Báo cáo khoa học: Interaction of gymnemic acid with cyclodextrins analyzed by isothermal titration calorimetry, NMR and dynamic light scattering doc
Báo cáo khoa học: Interaction of gymnemic acid with cyclodextrins analyzed by isothermal titration calorimetry, NMR and dynamic light scattering doc
... Interaction of gymnemic acid with cyclodextrins analyzed by isothermal titration calorimetry, NMR and dynamic light scattering Yusuke Izutani1, Kenji Kanaori2, Toshiaki Imoto3 and Masayuki ... effect of gymnemic acid (GA) is diminished by application of c-cyclodextrin (c-CD) to themouth was evaluated at the molecular level using isothermal titration calorimetry, NMR and dynamic light scattering. ... the aggregate property of GA and the inclusion complexation of GA by c-CD.AbbreviationsCD, cyclodextrin; DLS, dynamic light scattering; GA, gymnemic acid; ITC, isothermal titration calorimetry.6154...
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Báo cáo khoa học: Interaction of caspase-3 with the cyclic GMP binding cyclic GMP specific phosphodiesterase (PDE5a1) potx
... inactivation of PDE5A1 by caspase-3 mightoccur via removal of key regions which constitute part of the wall of the catalytic site of PDE5A1. We also suggesta possible interaction between PDE5A1 and ... Institute of Biological and Life Sciences, University of Glasgow, Scotland;5Department of Molecular Biology, Institute of Biotechnology, Flanders Interuniversity, University of Ghent, BelgiumHere, ... Houslay4,Peter Vandenabeele5 and Nigel J. Pyne11Department of Physiology and Pharmacology, Strathclyde Institute for Biomedical Sciences, University of Strathclyde, Glasgow,Scotland;2Department of...
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Báo cáo khoa học: Interaction of selenium compounds with zinc finger proteins involved in DNA repair pdf
... Food Chemistry and Toxicology, University of Karlsruhe, Germany;2Institute of Biochemistry and Biophysics, PolishAcademy of Sciences, Warsaw, Poland;3Institute of Food Technology and Food Chemistry, ... IIÞwhere N ¼ the number of s trand breaks per molecule of PM2, I ¼ the p ercentage of supercoiled PM2 DNA, and II ¼ the percentage of open circular PM2 DNA. Theoverall number of s trand breaks p er ... demonstrated by CD spectra and fluorescence spectroscopy [31].Unbound zinc and salts were removed from MT by ultrafiltration and MT was further characterized by quan-tification of the thiol groups with...
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Báo cáo khoa học: Role of the C-terminal extension in a bacterial tyrosinase Michael Fairhead and Linda Thony-Meyer doc
... reconstituted with copper by mixing an aliquot of protein ( 10 mg) with an equal vol-ume of 10 mm Tris-HCl (pH 8), containing a three-foldmolar excess of CuSO4in a final volume of 1 mL. The ... m)1Æcm)1.TrypsinizationTrypsin digest of purified pro-tyrosinase was performed by dissolving 20 lg of proteomics grade TPCK treated porcinetrypsin (Sigma-Aldrich) in 50 lLof1mm HCl and mixingit with an aliquot of pro-tyrosinase ... Determination of the mass of the proteins by MS revealed masses of 36 506 kDa (recombinant coretyrosinase) and 37 874 kDa (trypsinized pro-tyrosi-nase) corresponding to a C-terminal amino acid of Val357...
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Tài liệu Báo cáo khoa học: Interaction of the small heat shock protein with molecular mass 25 kDa (hsp25) with actin doc
... HSP25 on the polymerization of intact actin and aggregation of heated actin. Interaction of HSP25 with intact actinIn the first series of experiments we analyzed the effect of HSP25 on the apparent ... buffer G and wasinitiated by the addition of KCl and MgCl2up to 50 mM and 2 mM, respectively. Different quantities of actin nuclei(short fragments of F-actin) were added simultaneously with KCl ... possibility we heated actin containing10% of pyrene-labeled protein for 1 h at 43 and 60 °Cand analyzed polymerization and aggregation induced by theaddition of KCl and MgCl2(Fig. 5). As expected,...
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Báo cáo khoa học: Interaction of the general transcription factor TnrA with the PII-like protein GlnK and glutamine synthetase in Bacillus subtilis potx
... S3) [10,19]. Interactions of the truncatedTnrA proteins with GlnK and with GS were determined by pulldown and SPR analysis, as described above(Fig. 6). As expected, the C-terminus of TnrA was ... deletion of even sixamino acids abolished this interaction (Fig. 6A,B).Truncated forms of TnrA with the C-terminus lackingsix or 20 amino acids still bound to GlnK; however,removal of 35 amino acids ... analyzed by western blotting.Fig. 6. The interaction of truncated TnrA proteins with GlnK and GS. (A) BIAcore analysis of GlnK and GS binding to wild-type TnrA(TnrAwt), TnrA6, TnrA20, and TnrA35....
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Báo cáo khoa học: Interaction of synthetic peptides corresponding to hepatitis G virus (HGV/GBV-C) E2 structural protein with phospholipid vesicles doc
... ShaiY & Jelinek R (2002) Structures and mode of mem-brane interaction of a short alpha helical lytic peptide and its diastereomer determined by NMR, FTIR, and fluorescence spectroscopy. Eur J ... of lipids, were recorded between310 nm and 450 nm, with an excitation wavelength of 290 nm, at a slit width of 4 nm. The fluorescence spectrawere instrument corrected for light scattering, by ... membranefusion step. However, the nature of the interaction of viral fusion proteins with membranes and the mechan-ism by which these proteins accelerate the formation of membrane fusion intermediates...
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Báo cáo khoa học: Interaction of human stefin B in the prefibrillar oligomeric form with membranes Correlation with cellular toxicity doc
... oligomers of vesicles made of acidic phospholipids resembles poreformation by A-b [27] and liposome permeabilization of a-synuclein [43]. The toxic activity exerted by prefi-brillar forms of stefin B and ... by both transmis-sion electron microscopy (TEM) and atomic forcemicroscopy [15].In this study, we measured the interaction of stefin B with various combinations of phospholipid monolayers and ... (pH 7.3) and to prefibrillar aggregates of stefin B, both, atpH 4.8 and 3.3. Protein concentration in each case was 22 lM (light bar) and 41 lM (dark bar). Values shown are averages of five inde-pendent...
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Báo cáo khoa học: Interaction of Sesbania mosaic virus movement protein with the coat protein – implications for viral spread Soumya Roy Chowdhury and Handanahal Subbarao Savithri docx
... deletion of one, two and three pre-dicted helices from the N-terminus of MP reduced the interaction with NV by 51.5%, 66.4% and 80.1%,respectively, compared with the interaction betweenGST–MP and ... 4. Interaction of rMP with NV. (A) ELISA of rMP and NV inter-action. ELISA plates coated with NV (5 lg) (P1) were blocked with 10% skimmed milk in 1% NaCl ⁄ Pi (block) followed by addition of 5 ... together with 50 lL aliquots of unbound and wash fractions, were separated by SDS ⁄ PAGE followed by silver staining to check for the presence of proteins.The interaction between MP, MP mutants and...
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Báo cáo khoa học: Interaction of G-rich GT oligonucleotides with nuclearassociated eEF1A is correlated with their antiproliferative effect in haematopoietic human cancer cell lines potx
... contaminant of eEF1A protein can be excluded by MALDI TOF analysis of the Coomassie blue bandextract [21] and by the absence of a correspondingnucleolin signal in western-blotting of our nuclearextracts ... werewashed twice with buffer C and twice with buffer C with- out NP40. The elution was made with three volumes of buffer C without NP40 adjusted to 1.5 m NaCl, incubatingfor 10 min with gentle stirring. ... steps of freeze ⁄ thawing, followed by precipitation with cold acetone, the protein was rinsed with methanol,denatured with 8 m urea and then renatured by overnightincubation in a fixed volume of...
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