Báo cáo khoa học: The chloroplast ClpP complex in Chlamydomonas reinhardtii contains an unusual high molecular mass subunit with a large apical domain doc

... chloroplast ClpP complex in Chlamydomonas reinhardtii contains an unusual high molecular mass subunit with a large apical domain Wojciech Majeran1, Giulia Friso2, Klaas Jan van Wijk2and Olivier Vallon11 ... Cyanobacteria[22] and in the genome of the Cyanophora cyanelle, an ancestral chloroplast. In the green alga Chlamydomonas reinhardtii and in vascular plants, the plastid clpP1 gene is essential and cannot ... genes, as well between the algal CLPR6 and the CLPP6 of higher plants, indicates that the hetero-oligomeric organization of the ClpP complex wasestablished early in the green lineage, and maintainedby...

Tài liệu Báo cáo khoa học: The calcium-induced switch in the troponin complex probed by ﬂuorescent mutants of troponin I doc

... isolated TnC arewell known. Sites III and IV in the C -domain (carboxyterminal) bind Ca2+ with higher affinity, while sites I and II in the N -domain (amino terminal) bind Ca2+ with loweraffinity ... are sensitive to Ca2+binding to the two classes of sites, and the authors interpreted the high affinity sites being in the C -domain and the low affinity in the N -domain of TnC. Nevertheless an ... large size and potential forforming or disrupting interactions. The incorporation of5HW and other non-naturally occurring amino acid analogsinto a protein seems to be a good alternative. They...

Tài liệu Báo cáo khoa học: The occurrence of hemocyanin in Hexapoda docx

... 23crustacean hemocyanins, 4 crustacean pseudohemocyanins,20 chelicerate hemocyanins, 5 myriapod hemocyanins and1 onychophoran hemocyanin in the ﬁnal alignment, whichwas manually adjusted with the aid ... BduHc2Periplaneta americana Blattaria Blattidae Juvenile, adult PamHc1 PamHc2Shelfordella lateralis Blattaria Blattidae Juvenile, adult SlaHc1 SlaHc2Graphosoma lineatum Hemiptera Pentatomoidae Adult ... includedselected insect hexamerins, crustacean hemocyanins,crustacean pseudohemocyanins, chelicerate hemocya-nins, myriapod hemocyanins and one onychophoranhemocyanin in the ﬁnal alignment (Fig. S18). The phylogenetic...

Tài liệu Báo cáo khoa học: The sequentiallity of nucleosomes in the 30 nm chromatin ﬁbre pptx

... similar abundances andcan be assessed quantitatively) in the dinucleosomesample is indistinguishable from that of high- molecular weight chromatin. Furthermore, in the DNase I foot-print of the ... that approximately 90% of the DNA in the main peak as well as the mononucleo-somes and band S are all in the 140–160 bp sizeinterval (not shown). The high percentage of mono-nucleosomes obtained ... of allsizes with a single maximum. In their gels, there aretwo maxima. As shown in Fig. 3A, B of Dorigo et al.[14], both the even multiples (tetra- and hexamers) aremore abundant than the...

Tài liệu Báo cáo khoa học: The bacterium, nontypeable Haemophilus inﬂuenzae, enhances host antiviral response by inducing Toll-like receptor 7 expression ppt

... CYLD in air-way epithelial A5 49 and NHBE cells in vitro and in vivo.As shown in Fig. 5A, B, NTHi up-regulated CYLD in A5 49 and NHBE cells as well as in the lungs of miceintratracheally inoculated ... 4 signaling pathway acts as a positive regulator for TLR2 induction by bacteria via a dual mechanism involving functional cooperation with NF-kappaB and MAPK phosphatase 1-dependent neg-ative ... Biotechnology (Santa Cruz, CA, USA). An antibody against p65 was purchased from Santa CruzBiotechnology. Monoclonal antibody against b-actin waspurchased from Sigma (St Louis, MO, USA).Immunoﬂuorescent...

Tài liệu Báo cáo khoa học: The role of antioxidants in the cytotoxicity of chemotherapeutic drugs pptx

... osmoregulation and sporulation. Isola-tion and characterization of yeast mutants blocked at various sta-ges of transport pathways are an invaluable method forunravelling the molecular details of vacuolar ... binding of apoE. (iii) ABCA1,syntaxin-13 and ﬂotillin-1 operate in both loading conditions with different response rates and downstream signalling involving an inward rectifying K+-channel along ... development of vascular diseases. On the contraryto these adipocytokines, adiponectin, an adipose-tissue-speciﬁc,collagen-like protein, has been noted as an important antiathero-genic and antidiabetic...

Tài liệu Báo cáo khoa học: "The Nature of Affixing in Written English" docx

... formulate an operational defini- tion of 'affix'. The words in Class II, typified by REPLACE, have the property that the internal-consonant string is an admissible initial-consonant ... examine the Class IV words. The Class IV words are distinguished by the property that the internal consonant string is neither an admissible initial- nor an admissible final-consonant ... pass, and it is fairly clear that programs will have to operate at well above the 99% level of accuracy if they are to attain any degree of general use. The attainment of such precision, and...

Tài liệu Báo cáo khoa học: "The Role Of Focussing in Interpretation of Pronouns " pdf

... choice rather than by search ~lsing general inference. The paper also indicates what additional requirements are needed for a full treatment of pronominal anphora. These include use of a representation ... research done at the Artificial Intelligence Laboratory of the Massachusetts Institute of Technology. Support for the laboratory's artificial intelligence research is provided in part ... combinatorial search or which will insure only one search path is taken. In addition, Winograd and Lockman are aware of pronopn phenomena which cannot be treated strictly by inference, as shown...

Tài liệu Báo cáo khoa học: "The Role of Initiative in Tutorial Dialogue" pdf

... of the authors of the pa-per and another human annotator (not a projectmember) corrected the automatic annotations. The annotators had a reference manual and trainedon trial sessions of the ... dialogues. To test inter-annotator reliability, the author and external anno-tator labeled the same 757 examples taken fromnon-training data; the resulting inter-annotator re-liability as ... (1986) theory of discourse. What itmeans to take the lead in developing the agents'plan depends on the plan representation but infor-mally can refer to adding or taking away actionsfrom the...

Báo cáo khoa học: The product chain length determination mechanism of type II geranylgeranyl diphosphate synthase requires subunit interaction pptx

... ananatis, as the template, the crtE geneencoding GGPS was ampliﬁed using PCR with KOD DNApolymerase (Toyobo, Osaka, Japan) and the primers: PaGGPS-Fw, 5¢-AAGAAACATATGACGGTCTGCGCAAAAAAACACG-3¢, ... such asgeranyl diphosphate synthase (GPS) and longer-chainprenyl diphosphate synthases.ResultsRefolding and puriﬁcation of recombinantP. ananatis GGPSP. ananatis GGPS and the mutant enzymes ... sequences in FARM of P. ananatis GGPS and G. stearothermophilus FPS. (A) Partial amino acid sequencesaround FARM of P. ananatis GGPS and mutated enzymes are aligned. The deleted positions are shaded....

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