Báo cáo khoa học: Characterization of D-amino-acid-containing excitatory conotoxins and redeﬁnition of the I-conotoxin superfamily pot

... wells 3 and 4. At the same time, the activity of the muscle was recorded with apair of electrodes: one electrode was located in the middle, and the other at one end, of the trough. Each pair of recording ... nerve, and epimerization of the single amino acid at the third posi-tion from the C-terminus attenuates the potency of r11a and r11b, but notthat of r11c. Furthermore, r11c (but neither r11a ... to as the I-conotoxin superfamily should nowbe divided; a potential connection between the identity of the gene superfamily to which the conotoxinbelongs, and the presence or absence of a d-aminoacid...

Tài liệu Báo cáo khoa học: TEC family kinases in health and disease – loss-of-function of BTK and ITK and the gain-of-function fusions ITK–SYK and BTK–SYK pptx

... identify individualswith mild disease, thereby providing the missing data. The phenotype of XLA and the potential of BTK and ITK inhibitors The outcome of defective BTK signaling in humanshas ... posi-tioned in the connecting loops. Approximately one-third of all mutations in the BTK gene are missense and some of these reduce the stability of the protein.This is exempliﬁed by mutations in the ... 2001–2010 ª 2011 The Authors Journal compilation ª 2011 FEBSMINIREVIEWTEC family kinases in health and disease – loss -of- function of BTK and ITK and the gain -of- function fusions ITK–SYK and BTK–SYKAlamdar...

Tài liệu Báo cáo khoa học: Competition between innate multidrug resistance and intracellular binding of rhodamine dyes pdf

... comparison of rhodamine uptake, in the presence and the absence of uncouplers, allows the estimation of the effect of intracellular binding on the MDR phenomenon. The data detailed obtained in the present ... with the accumulation of dyes, and probably certain drugs,in the cytoplasm. On the other hand, high-afﬁnitybinding of these agents to intracellular receptors over-whelms the low levels of ABC ... intracellular quenching of the dyes; and (c) it measures the total dye amount associatedwith the cells, including the dye adsorbed to the sur-face of the cells.To avoid complication of the uptake studies...

Tài liệu Báo cáo khoa học: A facile method for expression and puriﬁcation of the Alzheimer’s disease-associated amyloid b-peptide pdf

... significantlyincreases the cost of the purification, and the require-ment for HPLC increases the length and complexity of the purification procedure. Moreover, as with the studies by Lee et al. [21] and Subramanian ... Subramanian and Shree[42], there was no rigorous assessment of the purity of the product or the correctness of the sequence. In con-trast, the purification protocol that we have developedis quick and ... understanding of the structure of both the Ab monomer and its various assemblies could help in the design of new therapeutic strategies targeted atpreventing the formation or ameliorating the activityof...

Báo cáo khoa học: Human telomeric G-quadruplex: thermodynamic and kinetic studies of telomeric quadruplex stability potx

... [16,19,20,23]. The energy of folding these vari-ous forms and the energetic cost of converting oneform to another is of fundamental importance forunderstanding telomere biology and the interactions of small ... fullyunderstand such allosteric effects, the energetics of the underlying conformational transitions in quadruplexstructures must be determined. The thermodynamics and kinetics of the transition of the ... practical problems. There are worrisome reportsdocumenting that the lengths of the pre- and post-transition baselines selected and used in data analysisdirectly affect the values of the thermodynamic...

Báo cáo khoa học: A study on genomic distribution and sequence features of human long inverted repeats reveals species-speciﬁc intronic inverted repeats pptx

... the effect of the length of these regions on the distribution. The intronic and intergenic regions were then classiﬁedon the basis of their lengths. Within each of the lengthgroups, the numbers of ... genome, the frequency of their complementaryrepeats (TG)n and (CT)nis much lower [37].Therefore, the presence of the TG ⁄ CA-rich and TC ⁄ GA-rich LIRs is not a result of the enrichment of (CA)n and ... (NPS-Sep-tin) and eight in genes encoding POTE. The genesbelong to gene families and their duplication is coupledwith the spread of the LIRs inside the gene. The remaining LIRs aside from the above...

Báo cáo khoa học: "Why Are They Excited? Identifying and Explaining Spikes in Blog Mood Levels" potx

... least one of the overused words and is dated within the peaking interval or the day be-fore the beginning of the peak. The hits are rankedbased on the number of overused terms containedin the headline.5 ... to tag their entries with highly individual (and personal) features. Users of LiveJournal, one of the largest weblog communities, have the option of reporting their mood at the time of the post;users ... and DW (date) is a day -of- the- week function that returns 0, . . . , 6 dependingon the date argument. The level of a given mood is changed withina one-hour interval of a day, if the ratio of...

Tài liệu Báo cáo khoa học: Characterization of 1H NMR detectable mobile lipids in cells from human adenocarcinomas doc

... lymphocytes and developing cells. On the other hand, a correlation of the intensity of these signals with metabolic parame-ters is not straightforward. Some studies noted that the signal intensity of ... Fig. 3A) to the spectralﬁtting of samples without ML signals. The presence of these peaks was paralleled by the existence of lipidcross peaks in the 2D spectra (Table 1 and Fig. 3B). The cross ... guarantee the presence of a perfect Gauss-ian shape. The second hypothesis necessary to performStudent’s t-test requires that the variance of the two samplesis equal. By estimating the variance...

Tài liệu Báo cáo khoa học: Characterization of chitinase-like proteins (Cg-Clp1 and Cg-Clp2) involved in immune defence of the mollusc Crassostrea gigas docx

... characterizedby the degeneration of larval tissues, such as the velum and the foot, and the remodelling of larval tissues toproduce adult tissues (i.e. the development of the gills and the production of ... sequence of about 90 amino acids of unknown function. The high degree of identity of the Cg-Clp1 and Cg-Clp2 Glyco_18 domains (84% iden-tity) argues for a conservation of the tertiary structure and ... con-servation of this class of proteins at both the structural and functional levels. Given the multiplicity of CLPsin humans and insects [14], we hypothesized thathomologues of the previously characterized...

Tài liệu Báo cáo khoa học: Characterization of human deoxyribonuclease I gene (DNASE1) promoters reveals the utilization of two transcription-starting exons and the involvement of Sp1 in its transcriptional regulation ppt

... and quantitativereal-time RT-PCR, the locations of the primers DN)105 and DN)9used in ChIP assays, the locations of the primers DN+232 and DN+254 utilized in 5¢-RACE, and the locations of the ... fragments F and I. The number at the right of each RT-PCR product represents the length of the prod-uct. The scheme also shows the location of the reverse primerDN+89 used in another starting ... numbers over the diagrams indicate the position of the corresponding nucleotides relative to the translation start site, and the numbersin parentheses below the diagram show the position of the corresponding...

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