Báo cáo khóa học: Membrane transport of fatty acylcarnitine and free L-carnitine by rat liver microsomes pot

... Membrane transport of fatty acylcarnitine and free L-carnitine by rat liver microsomes Jason M. Gooding, Majid Shayeghi and E. David SaggersonDepartment of Biochemistry and Molecular ... trans-ER membrane transport of palmitoylcarni-tine together with an ER transport process for CoASH,the cosubstrate for microsomal CAT [12]. In support of the notion of fatty acylcarnitine transport ... palmitoyl-CoA, albeit athigher concentrations in the absence of BSA, permeabilizes rat liver microsomes [31]. AEAT is inhibited by highconcentrations of its fatty acyl-CoA substrate [25].Figure 3B shows...

Báo cáo khoa học: Catalytic digestion of human tumor necrosis factor-a by antibody heavy chain pot

... N-terminalsequencing.) At 8 h of incubation (lane 2), the bandsat 19 and 20 kDa had disappeared, and two clearbands appeared at 15.0 and 13.3 kDa. These bandsare thought to be fragments of hTNF-a.At20hofincubation ... After 8 h of incu-bation, the bands at 19 and 29 kDa had disappeared, and two clearbands appeared at 15.0 and 13.3 kDa. These bands are thought tobe fragments of hTNF-a. After 20 h of incubation, ... conformation of ETNF-6mAbSequencing of the cDNA of the variable region of ETNF-6 mAb was performed, and this was followed by molecular modeling of its three-dimensional struc-ture. The heavy chain of...

Báo cáo khoa học: "Generalized Encoding of Description Spaces and its Application to Typed Feature Structures" potx

... must be that F , so by statictypability, the lemma holds.Intro F and Intro F : and F , so and F are con-sistent. By bounded completeness, F and F . By upward closure,F F and by static typability,F ... unifying the representatives of and , one ( ) for unifying the representatives of and , and one ( ) for unifying the representatives of and . Notice that the set of representatives formust be ... TFS of type bool, and this value is notshared with any other structure in the TFS. If thevalues of MOD and PRD are both bool-typed, then if5The sole exception is a TFS of type, which by deﬁnitionbelongs...

Tài liệu Báo cáo khoa học: Membrane orientation of laminin binding protein An extracellular matrix bridging molecule of Leishmania donovani pdf

... understanding and written consent of thesubject) [7] and subsequently maintained in BALB/c mice by intravenous passage every 6 weeks. Promastigotes, obtained by in vitro transformation of liver and ... was terminated by chilling on ice and adding 4 lLof0.25MPMSF and 60 lLof0.5%(w/v)egg white trypsin inhibitor in 0.15MNaCl. From theprotease treated and untreated membrane preparations,LBP ... identiﬁed by TID involves theintramembraneous region and the 27 kDa, unable toincorporate TID, involves the laminin binding region.Fig. 4. Phase separation of LBP by Triton X-114 and external...

Báo cáo khoa học: Membrane anchoring of diacylglycerol lactones substituted with rigid hydrophobic acyl domains correlates with biological activities ppt

... constrained analogues of diacylglycerol (DAG). 27.Modulation of membrane translocation of proteinkinase C (PKC) isozymes a and d by diacylglycerol Membrane interactions of diacylglycerol derivatives ... initially tothe plasma membrane and only later to the nuclear membrane and other internal membranes (Fig. 1C and D, top rows). Additionally, the PKC-a translocationinduced by 3 and 4 appears to be ... modulators of PKC both in vitro and in intact cells; however, differences in the patterns of PKC translocation to membranes were apparentbetween DAG lactones 1 and 2 on one hand, and DAG lactones 3 and...

Báo cáo khoa học: Membrane binding of SRP pathway components in the halophilic archaea Haloferax volcanii docx

... presently of the nature of the FtsY membrane interaction in Archaea. Moreover, the steps leading todeliveryofSRPtothearchaealmembranehaveyettobedescribed.Towards an understanding of the membrane ... soluble and a membrane- associated protein [11]. While the precise roles and temporal positions of SRP and FtsY in the bacterialSRP cycle remain topics of on-going investigation [9], membrane ... comprised of 7S RNA, SRP19 and SRP54 subunits,is more reminiscent of its eukaryal counterpart, yet alsopossesses Archaea-speciﬁc traits in terms of the makeup of its subunits and mode of assembly...

Báo cáo khoa học: Membrane distribution of epidermal growth factor receptors in cells expressing different gangliosides doc

... glycolipid composition of the plasma membrane by altering the gangliosidebiosynthetic activity of the cell, while maintaining thenormal process of intracellular transport and membrane insertion ... changes of membrane distribution associated with its activationstatus.EGFr and GD3 localization in CHO-K1 cell membranesHaving demonstrated a converse segregation of GD3 and EGFr, by biochemical ... plasma membranes and intracellular membranes(Fig. 5). A comparison between GD3 and EGFr membrane distribution revealed a minor separation (Fig. 5A–D). Tobetter deﬁne the localization of GD3 and...

Tài liệu Báo cáo khoa học: Autolytic activity of human calpain 7 is enhanced by ESCRT-III-related protein IST1 through MIT–MIM interaction pptx

... PAGE (15% gel) and analyzed by WB with mAb against GFPto detect proteolysed fragments of mGFP–calpain 7–Strep. Bands of full-length WT and C290S are indicated by the arrow, and those of 30 K are ... bands were detected by the chemiluminescencemethod. Membranes were also stained with CBB. Bands of GST–MIT–pHis and GST–pHis in the pulldown products are indicated by arrows.Enhancement of ... isgenerated by proteolytic activity of mGFP–cal-pain 7–Strep itself, not by potentially contaminatingproteases in the preparations, and that MIT–MIMinteraction enhances the autolytic activity of...

Tài liệu Báo cáo khoa học: Mitochondrial targeting of intact CYP2B1 and CYP2E1 and N-terminal truncated CYP1A1 proteins in Saccharomyces cerevisiae ) role of protein kinase A in the mitochondrial targeting of CYP2E1 pdf

... NADPH, and 4 mm NDMA in a 1.0 mL ﬁnalvolume. The reaction mixture was incubated at 37 °C for30 min, and the reaction was stopped by the addition of 0.1 mL of 25% zinc sulfate and 0.1 mL of saturated ... concentrations of digitonin.It is known that low concentrations of digitonin (about0.05%) selectively damage the outer membrane, and higher concentrations (about 0.1%) damage the inner membrane. ... yeaststrain, and Dr Andy Dancis for providing with FDX- and FDXR-depleted strains. We also thank members of the Avadhani laboratory for valuable suggestions and comments. This research was supported by...

Tài liệu Báo cáo khoa học: Differential effects of Mxi1-SRa and Mxi1-SRb in Myc antagonism ppt

... including proliferation, apopto-sis, differentiation, and so on. Isoforms of numerousproteins have been studied and compared in this man-ner, including alternative isoforms of members of thep53 ... Expression constructs weregenerated encoding Myc-tagged versions of these twoisoforms, as well as of the Mxi1-WR isoform that lacksthe SID, and shown to give rise to proteins of theexpected size expressed ... stability, and Myc bio-logical activity. Normal regulation of Myc activityoccurs by mechanisms that inﬂuence the Myc proteinper se [7], and also through the functions of relatedmembers of the...

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