Báo cáo khoa học: DNA mediated disassembly of hRad51 and hRad52 proteins and recruitment of hRad51 to ssDNA by hRad52 pot
Báo cáo khoa học: DNA mediated disassembly of hRad51 and hRad52 proteins and recruitment of hRad51 to ssDNA by hRad52 pot
... DNA mediated disassembly of hRad51 and hRad52 proteins and recruitment of hRad51 to ssDNA by hRad52 Vasundhara M. Navadgi, Ashish Shukla, Rahul Kumar Vempati and Basuthkar J. RaoDepartment of ... aggregation states of hRad51 and hRad52 in the presence of 121-mer ssDNA and hRad52 mediated assembly of the ssDNA hRad51 complex. The changes in the proteinaggregation states were monitored by three ... 203presence of hRad52 renders much better binding of hRad51 to ssDNA even at lower concentrations of thelatter, thereby implying that hRad52 plays a role inthe recruitment of hRad51 to ssDNA. The...
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Tài liệu Báo cáo khoa học: DNA methylation-mediated nucleosome dynamics and oncogenic Ras signaling pptx
... signaling and loss of apoptosis [141].Silencing of FASL and TRAIL-R1, TRAIL-R2 and Caspase-8 expression by DNA methylation has beenlinked to resistance of small cell lung cancer cells to FASL and ... dedicated (by SKP) to Dr MK Pal,retired professor of biochemistry, University of Kaly-ani and Dr D Chattopadhyay, professor of biochemis-try, molecular biology and biotechnology, University of Calcutta, ... signaling and theresponse to chemotherapeutic agents. Activation of Ras in lipid rafts triggers DNA methylation and thesilencing of repair and apoptotic genes. The FAS geneis repressed by DNA methylation...
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Tài liệu Báo cáo khoa học: DNA adenine methylation changes dramatically during establishment of symbiosis pdf
... and HinfI for 3 h and recovered by ethanol precipitation. The precipitated DNA was dissolvedin 3 lL of water and added into the ligation mixture[100 ng each of landmark- and HinfI-adapter and ... containing 5 lLof10· PCR buffer (Takara, Tokyo, Japan), 4 lL of 2.5 mmeach dNTP mixture, 10 pmol each of CasA- and CasB-specific primers, 1 lL of ligation solution, and 1.25 U of Taq DNA polymerase ... whether the formation of clusteredspots was due to insufficient cleavage activity of HinfI,we obtained real and virtual RLGS images of Pseudo-monas syringae pv. tomato DC3000 DNA, which isclassified...
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Tài liệu Báo cáo khoa học: DNA modification with cisplatin affects sequence-specific DNA binding of p53 and p73 proteins in a target site-dependent manner pptx
... preparation of the p53 proteins, and Professor G.Melino for donation of plasmids pcDNA3-HA-p73b and pcDNA3-HA-p73d. This work was supported by IGA MH CR grant No. NC ⁄ 7574-3 and partly by GACR ... & Brabec V (2006) Recognition of DNA modified by trans-[PtCl2NH3(4-hydroxymethylpyridine)] by tumor suppressor protein p53 and character of DNA adducts of this cytotoxic complex. FEBS J 273, 301–314.38 ... comprises about 50% of 1,2-GG IACs, 25% of 1,2-AG IACs, 10% of 1,3-GNG IACs and interstrand crosslinks, and another2–3% of monofunctional adducts. It has been foundthat cisplatin cytotoxicity is related...
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Tài liệu Báo cáo khoa học: DNA strand exchange activity of rice recombinase OsDmc1 monitored by fluorescence resonance energy transfer and the role of ATP hydrolysis pptx
... renaturation of single-strand DNA (ssDNA) and assimilation of ssDNA into homologous super-coiled duplex DNA. J BiolChem 276, 41906–41912.10 Nara T, Hamada F, Namekawa S & Sakaguchi K (2001)Strand ... single and double stranded DNA binding activity. Binding to single stranded DNA stimulated a significant level of DNA dependent ATPase activity. OsDmc1 alsoshowed renaturation of complementary strands ... related to some criticalsteps in the conversion of inactive forms of protein to that of active forms. It is interesting and intriguing to note that in the absence of any nucleotide cofactor,OsDmc1...
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Báo cáo khoa học: Calmodulin-mediated regulation of the epidermal growth factor receptor doc
... additional and innovativeattention to clarify the mechanisms of action of thismodulator when operating at distinct levels withrespect to controlling the functionality and fate of thisreceptor. ... factor on binding of glycolytic enzymes to muscle cytoskeleton and theantagonistic action of calmodulin inhibitors. BiochemMed Metab Biol 50, 24–34.107 Llado´A, Tebar F, Calvo M, Moreto´J, ... yet fully understood.Complex oscillatory changes in the cytosolic concen-tration of Ca2+in response to different concentrations of EGF, and hence the number of occupied receptors,have been...
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Báo cáo khoa học: DNA-binding characteristics of the regulator SenR in response to phosphorylation by the sensor histidine autokinase SenS from Streptomyces reticuli doc
... amplified and then cleaved withEcoRI. Restricted fragments A and B (to delete site I), C and D (to delete site II), E and F (to delete site III), G and H (to delete the perfect inverted repeat) and ... characterization of the DNA- binding properties in response to phosphoryla-tion by the sensorkinase SenS.ResultsCloning of wild-type and mutant senSc and senRgenes and purification of fusion proteins As ... specifically to up-furS1 and up-hbpS1, and the phosphorylation of SenR by SenSP substantiallyalters its DNA- binding characteristics.Further bandshift assays using different amounts of purified SenR proteins...
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Báo cáo khoa học: DNA polymerase e associates with the elongating form of RNA polymerase II and nascent transcripts pot
... Academy of Finland to JES and HP and a grant by the German-Isareli Foundation for Scientific Research and Develop-ment to GK. We are grateful to Tomi Ma¨kela¨forgenerous gifts of antibodies and ... presence of radioactive UTP or dATP and the photoreactive DNA precursor BrdUTP, and subjected to UVcrosslinking. The photolabelled derivatives of Pols a, d and e werethen resolved by SDS ⁄ ... RD (2000)Nucleotide excision repair of DNA with recombinanthuman proteins: definition of the minimal set of factors,active forms of TFIIH, and modulation by CAK. GenesDev 14, 349–359.27 Navas...
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Báo cáo khoa học: Calpain-mediated degradation of reversibly oxidized protein-tyrosine phosphatase 1B docx
... constitution of PTP1B fragmentsobtained by calpain -mediated cleavage in the absence of oxidation.Fig. S2. Detection of PTP1B in wild-type and calpain-deficient cells by immunocytochemistry.Fig. ... degradation of PTP1B by cal-pain in vitro in order to prove that calpain is responsi-ble for the cleavage reaction, to identify the site of cleavage and the mechanism of the reaction. Oxidation of PTP1B ... 3.2Ô software (Thermo Electron Corp.) and the NCBI human protein database were used.AcknowledgementsThis work was supported by grants DFG He 551⁄ 11 (to PH) and DFG Bo 1043 ⁄ 6-2 (to FDB) and...
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Báo cáo khoa học: Thrombin-mediated impairment of fibroblast growth factor-2 activity doc
... followed by chemiluminescence (ECL;Amersham) and autoradiography.Bands were quantified using a calibrated imaging densi-tometer (GS 710; Bio-Rad Laboratories) and analyzedusing quantity one software ... levels of cytokines and cytokine receptors in patients with non-small cell lungcancer, and correlations with clinicopathologicalfeatures and prognosis. M-CSF-an independentprognostic factor. ... School of Medicine, Rome, Italy3 Department of Histology and Medical Embriology, University of Rome ‘Sapienza’, ItalyFibroblast growth factor (FGF)-2 belongs to the23-member family of FGFs...
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Từ khóa: báo cáo khoa học mẫubáo cáo khoa học y họcbáo cáo khoa học sinh họcbáo cáo khoa học nông nghiệpbáo cáo khoa học lâm nghiệpbáo cáo khoa học thủy sảnBáo cáo thực tập tại nhà thuốc tại Thành phố Hồ Chí Minh năm 2018chuyên đề điện xoay chiều theo dạngNghiên cứu tổ chức pha chế, đánh giá chất lượng thuốc tiêm truyền trong điều kiện dã ngoạiNghiên cứu tổ hợp chất chỉ điểm sinh học vWF, VCAM 1, MCP 1, d dimer trong chẩn đoán và tiên lượng nhồi máu não cấpBiện pháp quản lý hoạt động dạy hát xoan trong trường trung học cơ sở huyện lâm thao, phú thọGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitTrả hồ sơ điều tra bổ sung đối với các tội xâm phạm sở hữu có tính chất chiếm đoạt theo pháp luật Tố tụng hình sự Việt Nam từ thực tiễn thành phố Hồ Chí Minh (Luận văn thạc sĩ)Phát triển du lịch bền vững trên cơ sở bảo vệ môi trường tự nhiên vịnh hạ longNghiên cứu về mô hình thống kê học sâu và ứng dụng trong nhận dạng chữ viết tay hạn chếNghiên cứu khả năng đo năng lượng điện bằng hệ thu thập dữ liệu 16 kênh DEWE 5000Định tội danh từ thực tiễn huyện Cần Giuộc, tỉnh Long An (Luận văn thạc sĩ)Tìm hiểu công cụ đánh giá hệ thống đảm bảo an toàn hệ thống thông tinSở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIXGiáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtHIỆU QUẢ CỦA MÔ HÌNH XỬ LÝ BÙN HOẠT TÍNH BẰNG KIỀMTÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲ