Báo cáo khoa học: Structure, epitope mapping, and docking simulation of a gibberellin mimic peptide as a peptidyl mimotope for a hydrophobic ligand pot

Báo cáo khoa học: Functional ﬁne-mapping and molecular modeling of a conserved loop epitope of the measles virus hemagglutinin protein pdf

... 1.35-mMphosphatase substrate(SIGMA 104Ò, Sigma-Aldrich, Bornem, Belgium) solutionwas added and the absorbance was measured after 30 and 60 min at 405 nm on a SPECTRAmax PLUS384microplatereader system ... coating,the HNE peptide was at least partially oxidized and thesignal of the reduced species increased as a result of oxidation.Identification of the active isoformBecause of disulfide scrambling HPLC-purified ... immobilizedreporter peptide was recorded by sensorgrams allowing anassociation time of 300 s and a dissociation of 180 s under a constant flow rate of 20 lLÆmin)1at 25 °C. The sensorgramprofile of each run was...

Báo cáo khoa học "ADIABATIC TEMPERATURE RISE AND REACTION RATE OF MASS STRUCTURE IN LOTTE CENTER HANOI PROJECT " pptx

... value analyzed, the period of radiation was somewhat shorter than the value analyzed. This can be assumed that the amount of rise temperature and reaction velocity are accelerated by accumulated ... be decreased and the peak point of hydration heat for the total amount of binder would be increased as increasing the quantity of cement. Therefore, the amount of cement was designed as the ... Plan of concrete placing and curing management for mat foundation Mat foundation constructed in this field consists of 14 layers of mass as if stair shape as seen in Figure 6. And concrete was...

Tài liệu Báo cáo khoa học: X-ray crystallographic and NMR studies of pantothenate synthetase provide insights into the mechanism of homotropic inhibition by pantoate docx

... therelaxation data, and N. Srinivasan and S. Yamunadevi for their help with modeling and analyzing the A. thaliana PS protein.References1 Umbarger E & Brown B (1958) Isoleucine and valinemetabolism ... 4A ˚ and 5.5 A ˚,whereas the backbone Ca atom moves by only 0.9 A ˚ and  0.5 A ˚, respectively. The backbone Caatom of Asp35 moves by as much as 1.62 A ˚ and Fig. 6. An expanded view of the ... Asp for E. coli PS and Glu for M. tuberculosis and A. thaliana PS). As compared with the substrate-free form, the imidazole rings of His34 in the A- chain and B-chain of nPS move by  4A ˚and...

Tài liệu Báo cáo khoa học: Processing, catalytic activity and crystal structures of kumamolisin-As with an engineered active site pptx

... AshidaM, Minakata H, Oyama H, Oda K, Nishino T &Nakayama T (2004) Crystallographic and biochemicalinvestigations of kumamolisin -As, a serine-carboxyl pep-tidase with collagenase activity. ... enhance-ment of the 57-kDa band with a concomitant diminu-tion of the 38-kDa and 19-kDa bands, as analyzed bySDS ⁄ PAGE (Fig. 3A) . The 38-kDa and 19-kDa bandsdid not disappear completely after ... activesites of these enzymes contain a unique catalytic triad,Ser-Glu-Asp, in place of the canonical Ser-His-Asptriad of the classical serine peptidases. In the lattercase, the Ser and His residues act...

Báo cáo khoa học: Alternative splicing: good and bad effects of translationally silent substitutions pdf

... altered isoformproportions, activation of a control mechanism such as nonsense-mediated decay, as well as the creation orloss of splicing variants. As this process has a signiﬁ-cant impact on ... codon information, for an amino acid.Translationally silent variations thataffect splicing and diseaseClinical studies identifying aberrant splicing mutationsare of great importance for genetic ... recently an expensive task) and in the underappre-ciated belief that sequence variations far away mayaffect far away splicing signals. With increased aware-ness we predict that this will change...

Báo cáo khoa học: Protein–protein interactions and selection: generation of molecule-binding proteins on the basis of tertiary structural information potx

... meth-odology, and recent structural visualization of candi-date proteins by X-ray or NMR structural analyses and the construction of artificial libraries allow con-structive selection and functionalization ... VHH and NCS (Fig. 2A) ;grafting of the CDR 3 loop of antilysozyme VHHfunctionalized NCS without denaturation, althoughthe thermal stability was decreased and the affinity for lysozyme was weaker ... into a loop structure of TOP that was identified by moleculardynamics simulation as a suitable location withoutdenaturation (Fig. 2B). CDR-grafted TOP had affinity for CD4 receptor, and was not...

Báo cáo khoa học: Substrate speciﬁcity and transport mode of the proton-dependent amino acid transporter mPAT2 potx

... but also osmolytes such as sarcosine and betaine, and theD-enantiomers of serine and alanine. Theapparent afﬁnities of PAT1 substrates are mainly in therange of 2–15 mM[1,2,4,6,9]. As shown ... extracellular pH, by determining theapparent Kmvalues of glycine and a lanine at pH values of pH 5.5–8.5 (Fig. 4) and a broad range of membranepotentials. At hyperpolarized membrane potentials Kmvalues ... reversal potential however, wassmaller than t heoretically predicted for cotransport of a Fig. 4. Subs trate apparent Kmvalues as a function of the extracellularpH. (A, B) Membrane potential...

Báo cáo khoa học: Dual mitochondrial localization and different roles of the reversible reaction of mammalian ferrochelatase ppt

... phospho-ferrochelatase of S13 0A and S30 3A was decreased, and the band was not detected in the double mutantS13 0A and S30 3A, indicating that ferrochelatase wasphosphorylated at positions 130 and 303. The reaction of ... S13 0A, S30 3A and S33 0A were prepared: inthe ﬁrst round of PCR, human ferrochelatase [43] was used as a template. Primer pairs used were primer A (5¢-AAGAATTCGGTGCAAAACCTCAAGT-3¢) as forward ... pcDNA-HA-FECH, and incubated for 23 h. They were then ﬁxed, per-meabilized and reacted simultaneously with anti-ATP synthase and anti-HA sera to demonstrate localization of ATP synthase and ferrochela-tase....

Báo cáo khoa học: Sequences, geographic variations and molecular phylogeny of venom phospholipases and threeﬁnger toxins of eastern India Bungarus fasciatus and kinetic analyses of its Pro31 phospholipases A2 docx

... 5¢-ATGAAAACTCTGCTGCTGACCTTG-3¢ and primer 2 was 5¢-CTCAAGGAAWTTAGSCAC TCRKAGAG-3¢. For ampliﬁcation of PLAs, the primers 3 and 4 used were 5¢-GCAGTTTGTGTCTCCCTCTTAGGA-3¢ and 5¢-CACAGTCCTTGAGCTGAAGCTTCTC-3¢. In addition, ... the PCR reactions. The Ala49 mutants arerather unique among the elapid venom PLAs, and mutations of Asp49Ala, Tyr28Asn and Gly30Asp attheir catalytic Ca2+binding sites [5] presumably abol-ish ... venom phospholipases and threeﬁnger toxins of eastern India Bungarus fasciatus and kinetic analyses of its Pro31 phospholipases A 2Inn-Ho Tsai1, Hsin-Yu Tsai1, Archita Saha2 and Antony Gomes21...

Tài liệu Báo cáo khoa học: Structure and function of active chromatin and DNase I hypersensitive sites pdf

... complexthan in T cells, with several peaks of nuclease hyper-sensitivity [85]. Mast cells express GATA-2 as well as NFAT and AP-1, and GATA-2 initiates the formation of an additional discrete GATA-2 ... elongating Pol II. Inaddition to yeast SAGA and NuA4, the mammalianHAT HBO1 is also potentially able to be recruited to and to acetylate H4 on K5, K8 and K12, as well as H3 throughout active gene coding ... In cases such as this, DHSsare highly dynamic and can form and disappear inparallel with the induction and depletion of speciﬁcfactors. As also discussed in detail elsewhere in anaccompanying...

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