Báo cáo khoa học: Secondary structure assignment of mouse SOCS3 by NMR defines the domain boundaries and identifies an unstructured insertion in the SH2 domain pdf
... degradation of the protein.
The latter half of the kinase inhibitory region and the entire extended SH2
subdomain form a single a-helix. The mapping of the true SH2 domain,
and the location of its ... within SOCS3.
The PEST sequence is an insertion in the SH2
domain
Analysis of the secondary structure of SOCS3, and
sequence alignments...
... mea-
sured in solution. Furthermore, it resembles the mean
of an ensemble of 20 structures and thus indicates the
dynamics of the protein, leading to changes in second-
ary structure according to the ... [19]. Therefore, the error of
the secondary structure change is much lower
than the error of the absolute secondary structure
determination.
The...
... and and in the 20±25 loop, as well as in the P2¢ and P 3¢
positions of the binding loop. The residues with the greatest
Dd
NH
values are Thr16 and, more interestingly, Gln35. The
majority of the ... equential, and long-range (in cluding medium-range) NOE di stance restraints per residue for SGCI (A) and SGTI (B);
angular order parameters [26] of / and w tor...
... production of VP2 was prepared
by insertion of amplified sequences into the HindIII and
BglII sites, and the plasmid for production of VP3 was pre-
pared by insertion of amplified sequences into BglII and
KpnI ... test interac-
tions of the minor structural proteins in cells, contribu-
tions of individual hydrophobic domains to their
membrane affinity and to r...
... (1997)
Anticoagulant synergism of heparin and activated protein C in
vitro. Role of a novel anticoagulant mechanism of heparin,
enhancement of inactivation of factor V by activated protein C.
J. Clin. ... constants of FVa inactivation as
UFH, but pentasaccharide did not inhibit FVa inactivation.
Analysis of these data in the context of the 3D structures of
APC a...
... significance of
the recombinant S. lividans cells expressing the
R73V ⁄ R84A variant from the viewpoint of industrial
and medical applications.
Results
Expression of the R73V
⁄
R84A variant of
CYP105A1 ... Wat560, 587
and 593 in the binding site were included in the calculation.
Cluster analysis was performed on the docked results of
100 runs using a root-mean-s...
... different plant and animal sources, with more than 400
isoforms being obtained from amphibian species. This
article discusses the antibacterial activity and mode of
action of the N-terminal region of ... different initial
surface pressures (p
0
), the peptide (0.1–2 lm) was injected
into the subphase, and the increment in surface pressure of
the LPS film upon interc...
... isomers of cyclosarin
[30] and those of p-nitrophenol analogs of sarin and
soman [25]. Therefore, it was of interest to examine
the stereoselectivity of cyclosarin and soman hydro-
lysis exerted by ... constants
(k
cat
⁄ K
m
)of1 0
5
)10
6
m
)1
Æmin
)1
[16]. The catalytic effi-
ciency of PON1 in the hydrolysis of sarin and soman
and the possibility to re-in...
... hydrolysis, and improving the
Fig. 1. (A) The crystal structure of TaLcc1
as a surface model. Domain A is presented
in blue, domain B in green, and domain C in
yellow. The N-glycans are shown ... Asn555. The
glycan on Asn202 was in the groove between the b-bar-
rels of domains A and B (Fig. 2B), and had six hydro-
gen bonds, three to the main chain ca...
... cargo binding sites, referred to as
the major and minor binding sites [1]. These sites are
located in the concave face of the protein near regions
of invariant Trp and Asn arrays. The major binding
site ... to the flexible importin-b binding domain. The
importin-b binding domain is known to have an
autoinhibitory function, whereby an internal NLS-like
sequence comp...