Báo cáo khóa học: Assembly of the silk fibroin elementary unit in endoplasmic reticulum and a role of L-chain for protection of a1,2-mannose residues in N-linked oligosaccharide chains of fibrohexamerin/P25 ppt
... 2003
Assembly of the silk fibroin elementary unit in endoplasmic reticulum
and a role of L-chain for protection of a1 ,2-mannose residues
in N-linked oligosaccharide chains of fibrohexamerin/P25
Satoshi ... the assembly of the
elementary unit of fibroin in ER
It was demonstrated in this study that the elementary
un...
... human
joints affected by RA. Staining of sPLA
2
-IIA in an inactive RA tissue
was rare, and only a few synovial lining regions (dark arrowheads)
showed weak immunoreactivity (A) . In an active RA ... Naiman BM,
Wachtmann TS, Umland JP, Pandher K, Lapointe JM,
Saha S, Roach ML et al. (2003) Impaired in ammatory
and pain responses in mice lacking an inducible prosta-
glandin E sy...
... receptor-
associated death domain and of the TNF-related
apoptosis-inducing ligand, leading to the activation of
death-receptor signaling and caspase-mediated apopto-
sis [59]. Activated Akt phosphorylates ... complex that includes b-arrestin, protein
phosphatase 2A and Akt. Protein phosphatase 2A
inactivates Akt, thereby relieving Akt’s inhibition of
glycogen synthase kinas...
... were of
analytical grade.
Yeast strains and growth media
The genotypes and sources of the S. cerevisiae strains are
described in Table 2. The ISP deletion strain was
prepared in accordance with the ... just after the addition of ISP and Qcr10p).
On the other hand, in the transition from the 500 kDa
band to the 670 kDa band, a structural rearrangement
of...
... rearrangements include an inversion
of cagQ, a duplication of cagA and cagB associated with cagA degeneration, and a more complex rearrangement comprising all cag genes
except cagA and a second inversion of ... variations of the general gene arrange-
ment. For example, some strains isolated from Ameri-
can Indians have a duplication of cagA (in a
nonfunctional f...
... research on the interaction between WAT
domains of AChE catalytic subunits and the PRAD
domains of ColQ and PRiMA [38,39,45,46], the fact
that G
4
AChE interacts with a noncatalytic subunit in
nematodes ... californica AChE is 986 A
˚
3
.
Molecular modeling of monomeric C. intestinalis
AChE catalytic subunits with the PRAD domain
of ColQ indicates that the WAT doma...
... with the latter being dominant.
The fluorescence and near-UV CD spectra point to tight packing of the
aromatic residues in the core of the protein. From the near-UV CD signals
and activity data as ... present in all members
of the PLD superfamily. However, there is low similar-
ity in the remaining parts of the molecules. Most mam-
malian PLDs contain a...
... Kaneko
1
, Yuanying Ding
1
, Kazuhiro Ogawa
2,
*,
Miki Yoshizawa
1
, Masaki Kawamura
1
, Kazuhisa Takeda
1
, Tadashi Yoshida
3
and Shigeki Shibahara
1
1 Department of Molecular Biology and Applied ... HepG2 and HeLa cells
were cultured for 48 h under normoxia or hypoxia, and then harves-
ted. The microsome fraction was prepared and used for the assay
of HO activity. The...
... N-terminal and b-he lical
domains participate in binding of the polysaccharide,
pullulan.
Comparison of the active sites of the model of IPU
and Dex4 9A
The active site structures of t he model of ... GGTGCT
gAGCTCAAGTGTGACTTtcGTCTAC
SacI
W402F CCGGTG
GTcGAcTTTGGTTtcACGCCC
SalI
E273Q ACGTACTGCTgTCCGGAA
AGtACtCCATGGCCGC
ScaI
D353N TCCAATCCGTtAGTC
TGgCCaTAGAACGCGC
Msc...
... 5¢-CTGGGAGGAGA
CAACAGCCTGGCAATC-3¢ for His120Asn, 5¢-TGGGTT
GATGCCAATGCTGACATCAAC-3¢ for His145Asn and
5¢-TGACATCGACACACCCC-3¢ for Asn149Asp.
Fluorescence spectra and thermal inactivation
studies
Fluorescence ... agmatinase activity of the Asn149Asp vari-
ant, it is clear that the interactions of arginase II with
l-arginine and agmatine are greatly altered by replace-
ment...