Báo cáo khoa học: G protein-coupled receptor 30 down-regulates cofactor expression and interferes with the transcriptional activity of glucocorticoid pdf
Báo cáo khoa học: G protein-coupled receptor 30 down-regulates cofactor expression and interferes with the transcriptional activity of glucocorticoid pdf
... sites. The following primers were used for cloning at the pLEGFP-N1vector: GPR30-forward 5¢-TAATAAGTCGACGGGTCTCTTCCT-3¢ and GPR30-reverse 5¢-ATTATTGGATCCTACACGGCACTGC-3¢. V iruses capable of i ... for gene delivery. GPR30 inhibited the growth and proliferation of the cells. They e xpressed glucocorticoid receptor, but not estrogen or progesterone receptor. GPR30down-regulated the expression ... of GPR30 on the regulation of cofactor and g rowthwas due to GPR30 protein expression, we measured the effects of GPR30 fused with EGFP. As a control, we usedHME cells infected with the plasmid-expressing...
- 10
- 389
- 0
Tài liệu Báo cáo khoa học: Agrobacterium tumefaciens type II NADH dehydrogenase Characterization and interactions with bacterial and thylakoid membranes ppt
... FAD-con-taining proteins were observed, confirming the nature of the cofactor [19]. By using the extinction coeffi-cient at 450 nm we calculated that the stoichiometricratio of flavinic cofactor per mole of ... [38] suggested that the presence of an acidic residue (E or D) at the end of the second b sheet of the dinucleotide-binding sitewould confer NADH specificity by providing hydrogenbonding to the ... Hydrogen production.Green algae as a source of energy. Plant Physiol 127,740–748.34 Melis A (2002) Green alga hydrogen production: pro-gress, challenges and prospects. Int J Hydrogen Energy27,...
- 13
- 439
- 0
Báo cáo khoa học: Two conserved domains in regulatory B subunits mediate binding to the A subunit of protein phosphatase 2A pdf
... signi®cant alignment with other B subunits, homology with B/B55 and P R72 familymembers was found when only the B56 binding domainswere used in the a lignment ( Fig. 3). For ASBD 1, the region of ... synthesizedin the reticu locyte lysate (Fig. 1A), suggesting the C subun itpresent in the reticulocyte lysate may contribute t o the formation of heterotrimers [27].To map the region(s) of ... speci®city and subcellular targeting [20,22]. Two additional classes of polypeptides also interact with the AC core of PP2A. Both the small and middle T antigens encoded by polyomavirus and SV40, and the...
- 7
- 550
- 0
Báo cáo khoa học: Hepatocyte growth factor activator (HGFA): molecular structure and interactions with HGFA inhibitor-1 (HAI-1) doc
... hydrogen bonds between P1 Arg and Ser214 and between P3 Gln and Gly216 (Fig. 3A). S1is filled with the P1 Arg, which engages in standardsalt bridge interactions with HGFA Asp189, located at the ... the ranges among close homologs. Additionally, some of these loops (the 140-, 180-, and 220-loop) undergo con-formational rearrangements during the zymogen toenzyme transition, and, together with ... representa-tion of the HGFA–KD1 interaction. Residues on HGFA (green) and KD1 (blue) with an atom within 4.0 A˚ of the other protein (= bindingregion) are indicated. KD1 makes contact with all substrate...
- 8
- 298
- 0
Báo cáo khoa học: Nucleosome positioning in relation to nucleosome spacing and DNA sequence-specific binding of a protein doc
... FEBSurea gels (footprinting) were generated from the Phosphori-mager using the image gauge program of Fuji (Tokyo,Japan). All protections were ascertained by matching the profiles of the lanes with ... precisionwould decrease with distance from the boundary [28].We have analyzed the effect of changing the ionicstrength and the number and spacing of the bindingsites on the protein-generated boundary ... ionic strengths [18]represents the change in the average NRL of the bulkchromatin, and we did not see any disruption of the regular spacing in the presence of R3 (Fig. 3B). There-fore, the small...
- 15
- 299
- 0
Báo cáo khoa học: Superoxide radical-scavenging effects from polymorphonuclear leukocytes and toxicity in human cell lines of newly synthesized organic selenium compounds potx
... p47phoxto the plasma membrane.Thus, we suggest that the O2–-scavenging activity of selenoureas and selenoamides was not due to the inhi-bition of NADPH oxidase in PMNs.We assessed the metabolism ... USA) following the procedure recommended by the manufacturer. The purity of PMNs was > 98% as deter-mined by Randolph’s stain.Assay of effects on scavenging of O2–from PMNsOrganic selenium ... significantlyinhibit xanthine oxidase activity [38–40]. In the presentstudy, we investigated the anti-inflammatory ⁄ antioxi-dant potential of these compounds by examining theirscavenging effects...
- 9
- 331
- 0
Báo cáo khoa học: Tryptophan 243 affects interprotein contacts, cofactor binding and stability in D-amino acid oxidase from Rhodotorula gracilis ppt
... Glu322), con-necting b-strands F5 and F6 and the negatively chargedresidues belonging to the a-helices I3¢ and I3¢¢ of the other monomer and (b) the interactions in the core of the dimer (where ... hydrophobic regions during the urea-induced unfolding of W243 mutants compared with the wild-type DAAO.Kinetics of reconstitution of apoprotein with FADBecause of the weaker binding of the FAD cofactor ... containing the peroxi-somal targeting signal) to the bab motif, known as the dinucleotide-binding domain [2], and on the other sideto the long loop following the b-strand I7 containingTrp243...
- 9
- 304
- 0
Báo cáo khoa học: "Smaller Alignment Models for Better Translations: Unsupervised Word Alignment with the 0" potx
... Jason Riesa forproviding the Arabic-English and Chinese-Englishhand-aligned data and the alignment visualizationtool, and Chris Dyer for the Czech-English hand-aligned data. This research ... translations (i.e., the size of the lexical weighting table) is reduced. The ˜φsing.column shows the average fertility of once-seen source words. ForCzech-English, the year refers to the WMT shared ... using MIRA (Chi-ang et al., 2008). We used two 5-gram languagemodels, one on the combined English sides of the NIST 2009 Arabic-English and Chinese-Englishconstrained tracks (385M words), and...
- 9
- 304
- 0
Báo cáo khoa học: G protein-coupled receptor-induced Akt activity in cellular proliferation and apoptosis pptx
... bothdirectly, through the interaction of Gbc subunits with PI3K, and indirectly, through the GPCR transactiva-tion of RTKs and integrins. We shall also examine the downstream signaling and physiological ... activation ⁄ inhibition of adenylyl and guanylylcyclases, and the opening and closing of ion channels.In this review, we shall investigate the ability of GPCRs to activate Akt signaling pathways bothdirectly, ... pathwaysmediating the GPCR control of cell cycle progressionare extensive [2], with all pathways converging in the nucleus to regulate the expression, localization, activity or stability of a small number of...
- 12
- 392
- 0
Tài liệu Báo cáo khoa học: Dual P2Y12 receptor signaling in thrombin-stimulated platelets – involvement of phosphoinositide 3-kinase b but not c isoform in Ca2+ mobilization and procoagulant activity pdf
... Protease-acti-vated receptors 1 and 4 do not stimulate Gi signalingpathways in the absence of secreted ADP and causehuman platelet aggregation independently of Gi signal-ing. Blood 99, 3629–3636.14 ... 3integrin activation and platelet aggregation [18]. We and others have shown that both the PI3-Kb and PI3-Kc isoforms contribute to the P2Y12-mediatedstabilization of platelet aggregates ... responsible for the prolonged Ca2+response and for the subsequent increases in procoagulant activity and coagulation. Takentogether, these results demonstrate a dual P2Y12-dependent signaling mech-anism,...
- 15
- 565
- 0
Từ khóa: báo cáo khoa học mẫubáo cáo khoa học y họcbáo cáo khoa học sinh họcbáo cáo khoa học nông nghiệpbáo cáo khoa học lâm nghiệpbáo cáo khoa học thủy sảnBáo cáo thực tập tại nhà thuốc tại Thành phố Hồ Chí Minh năm 2018Nghiên cứu sự biến đổi một số cytokin ở bệnh nhân xơ cứng bì hệ thốngBáo cáo quy trình mua hàng CT CP Công Nghệ NPVMột số giải pháp nâng cao chất lượng streaming thích ứng video trên nền giao thức HTTPGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitNGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWAN SLIDEPhối hợp giữa phòng văn hóa và thông tin với phòng giáo dục và đào tạo trong việc tuyên truyền, giáo dục, vận động xây dựng nông thôn mới huyện thanh thủy, tỉnh phú thọPhát triển du lịch bền vững trên cơ sở bảo vệ môi trường tự nhiên vịnh hạ longPhát hiện xâm nhập dựa trên thuật toán k meansNghiên cứu, xây dựng phần mềm smartscan và ứng dụng trong bảo vệ mạng máy tính chuyên dùngNghiên cứu tổng hợp các oxit hỗn hợp kích thƣớc nanomet ce 0 75 zr0 25o2 , ce 0 5 zr0 5o2 và khảo sát hoạt tính quang xúc tác của chúngSở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIXGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtBÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘIChiến lược marketing tại ngân hàng Agribank chi nhánh Sài Gòn từ 2013-2015HIỆU QUẢ CỦA MÔ HÌNH XỬ LÝ BÙN HOẠT TÍNH BẰNG KIỀMTÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲQUẢN LÝ VÀ TÁI CHẾ NHỰA Ở HOA KỲ