Báo cáo khoa học: Protection of chylomicron remnants from oxidation by incorporation of probucol into the particles enhances their uptake by human macrophages and increases lipid accumulation in the cells ppt

... cell induction by chylomicron remnants (Eur. J. Biochem. 271) 2427 Protection of chylomicron remnants from oxidation by incorporation of probucol into the particles enhances their uptake by human macrophages ... the protection of chylomicron remnants from oxidation enhances, rather than inhibits, their induction of lipid accumulation in macrophages. The enhancement of lipid accumulation in macrophages by ... the induction of lipid accumulation in the cells by the particles is enhanced whenthey are protected from oxidation, we investigated the mechanism of this effect by comparing the in uence of CRLPs...

Tài liệu Báo cáo khoa học: TEC family kinases in health and disease – loss-of-function of BTK and ITK and the gain-of-function fusions ITK–SYK and BTK–SYK pptx

... disease, thereby providing the missing data. The phenotype of XLA and the potential of BTK and ITK inhibitors The outcome of defective BTK signaling in humanshas been described previously in detail ... directly involved in ligandbinding, presumably abolishing the interaction withsignaling partners. The remaining mutations alteramino acids located outside the ligand-binding pocket and reduce ... the linker B kinase region of SYK. The PH domain of TFKs usually binds to phosphatidylino-sitol-3,4,5-trisphosphate, thereby bringing them in close proximity to other membrane-tethered signalingproteins....

Tài liệu Báo cáo khoa học: Toggle switches, pulses and oscillations are intrinsic properties of the Src activation/deactivation cycle doc

... conformation, the SH2 domain binds to pYion the C-terminal tail, and the Src homology 3 (SH3) domain binds to the linkerbetween the SH2 and kinase domains at the back of the small lobe, preventing the ... in (B) show the time point of step changes in [RPTP]. The corresponding [RPTP] values, 117.5, 126.5 and 135.5 nM, are indi-cated by dashed lines 1–3 in (A) and shown by upper line in (B). The ... structure of the Abl/Arg and Tec kinase families (in particular, of the c-Ablkinase) resembles the SFK structures. The c-Abl kinasepossesses three domains (SH2, SH3 and the two-lobekinase domain),...

Tài liệu Báo cáo khoa học: Competition between innate multidrug resistance and intracellular binding of rhodamine dyes pdf

... mechanism of rhodamine dyes,agents allowing the enhanced uptake of rhodaminedyes inhibited the efﬂux of these dyes from the cells. The efﬂux of rhodamine 123 and TMRM was inhib-ited by NBD-Cl and ... including the dye adsorbed to the sur-face of the cells. To avoid complication of the uptake studies by active pumping of the dyes into the mitochondria, dye uptake was measured routinely in the presence ... are excluded from these cells by MRP1. On the other hand, the uptake of rhodamines into cells respiring with their coupled mitochondria demonstrated diminished sensi-tivity to NBD-Cl and MK571....

Tài liệu Báo cáo khoa học: A facile method for expression and puriﬁcation of the Alzheimer’s disease-associated amyloid b-peptide pdf

... : 2000 in blocking solution for 30 min. Cells wererinsed in NaCl ⁄ Piseveral times and incubated in blockingserum containing anti-mouse IgG (Vectastain) for a fur-ther 30 min. Staining was ... morerapid handling using the devices and loss of peptide on the column resin. The only detriment of the peptidesproduced here is the fact that they contain an exo-genous N-terminal methionine. However, ... capacity by produc-ing and cloning genes for familial mutants in the 19–23 region of Ab(L1–40) and Ab(L1–42), and expressing and purifying the peptides. The procedure is of course not limited to the...

Tài liệu Báo cáo khoa học: Verprolin function in endocytosis and actin organization Roles of the Las17p (yeast WASP)-binding domain and a novel C-terminal actin-binding domain doc

... domain structure. Schematic of Vrp1p showing the Vrp1p truncations and mutant proteins used in this study and their various known domains: actin-bindingdomains, Hof one trap (HOT) domain, and LBD. ... novel actin-binding VH2-Cdomain in C-Vrp1p364)817may explain why mutations in the N-terminal actin-binding WH2 domain (WH2-1)or even deletion of the entire N-terminal module of Vrp1p did ... 2007 The Authors Journal compilation ª 2007 FEBS 4121Verprolin function in endocytosis and actin organizationRoles of the Las17p (yeast WASP)-binding domain and a novelC-terminal actin-binding...

Tài liệu Báo cáo khoa học: Multiple promoters regulate tissue-speciﬁc alternative splicing of the human kallikrein gene, KLK11⁄hippostasin ppt

... derived from the human KLK11 geneWe determined the nucleotide sequences of 15 clones of the PCR products from the human prostate by oligo cap RACE in order to determine the transcrip-tion initiation ... the regulation and characteristics of these isoforms are unknown. We identified the first three exons (1a, 1b, and 1c) by determining their transcription initiation sites. Exon 1b con-tained the ... expressing both isoforms of KLK11, and used human a neuroblastoma cell line,KP-N-YN, because of the transfection efficiency and expression of both KLK11 isoform 1 and isoform 2 in this cell line....

Tài liệu Báo cáo khóa học: Active-site residues and amino acid speciﬁcity of the bacterial 4¢-phosphopantothenoylcysteine synthetase CoaB pptx

... from D-pantothenate. The Dfp ﬂavoprotein catalyzes the synthe-sis of the coenzyme A precursor 4¢-phosphopantetheine from 4¢-phosphopantothenate and cysteine using the cofac-tors CTP and ﬂavine mononucleotide ... conserved in CoaB proteins. Residues of the N-terminal part of the Escherichia coliCoaB domain that are conserved in CoaB (Dfp) proteins from all kingdoms of life and that were examined in the present ... important for the second-half reaction (and that isprobably involved in cysteine binding), the in vitro activity of further mutants was measured in the presence and absence of cysteine. It turned...

Tài liệu Báo cáo khoa học: Molecular characterization, phylogenetic relationships, and developmental expression patterns of prion genes in zebraﬁsh (Danio rerio) doc

... because in terms of func-tion, the fish intestine is highly regionalized both in the larva and in the adult [38]. The posterior segment of the fish intestine is the absorption site of intact pro-teins, ... and in epithelial cells lining the digestive tract lumen[43,44]. Considering the importance of the intestinalbarrier in the process of oral prion infection, this find-ing might help clarify the entry ... FEBSdepends in part on the homology between the donorpathogen protein and the natural physiological proteinpresent in the receiver, both in the amino acid sequence of the protein and in its tridimensional...

Tài liệu Báo cáo khoa học: Endogenous expression and protein kinase A-dependent phosphorylation of the guanine nucleotide exchange factor Ras-GRF1 in human embryonic kidney 293 cells pptx

... (F340) increases, whereas the ﬂuorescence intensity at 380 nm (F380) decreases upon the binding of Ca2+. The change in the ratio of F340 ⁄ F380 determined the change in Ca2+concentrationinside ... Ca2+⁄CaM-binding IQ domain that is involved in the activa-tion of Ras-GRF1.Stimulation of all the splice variants of the Gs-coupledserotonin receptor 5-hydroxytryptamine7(5-HT7) increases intracellular ... Forskolin increases aden-ylyl cyclase activity and induces the phosphorylation of Ser916 in the mouse Ras-GRF1 sequence [12] and of Ser898 in the rat sequence [14]. To test whetherserotonin stimulated...

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