... Vector System ( [E 1- ,E2b-]AD) [3] was used for construction of human BMP second-generation adenoviral vectors ( [E 1-, E2b-]ADhBMP6, [E 1-, E2b-]ADGFP&hBMP4, and [E 1-, E2b-]ADGFP&hBMP6), ... [E 1-, E2b-]ADhBMP6, and [E 1-, E2b-]ADGFP&hBMP6, were validated by PCR, RT-PCR, and Southern blot analysis. The titers of the purified [E 1-, E2b-]ADGFP&hBMP4, [E 1-, E2b-]ADhBMP6, and [E 1-, E2b-]ADGFP&hBMP6 ... activities of recombinant human BMP6 in E 1- (ADhBMP6) and [E 1-, E2b-] ( [E 1-, E2b-]ADGFP&hBMP6, and [E 1-, E2b-]ADhBMP6) adenoviral vectors were compared in both in vitro and in vivo models. Similar...