... transcarbamoylation reaction catalyzed
by HypF
The formation of the HypE- thiocyanate involves first the
carbamoylation of the C -terminal c ysteine of HypE b y
interaction with HypF, then the release of HypF and ... future whether they can transfer the
carboxamido moiety to the HypC · HypD complex.
Analysis of the transcarbamoylation reaction cat...
... mea-
sure the mobility of the components in the PGCs. The
medaka embryo was peeled off the chorion, and the
segment containing the part of PGCs was excised for
observation by microscopy and FCS ... constants of 11.7, 12.9 and
5.4 lm
2
Æs
)1
, respectively, in the part of the cytoplasm
outside of the granules. FRAP analysis in the gran-
ules provided t...
... [32].
The Lsm1 and Lsm8 polypeptides are most similar in
the regions of the Sm1 and Sm2 motifs. These motifs
form the Sm-fold, the hallmark of the Sm-like pro-
teins, consisting of a five-stranded ... investigate the requirement for different domains of
the Lsm1 and Lsm8 proteins in their function and
localization, we created a series of mutant and hybrid...
... enzymes.
The most significant difference between the amphi-
oxus and the other retinal reductases was, nevertheless,
their preference for the NADH cofactor. To our
knowledge, these are the first ... out for 15 min at 37 °Cin
0.5 mL. The amount of protein used in the reaction mixture
was 15 lg. The reaction was terminated by the addition of
an equal volume of cold...
... 2A). The binding
of raptor to these two variants was similar (Fig. 1D),
implying that the loss of raptor binding on removal of
the first 17 residues may be accounted for simply by
the loss of the ... define the
RAIP motif [25]. The use of the rat protein also has
the advantage that there is no cross-reactivity of the
(P)Ser64 antibody with other sites, which is...
... structure
found in the large rRNA of prokaryotic and eukaryotic
ribosomes [1,2]. At present, they are subdivided on the basis
of the structure of the genes and the corresponding proteins
into holo-RIPs and ... K. (1987) The
mechanism of action of ricin and related toxic lectins on eu-
karyotic ribosomes. The site and the characteristics of the mod-
ifi...
... [24], and the C1s
protease of the C1 complex of complement [25]. Thus,
the Trp band can be regarded as a characteristic
fingerprint of the native fold of PSP-I and PSP-II.
The near-UV CD spectra of ... induced by Zn
2+
was reversed by the addi-
tion of EDTA to the sample (Fig. 2A). On the other
hand, no significant decrease in the heterodimer stabil-
ity wa...
... min; then 10 lLofincreasing
concentrations of PCI or ProNtPCI were added and
the measures were continued for 2 min. The slope of the
first part of the assay corresponded to m
o
and the slope
of the ... in vitro and in vivo [7]. There are not
many examples of the role of the pro-regions in small
disulfide-rich proteins. In these proteins, the folding process...
... Band 4 showed more RNase E
and PNPase than RhlB and enolase. By contrast,
Bands 1, 2, 3 and 5 showed roughly equivalent
amounts of each of these proteins in the four samples.
Interestingly, Band ... in
each band, indicating that the bands excised from the BN-PAGE gel are composed of different proteins. (C) Western blot of the second
dimension SDS ⁄ PAGE separation...
... mutant toxins and the
analysis of their binding activity to the receptor to
investigate the role of mutated amino acid residues; (b)
the analysis of the influence of the modification of the
cysteine ... osmotic swelling and bursting of
the cell, leading to disruption of the midgut tissue and
death of the insect [16].
The three-dimensional structur...