Báo cáo khoa học: Mapping contacts between regulatory domains of skeletal muscle TnC and TnI by analyses of single-chain chimeras potx

... FEBS Journal 272 (2005) 779–790 ª 2005 FEBS Mapping contacts between regulatory domains of skeletal muscle TnC and TnI by analyses of single-chain chimeras Ana O. Tiroli1,2,*, Ljubica Tasic1,*,†, ... 1.6V[model -TnC( 1–91) TnI( 98–182)] ⁄ V(model -TnC( 1–91) TnI( 98–147)1.3 ± 0.1 MM [TnC( 1–91) TnI( 98–182)] ⁄MM [TnC( 1–91) TnI( 98–147)] 1.3 Contacts between skeletal TnC and TnI regulatory domains A. O. ... available regarding the skeletal muscle TnI TnC complex. With the aim of obtaining structuralinformation regarding speciﬁc contacts between skeletal muscle TnC and TnI regulatory domains, we have constructed...

Tài liệu Báo cáo khoa học: A knowledge-based potential function predicts the speciﬁcity and relative binding energy of RNA-binding proteins ppt

... observed between twoatoms (i,j), of types ti and tj, and Nobs(dij,ti,tj) represents thenumber of atoms of types ti and tjobserved in the structuretraining set, separated by a distance ... structure.Some RRM and KH domains in complex with singlestrand DNA (PDB codes: 2UP1, 1WTB, 1X0F, 1ZZI and 1ZZJ) were also included in the test set because recognition of single stranded RNA and DNA are ... proteins [6,39,40] and by the structure of existing KH domains bound to RNA[6,41–44]. As a consequence of the assumptions of the model, complexes containing two RNA-binding domains were divided...

Báo cáo khoa học: Complex gangliosides are apically sorted in polarized MDCK cells and internalized by clathrin-independent endocytosis doc

... domain of MDCK cells (Fig. 2A, see top of the cell monolayer). To further conﬁrm and quantifythe steady-state distribution of GD3 and GM1gangliosides in apical and basolateral domains of MDCK ... surfacewith the antibody R24 and CTx, speciﬁc for GD3 and GM1, respectively. The binding of R24 antibody and CTx in both apical and basolateral domains was evalu-ated by western blotting. In agreement ... visualized by exposing the plate to iodine vapour. Theposition of GM1 is indicated on the right of the plate. Lipids migrated as multiple bandson the HPTLC plate because of the hetero-geneity of the...

Báo cáo khoa học: An antimicrobial peptide tachyplesin acts as a secondary secretagogue and ampliﬁes lipopolysaccharide-induced hemocyte exocytosis potx

... leakage of hemolymph and the spread of infectious pathogens, while lectins and antimicrobial peptides aggregate and lyse the patho-gens. Hemocyte exocytosis is speciﬁcally induced by LPS, but not by ... signaltransducers by way of nuclear factor (NF)- jB, thusleading to the production of pro-inﬂammatory cyto-kines and the expression of costimulatory molecules onthe cell surface. These cytokines and costimulatorymolecules ... exocytosis of granular hemo-cytes is quite interesting in the context of a comparat-ive examination of the innate immune mechanisms of mammals and horseshoe crabs.The PAMP-induced exocytosis of immune...

Báo cáo khoa học: "Mapping between Compositional Semantic Representations and Lexical Semantic Resources: Towards Accurate Deep Semantic Parsing" docx

... cross-fertilization of systems and resources using differentsemantic representations very difﬁcult.In this paper, we present a machine learning ap-proach towards mapping between deep and shallowsemantic ... seman-189tic predicates and their linguistic behaviour (includ-ing the set of semantic roles, indication of optionalarguments, and their possible value constraints arespeciﬁed by the grammar as ... Predi-cate, Theme, Topic. A treebank of RMRS structures and derivations was generated by using the Prop-Bank corpus. The process of RMRS feature extrac-tion was applied and a new verb dependency treesdataset...

Tài liệu Báo cáo khoa học: Loose interaction between glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate kinase revealed by ﬂuorescence resonance energy transfer–ﬂuorescence lifetime imaging microscopy in living cells doc

... localization and enzymatic activities of GAPDH–citrine and PGK–cerulean. (A) Expression of GAPDH–citrine and PGK–cerulean. Localization of endogenous GAPDH and PGK inHeLa cells was examined by using ... tetramericstructure of GAPDH is schematically shownon the right. The distances between the N-terminal amino acids of subunits O and P,subunits O and Q and subunits O and R areshown (B–D). FLIM analysis of ... the case of cellsamples without coexpression of GAPDH) or from the cytoplasmic area (in the case of cell samples with coexpression of GAPDH) of cells, and are expressed as mean ± standard deviation....

Tài liệu Báo cáo khoa học: Paradoxical interactions between modiﬁers and elastase-2 Patricia Schenker and Antonio Baici docx

... for the inhibition of elastase-2 by a1-PI and interference by sulfated polysaccharides.Fig. S2. Progress curves for the inhibition of elastase-2 by eglin c and interference by sulfated polysaccharides.This ... inhibition and reactivation by the con-comitant action of two modifiers I and X. Plots of the reaction rateas a function of the concentration (mM) of two modifiers. Thekinetic parameters and coefficients ... orpresence of effectors, not to determine absolute valuesfrom their dependence on the concentration of eglin c and a1-PI. The effects of inhibition by a1-PI and eglin c by Ch4S, calculated by regression...

Tài liệu Báo cáo khoa học: Electrostatic contacts in the activator protein-1 coiled coil enhance stability predominantly by decreasing the unfolding rate docx

... mI, mt2 and mfare m-valuesassociated with each of the identiﬁable states of the foldingpathway and relate to the amount of solvent-exposed sur-face area in each of these states, and thus ... GCN4, a yeasthomologue of AP-1, both binding and dissociation of dimers is tightly coupled with folding ⁄ unfolding of theindividual helices, and is well described by a simple two-state model ... for the design and synthesis of therapeutic mimetics.Materials and methodsPeptide synthesis and puriﬁcationPeptides were synthesized by Protein Peptide Research(Fareham, UK) and subsequently...

Tài liệu Báo cáo khoa học: Mapping the functional domains of human transcobalamin using monoclonal antibodies pptx

... tothe standard protocols.Binding of the naturally cleaved TC fragmentsto mAbsExpression of TC in yeast and plants was accompanied by partial cleavage of the protein at several sites by some ... immu-nological reactivity by western blotting and identiﬁed by N-terminal sequencing.Binding of mAbs to synthetic peptides of TCTwo long peptides of 30 residues (PA and PB) were synthes-ized ... absence of S-S bonds and the low number of conserved residuesin this part of the sequence. Only later did the largeN-terminal unit become involved in the binding of Cbl [13,14]. Assembly of two domains...

Tài liệu Báo cáo khoa học: Functional interaction between RNA helicase II⁄Gua and ribosomal protein L4 pptx

... LocalizationFig. 4. Mapping of Gua-binding domain in human RPL4. (A) Schematic representation of wild-type and mutant forms of human RPL4. Theopen bar represents regions conserved between human and mouse ... activity and GTP-stimu-lated RNA foldase activity [17–19]. The presence of both RNA unwinding and RNA folding activities intwo distinct domains of the same protein highly sug-gests a role of Gua ... component of 60S ribosome large subunit. The ATPase activity of Gua is important for this interaction and is also necessary for the func-tion of Gua in the production of both 18S and 28S rRNAs....

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