Báo cáo khoa học: Isolation, characterization and expression analysis of a hypoxia-responsive glucose transporter gene from the grass carp, Ctenopharyngodon idellus potx

Tài liệu Báo cáo khoa học: Cloning, characterization and expression analysis of interleukin-10 from the common carp, Cyprinus carpio L. docx

... Cloning, characterization and expression analysis of interleukin-10 from the common carp, Cyprinus carpioL.Ram Savan1, Daisuke Igawa2 and Masahiro Sakai21United Graduate School of Agricultural ... GAGGCTAGATACTGCTCGATGTIL-10 forward3 TGATGATTTGGAACCATTATTGAAIL-10 reverse3 CACCTTTTTCCTTCATCTTTTCATb-Actin forward1 ACTACCTCATGAAGATCCTGb-Actin reverse1 TTGCTGATCCACATCTGCTGT7- forward TAATACGACTCACTATAGGGSP6-reverse ... wereacclimatized in an aerated fresh water tank at 20 °C, under a natural photoperiod, and fed for 2 weeks, prior to use in the study.Cloning and characterization of the carp IL-10 gene A carp...

Tài liệu Báo cáo khoa học: Physicochemical characterization and biological activity of a glycoglycerolipid from Mycoplasma fermentans ppt

... iscleaved enzymatically, and the product can be measuredphotometrically. This was carried out on an ELISAreader (Rainbow, Tecan, Crailsham, Germany) at a wavelength of 450 nm, and the values ... temperature until all free waterwas evaporated. After this, IR spectra were recorded atroom temperature and at 37 °C. Usually, the originalspectra were evaluated directly and a spectral analysis ... oxide and prostaglandin E2 as well asaugmented glucose utilization and lactate formation [11].These data were supported by others [13,14]. From these ﬁndings, the elucidation of molecularmechanisms...

Báo cáo khoa học: Identiﬁcation and expression analysis of an IL-18 homologue and its alternatively spliced form in rainbow trout (Oncorhynchus mykiss) doc

... CGACATTTCCGAGTGACGTTC 3¢-RACER2 CCTTCAACACCCTGACTTCAC 5¢-RACER3 ATGTCCTCCTTGTCTACTACC 5¢-RACEEF1 AGCAGCTCCGAATGTAAGGTG IL-1 8A expression EF2 GCTCCGAATTCGAACATGAC IL-18B expression ER1 AGGCAAAGGTTGCTCCAGTG ... AGGCAAAGGTTGCTCCAGTG IL-18 expression Actin-F ATGGAAGATGAAATCGCC Gene expression Actin-R TGCCAGATCTTCTCCATG Gene expression RR1 TGGTACCACTCAACATGTCAGTAAGCG Gene organization1914 J. Zou et al.(Eur. ... Sugawara, S., Uehara, A. , Nochi, T., Yamaguchi, T., Ueda, H.,Sugiyama, A. , Hanzawa, K., Kumagai, K., Okamura, H. &Takada, H. (2001) Neutrophil proteinase 3-mediated induction of bioactive...

Tài liệu Báo cáo khoa học: Biochemical characterization and inhibitor discovery of shikimate dehydrogenase from Helicobacter pylori docx

... enzymatic characterization of HpSDH demonstrates its activity with kcat of 7.7 s)1 and Km of 0.148 mmtoward shikimate, kcat of 7.1 s)1 and Km of 0.182 mm toward NADP, kcat of 5.2 s)1 and ... the shikimate path-way, which is essential for the synthesis of importantmetabolites, such as aromatic amino acids, folic acid, and ubiquinone [10]. The shikimate pathway is crucialto algae, ... (sense:5¢-GCGCATCCATATGAAATTAAAATCGTTTGG-3¢ and antisense: 5¢-CCGCTCGAGAAAAACGCTTCGCATGAC-3¢) were synthesized to clone aroE gene from H. pylori strainSS1. The following protocol was conducted for ampliﬁca-tion:...

Tài liệu Báo cáo khoa học: Molecular characterization and allergenic activity of Lyc e 2 (b-fructofuranosidase), a glycosylated allergen of tomato pdf

... some serato a 9- and a 15-kDa band. We could show that the 9-kDa band in the tomato extracts reacts with a specificantibody against the LTP from cherry, Pru av 3 and the 15-kDa band shows reactivity ... 5¢TATGCGTGGTCCAATGCTATGC, and FF 3A, matchingwith the C-terminal sequence of the coding region: 5¢TTACAAGGACAAATTAATTGTGCCAG. For amplification of the long isoform the same 5¢ primers were used, the 3¢specific ... 2003glycan analysis of the natural protein from tomatorevealed that MMXF is the dominating glycan withabout 84% of all sugar structures. The structures of the N-glycans of nLyc e 2 and their...

Báo cáo khoa học: Enzymatic characterization and molecular modeling of an evolutionarily interesting fungal b-N-acetylhexosaminidase pot

... morethan 0.3 nm.Most (83.5%) of the amino acid residues are plottedin the favourable regions of the Ramachandran plot. The deviation of geometrical parameters from idealvalues (G-factors) ... hydrolysis of N-formyl, N-glycolyl and N-propionyl derivatives of the standard N-acetylsubstrate) than A. oryzae (2–5% hydrolysis). The cloning and sequencing of PoHex and analysis of the corresponding ... between the sequence of the signal pep-tide of the PoHex and other hexosaminidases. Largepropeptides appear to be unique for the subfamily of fungal Hex and also appear to diverge relatively rap-idly...

Tài liệu Báo cáo khoa học: Complex transcriptional and translational regulation of iPLA2c resulting in multiple gene products containing dual competing sites for mitochondrial or peroxisomal localization docx

... TGGAAAGCTTGCCACATCAGTCTACAAAG85R TGCTCCATGGTGGCATCCCAATATGTAAACCA83 83F GAACCAAGCTTGAAGCACATTCTTGCAGTAAGCA83R CAAAACATGTTGGCTACGGGACATACAAATGTTCA80 80F GTTGAAGCTTTTTGAAACTTAGCACTTCTGC80R ATTCCATGGTGGCTGAAATCATTTCATTTTGATTGCC74 ... synthesized and annealed: g50 (5¢-TATTAATCTGACTGTAGATATATATATATTACCTCCTTAGTAATGC-3¢) and random-ized control g50c (5¢-TTGATAGTTATCTATTACAGTCTTCTTAGATTGAAACAA-3¢), g177 (5¢-CATACAAACATAATAAGATGTAAATGG-3¢) ... iPLA2c sequence:P1, 5¢-TCAAGGTACCATGATTTCCTGAAGG-3¢;P2,5¢-CTGAAGATCTAGCCTTTACTTTCA-3¢;P3,5¢-GCTAGGTACCAATACAGTAATATATG-3¢;P4,5¢-TGCTAGATCTCCACCCACTCA-3¢;P5,5¢-TTATGGTACCTGAAAGGGAATAGCGGC-3¢;P6,5¢-GGCTGGTACCCTTGCGCTCCGTC-3¢;P7,5¢-GGAGAGATCTGCGGGAAGCCGCGACAGA-3¢;p8,5¢-TTCCAGAT...

Tài liệu Báo cáo khoa học: Puriﬁcation and cDNA cloning of a cellulase from abalone Haliotis discus hannai ppt

... CTGATCTAGAGGTACCGGATCC5RACF ATCCTCACGAACAAGCAG5RACR GATCGCGATGCAGGCCTTFLF1 GGACGACTACAGCGTCTTCAGTAGAFLR1 TCCAAACAGTCAGTTTCTTAACCGTTable 1. Puriﬁcation of cellulase from abalone Haliotis discus hannai. ... of the cDNA library and cloning of cellulasecDNA was achieved as follows: Total RNA was extracted from 1 g of abalone hepatopancreas by the ganidiniumthiocyanate-phenol method [30] and mRNA ... Tokyo, Japan) and used as an abalone cDNAlibrary. cDNAs encoding abalone cellulase were ampliﬁedby PCR from the cDNA library with degenerated primerssynthesized on the basis of partial amino-acid...

Báo cáo khoa học: Crystal structure and enzymatic properties of a bacterial family 19 chitinase reveal differences from plant enzymes pdf

... chitinaseshave catalytic domains that are at least as large asthose of the plant enzymes and that may contain atleast six subsites [26,30]. However, the catalyticdomains of ChiG, ChiC and most other ... 1084–1092.35 Kawase T, Yokokawa S, Saito A, Fujii T, Nikaidou N,Miyashita K & Watanabe T (2006) Comparison of enzymatic and antifungal properties between family 18 and 19 chitinases from S. coelicolor ... the a- anomer and the b-anomer at equilibrium. The otherpanels show the results of partial hydrolysis of (GlcNAc)6 and (Glc-NAc)5by chitinase G (ChiG). In these panels, substrates display the 60...

Báo cáo khoa học: Puriﬁcation and structural analysis of the novel glycoprotein allergen Cyn d 24, a pathogenesis-related protein PR-1, from Bermuda grass pollen pot

... 27693QEGYGSTQLPSDEPLNGLNDCAA GAA GGA TAT GGC AGC ACC CAA CTT CCA TCG GAC GAG CCA TTG AAC GGG CTC AAC GAC 336113KAIQDILNEHNMFRAKERVPAAG GCC ATA CAG GAC ATC CTC AAC GAG CAC AAC ATG TTC CGC GCC AAG GAG CGC GTC CCG ... GAG CAG GTA GCG TCC GAG TCA GCC TCC CTC ACA ACA CCA 21673TTREEQPAAEAAASTAGGSQACC ACC AGG GAA GAA CAA CCG GCG GCA GAG GCC GCG GCG TCC ACC GCT GGC GGT AGC CAA 27693QEGYGSTQLPSDEPLNGLNDCAA GAA ... 516173SGAISWKTTVDTWSDEKKSYTCC GGC GCC ATC TCC TGG AAG ACG ACG GTG GAC ACG TGG AGC GAC GAG AAG AAG AGC TAC 576193HYGSNTCDQGKMCGHYTAVVCAC TAC GGC TCC AAC ACC TGC GAC CAA GGC AAG ATG TGC GGC CAC TAC...

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