbya gossypii is conducive to introduction of ahydrogen atom into the 1¢-proS position of the ribitylside chain of riboﬂavin ... carbon atoms of the ribosylside chain [12]. Moreover, the signals of the side-chain carbon atoms of 13C-labelled compound 3appear as multiplets due to 13C13C coupling. Treat-ment of the 13C-labelled ... wereshown to be A-type reductases catalysing the transfer of deuterium from the 4(R) position of NADPH to the 1¢ (S) position of the substrate. Theseresults are in agreement with the known three-dimensional...

Tài liệu Báo cáo khoa học: Evidence for noncooperative metal binding to the a domain of human metallothionein ppt

... initially to the a domain, thereby lead-ing to formation of the M4a cluster prior to formation of the M3b cluster, demonstrating that the individualbinding constants of divalent metal ions for the a ... in efforts to elucidate the potentially cooper-ative nature of the metal-binding reaction within each of the domains. Thus, the goal is to elucidate the meta-llation mechanisms of the individual ... of the a domain of rhMT-1a, showing the connectivities of the four divalent metal cations to the 11 cystei-nyl sulfurs. The numbering of the cysteines(1–11 starting from the N-terminus) and the four...

Tài liệu Báo cáo khoa học: DNA modiﬁcation with cisplatin affects sequence-speciﬁc DNA binding of p53 and p73 proteins in a target site-dependent manner pptx

... and the ability of the particular p53target site to accommodate the cisplatin IACs. The higher the probability of formation of the cisplatinIACs within the p53DBSs due to the occurrence of the ... within p53DBS of the competitorbut not within the p21b target; see (ii)]. The graphs show the relative binding of p53 to the radiolabeled targets as a function of rb; the intensities of the R53bands ... mixture of the p21b target with the pPGM1 competitor fragmentwas treated with cisplatin in the same way, the inten-sity of the R53band on the autoradiogram increasedwith the degree of DNA...

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