Báo cáo khoa học: Optimization of an Escherichia coli system for cell-free synthesis of selectively 15N-labelled proteins for rapid analysis by NMR spectroscopy pdf

Tài liệu Báo cáo khoa học: Catabolite repression in Escherichia coli – a comparison of modelling approaches docx

... phosphotransferase system in Escherichia coli is a transport and sen-sory system and, in this function, is one of the key players of cataboliterepression. Mathematical modelling of signal transduction ... range of different condi-tions, allowing for an extensive analysis of the strainsat hand.Dynamics and time hierarchiesTo show an application of their model, Nishio et al.[15] simulated an ... sophisticated methods for sensitivity analysis, parameter analysis and parameter estimation,50 parameters (34%) could be estimated.In particular, the analysis of mutant strains offersthe possibility...

Báo cáo khoa học: EspB from enterohaemorrhagic Escherichia coli is a natively partially folded protein pptx

... Nakagawa for access to light scattering, and Miyo Sakai for per-forming ultracentrifugation. This work was supported,in part, by grants-in-aid for scientific research from theJapan Ministry of Education, ... ultra-centrifugation, multiangle laser light scattering andheteronuclear NMR. The results of our analyses allowus to understand the conformational property of EspBand predict its role in bacterial ... ANS fluorescence, rather than reflect-ing additional conformational changes in EspB.Urea unfoldingUrea-induced unfolding transitions of EspB weremonitored by far-UV CD spectroscopy. Plots of...

Tài liệu Báo cáo khoa học: The resident endoplasmic reticulum protein, BAP31, associates with c-actin and myosin B heavy chain Analysis by capillary liquid chromatography microelectrospray tandem MS ppt

... identified in the first pass analysis were manually interpreted (Table 1). Of the initial 51 tandem mass specta obtained by LC-MS/MS analysis of the 190 kDa band 45 were selected for asecond pass identification ... masses of 33 kDa and 28 kDa,respectively, and two additional protein bands at apparentmasses of 42 and 190 kDa [9]. For sequence analysis, theBAP31 immunoprecipitation protocol was scaled up andthe ... documented. In many instances, a definite answermight require a detailed sequence analysis, typically by N-terminal sequencing (using Edman degradation) or by tandem MS. In the latter case, each analysed...

Báo cáo khoa học: Optimization of an Escherichia coli system for cell-free synthesis of selectively 15N-labelled proteins for rapid analysis by NMR spectroscopy pdf

... synthesis of 15N-labelled proteins (Eur. J. Biochem. 271) 4093 Optimization of an Escherichia coli system for cell-free synthesis of selectively 15N-labelled proteins for rapid analysis by NMR spectroscopy Kiyoshi ... preparation of 15N-labelled samples of hCypAand analysis by NMR spectroscopy, the performance of the cell-free expression system was explored with respect to anumber of parameters. We ﬁrst examined ... inimalusage of 15N-labelled amino acid, and examined for thepresence of metabolic by- products which could interferewith the NMR analysis. Yields of u p to 1.8 mg of humancyclophilin A per m L of reaction...

Báo cáo khoa học: Optimization of P1–P3 groups in symmetric and asymmetric HIV-1 protease inhibitors pptx

... and newcompounds with significantly different resistance profiles inparticular.AcknowledgementsWe thank Mrs Terese Bergfors for reading the manuscript andDr Mats Sandgren for preparation of ... the flaps form a roof over the activesite and the ligand. The flaps cover to a large extent thebound ligand. This arrangement is advantageous for thedesign of inhibitors, because it offers a ... purification and substrate analysis of bacteriallyexpressed HIV protease by means of monoclonal antibody.EMBO J. 7, 1785–1791.7. Debouck, C. (1992) The HIV-1 protease as a therapeutic target for AIDS....

Báo cáo khoa học: "Parsing with an Extended Domain of Locality" pot

... re- entrancy between the value of agr feature struc- tures at the anchor (verb) node and the subject node. Notice the re-entrancy between the anchor node and the substitution node for the ... instantiates the agr feature of the anchor node as 3sg which has the effect (due to the re- entrancy in the unanchored tree) of instantiating the agr feature at the subject node as 3sg. Anchored ... the an- chor of the tree and the nodes it imposes con- straints on. If all dependencies relevant to the parser can be captured in this way then, once an elementary tree has been anchored by...

Báo cáo khoa học: Optimization of D-amino acid oxidase for low substrate concentrations – towards a cancer enzyme therapy pdf

... correspondingly enhanced production of H2O2.A B C D Fig. 5. Activity and cytotoxicity of wt-DAAO and mutants. (A) Com-parison of the activity of wt-DAAO and mutants with 0.1 mMD-Alaand at 2.5% ... estimation of thespectra of intermediates, of rate constants, and of the con-centration of intermediates as a function of time. The sameprogram was used to simulate kinetic processes [35]. Of relevance ... surgery and radiother-apy, is widely used for the treatment of malignant dis-ease. Unfortunately, and as is widely known, theselectivity of most drugs for malignant cells remainsinsufﬁcient. An...

Báo cáo khoa học: ˚ cDNA cloning and 1.75 A crystal structure determination of PPL2, an endochitinase and N-acetylglucosaminebinding hemagglutinin from Parkia platycephala seeds potx

... A for 5 min, followed by gradients of 0–30% of solution B(66.6% acetonitrile in A) for 5 min, 30–40% B for 30 min,40–70% B for 5 min, 70–80% B for 10 min, 80–100% B for 5 min, and 100% B for ... seeds of Canavalia ensiformis. J Mol Biol 254,237–246.42 Van Scheltinga ACT, Hennig M & Dijkstra BW (1996)The 1.8 A˚resolution structure of hevamine, a plantchitinase ⁄ lysozyme, and analysis ... Mol Plant-Microbe Interact 7, 48–57.44 Copley RR & Barton GJ (1994) A structural analysis of phosphate and sulphate binding sites in proteins. Estimation of propensities for binding and conserva-tion...

Báo cáo khoa học: "Redundancy Ratio: An Invariant Property of the Consonant Inventories of the World’s Languages" pdf

... span amuch larger range for the vowel inventories than for the consonant inventories.The significance of the difference in the nature of the distribution of RRs for the vowel and the conso-nant ... inventories of theworld’s languages. Through rigorous statis-tical analysis, we find that this redundancyis an invariant property of the consonant in-ventories. The statistical analysis further ... redundancy is found in every level of granularity of a system. For instance, in biologi-cal systems we find redundancy in the codons (Lesk,2002), in the genes (Woollard, 2005) and as well inthe proteins...

Báo cáo khoa học: LIN54 is an essential core subunit of the DREAM / LINC complex that binds to the cdc2 promoter in a sequence-speciﬁc manner ppt

... efﬁciency.AcknowledgementsWe thank all members of the laboratory for their sug-gestions and critical reading of the manuscript. Thiswork was supported by grants from the DFG (575 ⁄ 4-1and TR17-B1) to SG.References1 ... DHCH and D Nmutants in a panel of different cell lines. Although difﬁ-cult to overexpress, distinct bands corresponding toLIN54 and the DHCH and DN mutants could bedetected (marked by asterisks). ... N, Lucibello FC, Engeland K & Muller R (1998) Anew model of cell cycle-regulated transcription: repres-sion of the cyclin A promoter by CDF-1 and anti-repression by E2F. Oncogene 16, 2957–2963.34...

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