Báo cáo khoa học: Correlation between conformational stability of the ternary enzyme–substrate complex and domain closure of 3-phosphoglycerate kinase potx

... Substrate-assisted domain domain cooperativityFEBS Journal 272 (2005) 1867–1885 ª 2005 FEBS 1885 Correlation between conformational stability of the ternary enzyme–substrate complex and domain closure of 3-phosphoglycerate ... estimated. These correlatewell with the increased stability of the ternary complex vs. the binary ones.This increased stability of the ternary complex maysimply be due to simultaneous formation of the ... route of transmission of the substrate-induced conformational effects from one domain to the other. The largest stability is characteristic of the native ternary complex and is abolished in the...

Báo cáo khoa học: "Correlation between ROUGE and Human Evaluation of Extractive Meeting Summaries" pptx

... 5% of the DAs and 16% of the words of the entiremeeting. Thus, in total we have 36 human summaries and 24 system summaries on the 6 test meetings, on which the correlation between ROUGE and ... and spoken documents, es-pecially in the meeting domain, for example, the pres-ence of disfluencies and multiple speakers, and the lack of structure in spontaneous utterances. The question of whether ... investigate the correlation between ROUGE and human evaluation of extractive meetingsummaries and focus on two issues specific to the meet-ing domain: disfluencies and multiple speakers. Bothhuman and...

Báo cáo khoa học: Correlation between functional and structural changes of reduced and oxidized trout hemoglobins I and IV at different pHs doc

... the interactions between the heme and the surrounding globin,being inﬂuenced by the attached ligand and thereby by the spin state of the iron atom [11]. According to Perutz et al.[18], the region ... in the Q0band[25,26]. The splitting of these bands has been regarded as alowering of the heme symmetry in HbA-CO [17,27], due tononperpendicular iron–ligand bond above the xy plane of the ... that the Soret band in the T-form is slightly blue shifted and higher in intensity than the R-form, the measured effect in the Soret band is likelydue to the superimposition of deoxygenation and...

Báo cáo khoa học: Arginine-induced conformational change in the c-ring ⁄a-subunit interface of ATP synthase ppt

... pocket of the I. tartaricus c-ring. Harmonic restraints were placed on the backbone atoms of the c-ring, with the intention that only the residues of the a subunit and the side chains of the c-ring ... to the outer helix of the c-ring at a dis-tance between helix axes of 12 A˚. The longitudinal rotation of the model helix was such that the side chain of aR226pointed towards and into the ... paircontained the codon for the desired mutation, the sequence of the other was derived from the wild-type. The presence of the mutant codons was conﬁrmed by automatedsequencing of the cloned DNA...

Tài liệu Báo cáo khoa học: Relationships between structure, function and stability for pyridoxal 5¢-phosphate-dependent starch phosphorylase from Corynebacterium callunaeas revealed by reversible cofactor dissociation studies doc

... in the corresponding pH-rate proﬁles. The pKavalues of the cofactor phosphate in unligandedEcMalP and the EcMalP–arsenate complex are 5.6 [26] and 6 .7 [27], respectively. The pKa of the ... in the presence of 5 mMpotassium phosphate and potassium sulphate.Reconstitutions with PLP of apo-CcStP and mutantsthereof, and characterization of the wild-typeholo-enzymeIncubation of ... thermostabilization byphosphate has been examined. The role of the cofactor5¢-phosphate group in the induction of stability and stabil-ization of the CcStP dimer has been studied. Subunitcomplementation...

Báo cáo khoa học: Relationship between functional activity and protein stability in the presence of all classes of stabilizing osmolytes ppt

... and enzyme–product complexes [29]. On the other hand,kcat⁄ Kmis an apparent second-order rate constant thatrefers to the properties and the reaction of the freeenzyme and free substrate [29]. We have therefore ... of RNase-A in the presence of these amino acids and amino acid derivatives, and values of DGD°, measuredin triplicate, are given in Table 1. The effect of polyols on the kinetic parameters (Km and ... preferential hydration of the protein domain is the driving force of protein stabiliza-tion or folding [3,4]. Furthermore, the effect of osmolytes on the functional activity of an enzyme hasalso...

Tài liệu Báo cáo khoa học: Interaction between very-KIND Ras guanine exchange factor and microtubule-associated protein 2, and its role in dendrite growth – structure and function of the second kinase noncatalytic C-lobe domain docx

... localized to the N-terminal region, and theirspeciﬁc functional domains are located in the C-termi-nal region. The C-lobe of protein kinases mediates the interaction with activators, substrates and ... central domain of MAP2contain the v-KIND binding core moduleMAP2 consists of three main structural domains: the cAMP-dependent protein kinase regulatory subunitRII binding domain, the central domain ... within the MAP2 center region of the CD. (A) MAP2a was subdivided into ﬁvefragments [the cAMP-dependent protein kinase regulatory subunit RII binding domain (RII), the central domain (CD)1-3 and the...

Tài liệu Báo cáo khoa học: Competition between innate multidrug resistance and intracellular binding of rhodamine dyes pdf

... comparison of rhodamine uptake, in the presence and the absence of uncouplers, allows the estimation of the effect of intracellular binding on the MDR phenomenon. The data detailed obtained in the present ... with the accumulation of dyes, and probably certain drugs,in the cytoplasm. On the other hand, high-afﬁnitybinding of these agents to intracellular receptors over-whelms the low levels of ABC ... intracellular quenching of the dyes; and (c) it measures the total dye amount associatedwith the cells, including the dye adsorbed to the sur-face of the cells.To avoid complication of the uptake studies...

Tài liệu Báo cáo khoa học: Interactions between M proteins of Streptococcus pyogenes and glycosaminoglycans promote bacterial adhesion to host cells pdf

... involved in the interaction as the binding of 125I-labelled DS was reduced to isogenicderivatives of the AP1 strain lacking either of these surfaceproteins (Fig. 2). The signiﬁcance of the M1 protein ... electrostatic interactions between the negatively charged sulfate groups of the GAGchains and positively charged regions of the ligand. Typi-cally, the heparin-binding domains of known GAG-bindingproteins ... ﬁbrinogento the NH2-terminal half (A–B3), for IgG to the central S domain, and for human serum albumin to the C-repeats(C1–C3) [31]. None of these protein ligands was able toinhibit the binding of 125I-labelled...

Báo cáo khoa học: Interactions between coenzyme B12 analogs and adenosylcobalamin-dependent glutamate mutase from Clostridium tetanomorphum pot

... energy of the cofactoris sensitive to changes in the pKa of the lower axialbase [3]. This has led to speculation that proteinsmight modulate the pKa of the histidine via the hydrogen bond between ... site [18,19]. The correlation between the distortion of the adenosyl group and cleavage of the cobalt–carbon bond is still not clear.Although the precise mechanism remains obscure, the results ... volume of 100 lL containing 2 mm MgCl2,40mml-glutamate and 50 mm Tris buffer, pH 8.5. The Km and kcatfor Ado-Cbi were determined in the presence of 10, 25, 50, 75 and 120 lm cofactor, and the...

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