Báo cáo khoa học: Amino acid residues on the surface of soybean 4-kDa peptide involved in the interaction with its binding protein potx
Báo cáo khoa học: Amino acid residues on the surface of soybean 4-kDa peptide involved in the interaction with its binding protein potx
... identify the residues of the 4-kDa peptide involved in the interaction with the 43-kDa protein, alanine-scanning mutagenesis of the 4-kDa peptide was performed. The 4-kDa peptide- expressionsystem in ... obtained of aromatic andhydrophobic residues emphasized the importance of these amino acids in the interaction between the 4-kDa peptide and the 43-kDa protein. The secondary structures of thesetwo ... the 43-kDa protein and the 4-kDa peptide/ 43-kDa protein complex. (A) Chromatogram of the 43-kDa protein. (B) Chromatogram of the 4-kDa peptide and the 43-kDa protein com-plex. The elution points of the...
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Tài liệu Báo cáo khoa học: Amino acid discrimination by arginyl-tRNA synthetases as revealed by an examination of natural specificity variants doc
... December2008)doi:10.1111/j.1742-4658.2009.06866.xl-Canavanine occurs as a toxic non -protein amino acid in more than 1500leguminous plants. One mechanism of its toxicity is its incorporation intoproteins, replacing l-arginine and giving rise ... the guanidinium g-N but alsocomes into contact with the adenosine ring of Ade76.There is a general consensus that tRNA binding is notrequired for arginine binding [33], although arginine binding ... not align with anycharged residues in the jack bean or soybean, leavingone to speculate on the source of the interaction with the sugar–phosphate backbone. Correct positioning of the essential...
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Báo cáo khoa học: Amino acid limitation regulates the expression of genes involved in several specific biological processes through GCN2-dependent and GCN2-independent pathways ppt
... digestion of proteins [4,5].Another example of the detection of the lack of an amino acid is metabolic adaptation to cope with epi-sodes of protein malnutrition. In these circumstances, amino acid ... involved in the up- anddown-regulation of gene expression in response to amino acid starvation.Results Amino acid starvation triggers changes in geneexpression In order to identify amino acid- regulated ... distribution of the amino acid- regulated genes* versus the distribution of the genes spotted onto the microarray**. *Percentage of the representation of one GO term among all the amino acid- regulated...
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Báo cáo khoa học: Glutamic acid residues in the C-terminal extension of small heat shock protein 25 are critical for structural and functional integrity pptx
... Frequency of amino acid residues in the flexible region of the C-terminal extensions of human and murine sHsps. Residues present in the flexible region of the C-terminal extension of each of the eight ... domains are known to be involved in the organization of the quaternary structure of sHsps and interaction with their target proteins, the role of the C-terminal extension is enigmatic.Despite the ... interactions [13]. Target proteins are held in a folding-competent conformation until conditionsare permissive for their refolding or degradation, with the former requiring the input of another...
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Báo cáo khoa học: Amino acid biosynthesis and metabolic flux profiling of Pichia pastoris ppt
... text), its reactions are depicted in grey. The amino acids and the carbon fragments originatingfrom a single intermediate of the central carbon metabolism are represented in rectangular boxes. Thin ... fed to the culture in a fed-batch mode. During this secondgrowth phase, the production of the recombinant protein isinduced by methanol, which activates the AOX1 promotercontrolling the heterologous ... lines between the amino acid carbon atomsdenote carbon bonds that are formed between fragments originating from different precursor molecules, while thick lines indicate the intact carbonconnectivities...
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Báo cáo khoa học: "Word classification based on combined measures of distributional and semantic similarity" docx
... Performance of the 3 versions of KNN in terms of precision: (1) without weighting of neighbors ; (2) with weighting by their distributional similarity to the test word and (3) with weighting by their ... describes the learning accuracy of these three versions of KINN (fi was set to 1).Table 1 compares them on the data of the twocorpora (the number in parentheses specifies the k for which the evaluation ... semantic information: the classification decisionis a function of both (1) the distributional similarity of the new word to the target class and (2) the strength of the semantic relatedness of the...
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Báo cáo khoa học: "Structural Disambiguation Based on Reliable Estimation of Strength of Association" potx
... Experiment Disambiguation of dependency re- lations was done using 75 anlbiguous con- structions from Fukumoto (1992). Solving the ambiguity in the constructions involves choosing among two or more ... relations Closest attachment (1) has a low perfor- mance since it fails to take into consideration the identity of the words involved in the deci- sion. Selecting the lowest classes (2) often ... pairs of classes corre- sponding to the head of a prepositional phrase and weighted them to bias the computation of the association in favor of higher-frequency co-occurrences which he considered...
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Báo cáo khoa học: "Using Corpus Statistics on Entities to Improve Semi-supervised Relation Extraction from the Web" pot
... relations definitions. 3 Description of SRES The goal of SRES is extracting instances of speci-fied relations from the Web without human super-vision. Accordingly, the supervised input to the ... to the specifications of the target relations. A specification for a given relation con-sists of the relation schema and a small set of seeds – known true instances of the relation. In the ... mentions of all instances of the given relation type inside a document or inside a collection of documents. Whereas, in the Web Extraction (WE) tasks we are only interested in extracting relation...
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Tài liệu Báo cáo khoa học: X-ray crystallographic and NMR studies of pantothenate synthetase provide insights into the mechanism of homotropic inhibition by pantoate docx
... of pantoate bound in the ATP -binding pocket. Pantoate binding to the ATP -binding site induced large changes in structure, mainlyfor backbone and side chain atoms of residues in the ATP binding HXGH(34–37) ... pan-toate protein interactions in the canonical pantoate- binding site (site I).Fig. S4. Two-dimensional projection showing the pan-toate protein interactions in the ATP -binding site(site ... substrates of a reaction has also been observed in the case of ade-nylosuccinate synthetase, one of whose substrates,IMP, binds to the GTP -binding site and inhibits the reaction [37–39]. The binding of...
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Tài liệu Báo cáo khoa học: A second novel dye-linked L-proline dehydrogenase complex is present in the hyperthermophilic archaeon Pyrococcus horikoshii OT-3 pptx
... respectively. The N-terminal amino- acid sequence of the a2 subunit corresponded to the underlined amino- acid sequence within MEIVRINEH-PILD from the putative protein encoded by the predic-ted ... contrast, contained only oneADP binding motif spanning residues 108–136 (Fig. 3),which suggests the additional ADP -binding motif in the a subunit might be essential for the dye-NADHDHactivity of the ... the ADP -binding motif in the a1 subunit mediates the observed ATP binding, and FMN is likely situated at the interface between the a1 and b1 subunits within the PDH1 complex. The dependence of...
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