Báo cáo khoa học: Interaction of the C1 complex of Complement with sulfated polysaccharide and DNA probed by single molecule ﬂuorescence microscopy pdf

... Interaction of the C1 complex of Complement with sulfated polysaccharide and DNA probed by single molecule ﬂuorescence microscopy Be´range`re Tissot1,Re´gis Daniel1 and Christophe ... bind to DNA strands in the absence of C1q (datanot shown). Therefore the binding of C1r is a directconsequence of the presence of C1q on DNA strands.Fig. 2. Molecular combing of the T4 DNA on ... proved that the binding of C1r to C1q isinhibited by fucoidan, consistent with the interaction of the polysaccharide with the hinge region of the collagen-likeregion of C1q.DiscussionC1q can...

Tài liệu Báo cáo khoa học: nsights into the reaction mechanism of glycosyl hydrolase family 49 Site-directed mutagenesis and substrate preference of isopullulanase doc

... polysaccharide, pullulan.Comparison of the active sites of the model of IPU and Dex49A The active site structures of t he model of IPU (Fig. 4A) and Dex49A (Fig. 4B) were compared. The three Asp residues,Asp353, Asp372 and Asp373, ... as the substrate. The anomericforms of t he hydrolysate are equilibrated immediately by the addition of ammonium hydroxide, and the change in the optical rotation was compared with those of ... activating the nucleophilic water m olecule) in the ﬁrst step of the reaction.A carbonium ion intermediate subsequently forms, and isfurther attacked by the water molecule. The study of site-directed...

Tài liệu Báo cáo khoa học: "Methods for the Qualitative Evaluation of Lexical Association Measures" doc

... (Figures 3 and 4), we find: (i) Examination of 50% of the datain the SLs leads to identification of between 75%(AdjN) and 80% (PNV) of the TPs. (ii) For the first 40% of the SLs, and lead to the worstresults, ... fre-quency) and the weak measures ( and ).In the second half of the data in the SLs, how-ever, there is virtually no difference between ,, and the other measures, with the exception of mere co-occurrence ... Evaluation of Association Measures2.1 State -of- the- artA standard procedure for the evaluation of AMs ismanual judgment of the -best candidates identi-fied in a particular corpus by the measure...

Báo cáo khóa học: Insight into the activation mechanism of Bordetella pertussis adenylate cyclase by calmodulin using ﬂuorescence spectroscopy pptx

... ﬂuorescence heterogeneity with speciﬁc couplingFig. 5. Binding of Ant-dATP to AC (d) and AC–CaM (m). The degree of binding of the ligand was calculated from the value of the ampli-tude of the ... the uncomplexed AC and the AC–CaM complex. Dynamics of the AC catalytic domain as probed by acrylodanTaking advantage of the absence of Cys residues in the native AC protein, the insertion of a ... explain the mechanism of CaM activation of the latter. Crystallization and resolution of the 3D structures of AC and AC–CaM,which are still not available, and labeling of the AC protein atother...

Báo cáo khoa học: Bacitracin inhibits the reductive activity of protein disulﬁde isomerase by disulﬁde bond formation with free cysteines in the substrate-binding domain pptx

... 10 mm). The structural identity of the isolated analogs was con-ﬁrmed by MALDI-TOF ⁄ TOF MS, with a 4800 MALDI-TOF ⁄ TOF Analyzer (AB SCIEX, Framingham, MA,USA). MALDI-TOF and TOF ⁄ TOF MS spectra ... Cys345 and the thiol form of bacitracin A. The thiol form of the thiazoline ring of bacitracin A and Cys345are shown as chemical structures, the rest of bacitracin A as ‘Bacitracin A’, and all other ... the gel matrix and analyzed by MALDI-TOF ⁄TOF MS. The PDI–bacitracin was characterized by a combination of in-source decay and CID-TOF ⁄ TOF MS, based on the method described by Kleffmann et...

Báo cáo khoa học: Intrabodies against the EVH1 domain of Wiskott–Aldrich syndrome protein inhibit T cell receptor signaling in transgenic mice T cells potx

... consisting of VH and VLregions with ⁄ without the VHleadersequence at the N-terminal and with ⁄ without the CL(j) region behind the VLregion. None of the con-structs contained the KDEL ... j and IgG2b ⁄ j, respectively. The appropriate cDNA fragments of the VH and VLregions were then generated by RT-PCR. A compar-ison of the VH and VLamino-acid sequences of clones 18 and ... constructs with and without the N-terminal leader signal sequence of the VHregion and with and without the CL(j) region following the VLregion, which are described as HL, SHL, HL-CL and SHL-CL...

Báo cáo khoa học: Principles behind the multifarious control of signal transduction ERK phosphorylation and kinase/phosphatase control potx

... controlled by the kinases or phosphatasesin the system, and whether they are controlled differ-entially by some or all of them. On the basis of the antagonistic action of the kinases and phosphatasesone ... to how these time patterns are controlled by the kinases and the phosphatases.In order to examine how the second kinase in the cascade determines the time dependence of the activity of the third ... as the total area under the Table 1. Control of any out of four characteristics of the time dependent signal by any of the molecular processes in the cascade. The signal is taken to reside in the...

Báo cáo khoa học: "Search in the Lost Sense of “Query”: Question Formulation in Web Search Queries and its Temporal Change" pptx

... compute a linear regression of Q-level across 12 months, conduct a hypothesistest (with the null hypothesis being the slope of the regression equal to zero), and report the P -value fortwo-tailed ... for by Q-queries. Weplot this against b’s activity level, measured by the number of queries issued by b in a month. We binnedusers by their activity levels on the log2-scale and compute the ... e.g., Yi and Maghoul (2009) and Wen et al. (2002). We focus not on clusteringbut on understanding the expression of intent.3 MethodWe address the main thesis of the work by retrospec-tively studying...

Báo cáo khoa học: Studies on the regulatory properties of the pterin cofactor and dopamine bound at the active site of human phenylalanine hydroxylase pptx

... conformation and hydrogen bondingnetwork of the pterin cofactor in the three structures.Superpositions of the binary and ternary complexes of hPAH onto the crystal structure of the ligand-free ... changes of the pro-tomer resulting in a displacement of the cofactor bound at the active site by 2.6 A˚(mean distance) in the direction of the iron and Glu286 which causes a loss of the stabilizinghydrogen ... Similarly, the coordinates for the structure of the binary complex with dopamine, i.e.hPAH-Fe(III)Ædopamine (PDB id code 5PAH) [17] deﬁne the position and orientation of the inhibitor and the interaction...

Báo cáo khoa học: Alterations in the photoactivation pathway of rhodopsin mutants associated with retinitis pigmentosa potx

... like the newly reported G90V adRP mutation [19]. The G51A,G51V and G89D mutants were studied in the context of the folding and packing of the TM domain together with other adRP mutations in the ... dramatically inﬂuenced by pH.Rate of Meta II decay as measured by retinalrelease The rate of retinal release, which parallels the Meta IIdecay of the protein in the case of the WT under the condi-tions ... consistent with the results obtained in DMsolution. The altered band of these mutants, in DM solution,had less intensity than the corresponding band formedin the case of the G51V single mutant....

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