Báo cáo khóa học: Endoplasmic reticulum-associated degradation of glycoproteins bearing Man5GlcNAc2 and Man9GlcNAc2 species in the MI8-5 CHO cell line docx
... Endoplasmic reticulum-associated degradation of glycoproteins
bearing Man5GlcNAc2 and Man9GlcNAc2 species in the MI8-5
CHO cell line
Franc¸ois Foulquier, Sandrine Duvet, Andre
´
Klein, Anne-Marie ... converted into Man4Glc-
NAc2, in contrast with the small amount of Man6GlcNAc2
originating from the Man9GlcNAc2 species.
ERAD of glycoproteins bea...
... activity of the reaction
intermediate, FVa
int
. This facilitated the determination of
loss of FVa cofactor activity during the initial stage of
inactivation and minimized the in uence of the k
306
Table ... Arg506.
Fig. 4. Kinetic analysis of the inactivation of FVa by APC in the pres-
ence and absence of heparin. Initial rates of FVa inactivation were...
... petB, and to domain IV
of the petB intron. The binding of the homodimer of
HCF152 in this region somehow stabilizes the splicing
products, possibly by folding the RNA into the correct
splicing ... located at the C-terminus of the
protein although binding of the C-terminus half (CH) is
only half of the full-length protein (Fig. 3). Interestingly, the
bindi...
... changes of the band pattern were
observed. The bands of partially dissociated urease
(a
m
b
n
) became faint as well as the band of the
b-subunit monomer. In contrast, the intensity of the
band (1; ... (lane 3), the band of the b-subunit became very faint. Some new bands between bands 1 and 2 became clearer and several
bands around bands 4 and 5 also became d...
... in the absence of calpain (Fig. 5). Appar-
ently, calpain affects the strength of insulin signaling
to AKT, and p70-S6 kinase. The detailed mechanism
and the further biological consequences of ... role of calpain
in insulin signaling. Target phosphorylation of insulin
was reduced in calpain-negative cells (capn4 knockout
MEFs) and restored upon lentiviral introdu...
... protons of GlcNAc, and the H3-equitorial
and H3-axial protons of N-glycolylneuraminic acid
[23–26]. The
1
H-NMR spectrum of cRBCs within the
region of interest is shown in Fig. 3 and the list of
important ... treatment of the
collected pools to determine the distribution of sialic
acid. Next, we completed online MS ⁄ MS analysis of
the major species; som...
... particular choice in
the number of seed attributes, and in the source of
instances passed as input to the system:
• number of input seed attributes: seed attributes
are provided either for each of the ... class label, and consists of four stages:
1) identification of a noisy pool of candidate at-
tributes, as remainders of queries that also contain
one of the...
...
REFERENCING AND FOCUSSING
For referencing and other phenomena like
focussing, the simple approach of only
allowing a schema instance as a filler is not
sufficient. We therefore included in our ... feasibility of the approach of
using a semantic representation as an interlingua
in a practical application will be investigated and
demonstrated by translating titles of...
... was induced by binding
of elongin B. The conformational change of elongin C was investigated by
measuring changes in the intramolecular FRET signal of elongin C linked to
cerulean and citrine ... FLIM analysis of interaction between elongin B and elon-
gin C in CHO- K1 cells. (A) Cellular localization of elongin B linked to
cerulean and elongin C linked to citrine. Ch...
... N-
terminal motifs are involved in ribosome binding, and three
of the C-terminal domains are required for mRNA
recognition [6,75,76], with the last domain involved in the
autoregulation of S1 ... PSRP-2 contains two
RNA-binding domains which occur in mRNA processing/
stabilizing proteins (e.g. U1A snRNP, poly(A)-binding
proteins), suggesting a possible role for it in the...