... -CCATGTTTCTAGAGGCTCTGCAGCGTGCTATCCCGCCTGAGGTCAAGTT-3¢; rAATG9,5¢-CCATGTTTCTAGAGACCGTTGACTACGCTATCCCGCCTGAGGTCAAGTT-3¢, rACTE8,5¢-TACCGCGGTCAAAATCCTGCAGCGTGCTATCCTGGT GGAGACGCGTGA-3¢ and rACTG9,5¢-TACCGCGGTCAAAACCGTTGACTACGCTGCTCTGGTGGAGACGCGTGA-3¢. ... (Roche, Mannheim, Germany; Amer-sham Pharmacia, Piscataway, USA; Promega, Buchs,Switzerland), anti-His antibody and alkaline phosphatase-conjugated goat antimouse secondary antibody (Sigma,Buchs, ... 5¢-TTGTTGAACTTGACCTCAGG-3¢ forAAT variants and 5¢-GTACCGCGGTCAAA-3¢ and 5¢-TCACGCGTGTCCAC-3¢ for ACT variants. Resulting PCRfragments were cloned as KasI ⁄ Bsu36I fragments into pAATand as MluI ⁄ SacII...