... they arehydrophobic and negatively charged. A comparison of rhodamine uptake, in the presence and the absence of uncouplers, allows the estimation of the effect of intracellular binding on the ... altered by NBD-Cl. On the other hand,NBD-Cl enhanced the fluorescence of TMRM takenup into nonrespiring cells, but had little effect on the distribution of the dye within the cells.NBD-Cl is ... whose mitochondria had beenpoisoned to rhodamines, quasi-equilibrium is achieved within 1 h. Contrary to our expectations, under theseconditions, the free concentration of the dyes in the cytoplasm...