... performed using a BigDyeÒ Terminator v3.1 Cycle Sequencing Kit (Perkin-Elmer ⁄ Applied Biosystems, Foster City, CA, USA) and anABI PRISMÒ 3100 Genetic Analyser (Perkin-Elmer ⁄Applied Biosystems).Protein ... was cloned using thezero blunt TOPO-cloning kit (Invitrogen, Carlsbad, CA,USA). The resulting plasmid was transformed into E. coliTOP10 competent cells and the insert was sequenced using a series ... mmTris ⁄ HCl, concentrated using Centricon P-20 units(Millipore) and stored at 4 °C.Protein purity was analysed by SDS-PAGE. Protein con-centrations were determined using the Bradford micro-assay(Bio-Rad,...