Báo cáo khoa học: "Should we Translate the Documents or the Queries in Cross-language Information Retrieval?" ppt

Tài liệu Báo cáo khoa học: Inhibitory properties of cystatin F and its localization in U937 promonocyte cells ppt

... more weakly, with Ki¼ 33 nm,comparable to the inhibition of the aminopeptidasecathepsin H (Ki¼ 30 nm ). In comparison with othercystatins, cystatin F is a rather slow binding inhibitorof ... cystatin F we have studied the in vitro kinetics of the interactionbetween cystatin F and several cathepsins, as wellas their intracellular localization in promonocyteU937 cells, using speciﬁc ... occupies the S3pocket in cystatin C is the mostdiscriminating residue for binding to cathepsins B, H,L, S [24]. No L9K mutants of cystatin C have beenprepared yet to study the effect of incorporating...

Tài liệu Báo cáo khoa học: How to remain nonfolded and pliable: the linkers in modular a-amylases as a case study ppt

... avoided (Table 1).There is therefore a much weaker driving force forfolding the connecting linkers when compared with aglobular protein. In addition, the main polaruncharged side chains (Asn, Gln, ... near the N- and C-termini of the linkers: this suggests thata mobile connection with both the catalytic domainand the binding module is required for the function of the nanomachine. Furthermore, ... bondhydrolyzed has the theoretical capacity to disrupt ahydrophobic interaction in the linker, inducing or favoring its extension. As the catalytic constant kcatofanimal-type a-amylases is in the range...

Tài liệu Báo cáo khoa học: Membrane targeting and pore formation by the type III secretion system translocon pdf

... assembly. In the ﬁrst model, both hydrophobic translocators exist in oligomeric form, with the major partner insertedstably into the membrane, whereas the minor protein is the link with the V antigen. ... PopB in P. aeruginosa, IpaB in Shigella spp. andEspD in pathogenic E. coli spp.), whereas the smallestprotein (i.e. the minor translocator; YopD, PopD,IpaC and EspB in the aforementioned organisms) ... strain remains unable to translocate toxins [19],suggesting that the major hydrophobic translocatorrequires a minor translocator for functional transloconformation. In some cases, major translocator...

Tài liệu Báo cáo khoa học: Accessibility changes within diphtheria toxin T domain when in the functional molten globule state, as determined using hydrogen⁄deuterium exchange measurements pdf

... chaincorresponding to the catalytic domain (C domain);and the B chain corresponding to the translocationdomain (T domain) and the receptor-binding domain. The C and T domains remain covalently linked ... toinsertion of the toxin in the membrane. The C domainis then translocated across the endosomal membraneinto the cytosol. The C domain ADP-ribosylates the elongation factor 2, blocking protein translation ... TH8 is in the corebecause it is buried in the structure. Within the wholetoxin, TH5 is partly covered by the C-terminal part of the receptor (R) domain [2,3]. Within the isolated Tdomain, this...

Tài liệu Báo cáo khoa học: Multifunctional host defense peptides: Antimicrobial peptides, the small yet big players in innate and adaptive immunity docx

... attacking the microbe membrane, and in the intracellular com-partment, they contribute to the oxygen-independentkilling of phagocytosed microorganisms. Furthermore,defensins are mediators in the ... a chemokine involved in homeo-static lymphocyte homing as well as in epithelial cellmigration, further suggesting a function for hBD2 in healing and protection of the intestinal epithelialbarrier ... different cohorts of cells to the site of injury, antimicrobial peptides indirectlyfavor chemotaxis by inducing or increasing the secre-tion of chemokines. For example, LL-37 has beenshown to induce...

Tài liệu Báo cáo khoa học: Reversible tetramerization of human TK1 to the high catalytic efﬁcient form is induced by pyrophosphate, in addition to tripolyphosphates, or high enzyme concentration ppt

... [27–29]. In the light of these effects, the complex regulatoryand structural properties of hTK1 may be importantfor maintaining a balanced supply of the DNA precur-sor. This underlines the importance ... in the following G1phase impliespredominance of the low-activity dimer form. As the hTK1 concentration increases during S phase, moreand more enzyme will be in the high-active tetramerform. ... dimericforms in Figs 1 and 2 exhibited low or high catalyticefﬁciency. Therefore, the various incubated hTK1forms from Figs 1 and 2 were analyzed for theirkinetic behavior with dThd, and the results...

Tài liệu Báo cáo khoa học: Olfactory receptor signaling is regulated by the post-synaptic density 95, Drosophila discs large, zona-occludens 1 (PDZ) scaffold multi-PDZ domain protein 1 pptx

... anyinteraction with the PDZ domains investigated. We furthermore investigated the binding determi-nants in the C-terminus of hOR2AG1 by spotting pep-tides that correspond to mutated or shortened ... 5C). In summary, when the interaction betweenMUPP1 and OR2 AG1 is inhibited by over-expressing the interacting PDZ domains, the resulting response of OR2 AG1 to odorant is modiﬁed in that the rapiddecay ... receptors contain putative PDZbinding motifs in their C-termini, following the previ-ously outlined rules of binding [16]. PDZ domains1 + 2 of MUPP1 indeed showed direct interactionwith OR C-terminal...

Tài liệu Báo cáo khoa học: A knowledge-based potential function predicts the speciﬁcity and relative binding energy of RNA-binding proteins ppt

... Starting with eachcomplex in the training set containing one or more RRM or KH domains, we extracted the protein coordinates and the four nucleotides bound at the center of the domain (forstructures ... contact-counting score; the blue line represents the rank of thesesequences using the distance-dependent potential. The points in each colored line are sorted independently by rank; the x-axis is the sort ... combined training ⁄ test set.Thus, to assess the performance of the potential withoutbiasing the result, the native structure of scored complexeswas excluded from the training set for the score...

Tài liệu Báo cáo khoa học: Helix mobility and recognition function of the rat thyroid transcription factor 1 homeodomain – hints from 15N-NMR relaxation studies pdf

... and Gln59 in the C-terminal end ofhelix III, are located apart from the major cluster,towards the lower intercept of the theoretical curveand ﬁtting line (Fig. 4, left inset), where the motion ... only within helix III,but also over a large portion of the protein, from Phe8to Met56, including the loop and the tight turn. At the same time, in the vicinity of the Arg53 hinge point,precisely ... dis-tribution of the energy between the overall tumblingand the internal mobility, and is indicative of the degree of internal restraint of each15N–1H vectormotion. In Fig. 4, most of the points cluster...

Tài liệu Báo cáo khoa học: Pyrimidine-speciﬁc ribonucleoside hydrolase from the archaeon Sulfolobus solfataricus – biochemical characterization and homology modeling doc

... triﬂuoroacetic acid in H2O. The retention times of adenosine and adenine, inosine and hyp-oxantine, guanosine and guanine, uridine and uracil, cyti-dine and cytosine were 12.2 and 6.2 min, ... carried out by incu-bating recombinant SsCU-NH with proteinase K, subtili-sin, trypsin or thermolysin. Enzymatic digestion withproteinase K, subtilisin or trypsin was performed in 10 mm Tris ... accordingto Facchiano et al. [48].Docking of ribose and Ca2+into the binding site ofSsCU-NH was performed using, as a starting reference, the position of the sugar and of the metal in the...

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