Báo cáo khoa học: Coenzyme A affects ﬁreﬂy luciferase lumine

Báo cáo khoa học: Coenzyme A affects ﬁreﬂy luciferase luminescence because it acts as a substrate and not as an allosteric effector pot

... A affects firefly luciferase luminescence because it acts as a substrate and not as an allosteric effector Hugo Fraga 1,2 , Diogo Fernandes 1,2 , Rui Fontes 2 and Joaquim C. G. Esteves da Silva 1 1 ... structural similarity between firefly luciferase and acyl-CoA synthetases, our achievement is not as strange as it seems to be and supports t...

Ngày tải lên : 16/03/2014, 23:20

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Báo cáo khoa học: New application of ﬁreﬂy luciferase ) it can catalyze the enantioselective thioester formation of 2-arylpropanoic acid docx

... basic region. Thioesterification acitivity of L. cruciata and P. pyralis luciferases From the above experiments (Table 1, entries 1–3), it was known that L. cruciata and P. pyralis luciferases also have an ability to catalyze ... also has the ability to form thioesters of long-chain fatty acids. This report indica- ted that firefly luciferase also has an acyl-CoA synthetase act...

Ngày tải lên : 07/03/2014, 10:20

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Báo cáo khoa học: Interaction of the general transcription factor TnrA with the PII-like protein GlnK and glutamine synthetase in Bacillus subtilis potx

... TnrA and disable its activity. In cells grown with an energetically unfavorable nitrogen source such as nitrate, TnrA is fully membrane-bound via a complex of AmtB and GlnK, which are the trans- membrane ... 1781 was incubated with various effector molecules, and the mixture was used as an analyte in SPR analysis. ATP and 2-oxoglutarate are known to be the primary effec- t...

Ngày tải lên : 06/03/2014, 00:21

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Báo cáo khoa học: Characterization of HbpR binding by site-directed mutagenesis of its DNA-binding site and by deletion of the effector domain pot

... displayed a luciferase activity of about 500-fold lower, albeit with a slight increase at 2-HBP concentrations of 1, 2, 10 and 20 lm (Fig. 5C). As we can assume that the expression level of CBP–DA- HbpR ... positions. That the rigidity of the binding region was not inﬂuenced by the change from four AT to four or six GC, was shown by mutants 8 and 9 (each with 3· GC and ﬁve mut...

Ngày tải lên : 07/03/2014, 17:20

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Báo cáo khoa học: Secondary structure assignment of mouse SOCS3 by NMR deﬁnes the domain boundaries and identiﬁes an unstructured insertion in the SH2 domain pdf

... 77–78. 28 Sasaki A, Inagaki-Ohara K, Yoshida T, Yamanaka A, Sasaki M, Yasukawa H, Koromilas AE & Yoshimura A (2003) The N-terminal truncated isoform of SOCS3 translated from an alternative initiation ... D. Scrofani 2 , Manuel Baca 1, † and Raymond S. Norton 1 1 Walter and Eliza Hall Institute, Parkville, Victoria, Australia 2 Amrad Corporation Ltd, Richmond, Victoria, Australi...

Ngày tải lên : 16/03/2014, 14:20

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Báo cáo khoa học: Internalization of the human CRF receptor 1 is independent of classical phosphorylation sites and of b-arrestin 1 recruitment potx

... on the assumption that receptor and ligand are endocytosed together and that the intracellular receptor–ligand complex can be determined by measuring intracellular radioactive labelled ligand. Following 125 I-labelled ... 0.4 M sucrose for 20 min and this concentration was maintained during radioligand incu bation. Data were analysed using Graph- Pad PRISM software. Results Visualizatio...

Ngày tải lên : 30/03/2014, 15:20

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Tài liệu Báo cáo khoa học: Four divergent Arabidopsis ethylene-responsive element-binding factor domains bind to a target DNA motif with a universal CG step core recognition and different ﬂanking bases preference pptx

... were aligned computationally and the appearance of a base at each position in a motif was presented as a percentage frequency of all four kinds of base. The base with a frequency higher than ... cold-, drought- and ABA-regulated gene expression. Plant Mol Biol 24, 701–713. 15 Prabakaran P, An J, Gromiha M, Selvaraj S, Uedaira H, Kono H & Sarai A (2001) Thermodynamic da...

Ngày tải lên : 18/02/2014, 13:20

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Tài liệu Báo cáo khoa học: Progress for dengue virus diseases Towards the NS2B–NS3pro inhibition for a therapeutic-based approach ppt

... years as a result of the expansion of the Aedes aegypti mosquito to different geographic areas, and DHF has spread from South East Asia to the Western Paciﬁc and the Americas. A substantial ... multifunctional protein with an N-terminal protease domain (NS3pro) (1–180), an RNA triphosphatase, an RNA helicase and an RNA- stimulated NTPase domain in the C-terminal region [...

Ngày tải lên : 19/02/2014, 00:20

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Tài liệu Báo cáo khoa học: Characterization of the interaction between the plasma membrane H+-ATPase of Arabidopsis thaliana and a novel interactor (PPI1) doc

... Characterization of the interaction between the plasma membrane H + -ATPase of Arabidopsis thaliana and a novel interactor (PPI1) Corrado Viotti, Laura Luoni, Piero Morandini and Maria Ida De ... & Rasi-Caldogno F (1998) Fusicoccin binding to its plasma membrane receptor and the activation of the plasma membrane H + -ATPase. IV. Fusicoccin induces the association between the plasm...

Ngày tải lên : 19/02/2014, 07:20

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Tài liệu Báo cáo khoa học: Characterization of promoter 3 of the human thromboxane A2 receptor gene A functional AP-1 and octamer motif are required for basal promoter activity docx

... HEL92.1.7 (A and B) and HEK293 (C and D) cells. Firefly and renilla luciferase activity was assayed 48 h post-transfection; results are presented as mean firefly relative to renilla luciferase activity, expressed ... Thereafter, firefly and renilla luciferase activity was assayed; results are presented as mean firefly relative to renilla luciferase activity, expres...

Ngày tải lên : 19/02/2014, 16:20

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