Báo cáo khoa học: Kinetic deuterium isotope effects for 7-alkoxycoumarin O-dealkylation reactions catalyzed by human cytochromes P450 and in liver microsomes Rate-limiting C-H bond breaking in cytochrome P450 1A2 substrate oxidation pdf

... Kinetic deuterium isotope effects for 7-alkoxycoumarin O-dealkylation reactions catalyzed by human cytochromes P450 and in liver microsomes Rate-limiting C-H bond breaking in cytochrome P450 ... similar for 7-OMe and 7-OEt coumarin, with P450 1A2 showing high isotope effects (8–16) and P450 2E1 yielding isotope effects of 2–4, allowing for the variability in both cases. For P450 2E1, ... (OEt) coumarin has been used as a model substrate in many cyto-chrome P450 (P450) studies, including the use of kinetic isotope effects toprobe facets of P450 kinetics. P450s 1A2 and 2E1 are...

Tài liệu Báo cáo khoa học: Analysis of the molecular dynamics of medaka nuage proteins by ﬂuorescence correlation spectroscopy and ﬂuorescence recovery after photobleaching doc

... proteins [13] and binding interactions [14].FRAP is a conventional technique used to study the kinetic properties of proteins in a cell by measuringthe ﬂuorescence recovery rate in a bleached ... dynamic properties of proteins in the PGC determined by FCS and FRAP. A fusionprotein consisting of Olvas and green ﬂuorescent pro-tein (GFP) (Olvas–GFP) expressed in the PGC formsgranules that exhibit ... microinjected into the medaka eggs and transfected into HeLa cells. The olvas, nanos and tudorcoding sequences were joined to the C-terminal region of the GFP or RFP coding sequence in an in- frame...

Báo cáo khoa học: Modeling of ATP–ADP steady-state exchange rate mediated by the adenine nucleotide translocase in isolated mitochondria potx

... (A).Inset: a representative experiment showing the effect of the addi-tion of cATR (in the concentrations indicated in the inset ﬁgure, in nM)onDWm, as indicated in the inset of (B). Data in ... activity in rat liver mito-chondria. J Biol Chem 265, 8444–8450.61 Austin J & Aprille JR (1984) Carboxyatractyloside-insensitive in ux and efﬂux of adenine nucleotides in rat liver mitochondria. ... Adenosinediphosphate translocation in mitochondria. Nature ofthe receptor site for carboxyatractyloside (gummiferin).Biochemistry 12, 1508–1519.8 Demin O & Goryanin I (2008) Kinetic Modelling...

Tài liệu Báo cáo khoa học: Kinetic analysis of effector modulation of butyrylcholinesterase-catalysed hydrolysis of acetanilides and homologous esters pdf

... carboxyles-terases and albumin, was controlled by nondenaturingPAGE and activity staining [26,68].Kinetics of substrate hydrolysis in the absenceof ligand Kinetic assays with neutral and positively and negativelycharged ... actual binding site of serotonin cannot be inferredfrom these results. Although serotonin can bind to theactive site binding locus (W82), binding to PAS cannotbe ruled out. If serotonin binds ... & Rypins EB (1979) Tyra-mine kinetics and metabolism in cirrhosis. J Clin Invest64, 413–420.64 Boutin JA, Audinot V, Ferry G & Delagrange P (2005)Molecular tools to study melatonin pathways...

Tài liệu Báo cáo khoa học: Kinetic characterization of the ﬁrst step of the ribozyme-catalyzed trans excision-splicing reaction docx

... rate of substrate cleavage.Values for kobswere obtained from single-turnover reactions at44 °C using 166 nM ribozyme, 1.3 nM 5¢-end labeled substrate in buffer containing 135 mM KCl and 10 ... solutionof 300 nm ribozyme in 10 lL H10Mg buffer containing3.4% glycerol was preincubated for 5 min at 60 °C and P. P. Dotson II et al. Kinetics of the trans excision-splicing reactionFEBS Journal ... 5273–5281.12 Pyle AM & Green JB (1994) Building a kinetic frame-work for group II intron ribozyme activity: quantitationof interdomain binding and reaction rate. Biochemistry33, 2716–2725.13...

Tài liệu Báo cáo khoa học: Kinetic basis for linking the ﬁrst two enzymes of chlorophyll biosynthesis doc

... Kinetic basis for linking the ﬁrst two enzymes ofchlorophyll biosynthesisMark Shepherd, Samantha McLean and C. Neil HunterRobert Hill Institute for Photosynthesis and Krebs Institute for ... implying apossible role for this protein in substrate delivery.The next step in chlorophyll biosynthesis, catalysed by magnesium protoporphyrin IX methyltransferase(ChlM in Synechocystis), involves ... catalysis by accelerating the formation and breakdown of thecatalytic intermediate, providing a kinetic link betweenthe ﬁrst two reactions of chlorophyll biosynthesis, withthe signalling molecule...

Báo cáo khoa học: Kinetic and mechanistic characterization of Mycobacterium tuberculosis glutamyl–tRNA synthetase and determination of its oligomeric structure in solution pptx

... to A. Mattevi and M. Nardini for carrying outcrystallization trials, to G. Tedeschi and A. Negri for performing MALDI-TOF analyses and N-terminalsequencing, and to G. Deho` for helpful discussions.PVK, ... in the 20 lLaliquot (in nmol).Steady-state kinetic analyses and inhibition studiesThe apparent kcat and KMvalues of Mt-GluRS for tRNAGlu,ATP and l-Glu were determined in the tRNA aminoacyla-tion ... minor, implying rotations of domains ofjust a few degrees (e.g. 7° interdomain rotations upontRNA binding to the Tt-GluRS and local limitedrearrangements in the active site) [5]. Interestingly,...

Báo cáo khoa học: Kinetic characterization of methionine c-lyases from the enteric protozoan parasite Entamoeba histolytica against physiological substrates and triﬂuoromethionine, a promising lead compound against amoebiasis ppt

... 7.0) containing1mm dithiothreitol and 20 lm PLP, and 3–60 lgÆmL)1ofthe recombinant enzymes, at 37 °C for 10 min. The rangesof substrate concentrations for the measurement of kinetic parameters ... containing 20 lm PLP and 0.1%Triton X-100. The insoluble materials were eliminated by centrifugation at 15 000 g for 10 min, and subsequently12.4 and 1.24 mgÆmL)1of the supernatant was incubatedwith ... designed against methionine gamma-lyase-containing pathogens, has efﬁcacy in vitro and in vivo against Trichomonas vaginalis. Antimicrob AgentsChemother 45, 1743–1745.27 Inoue H, Inagaki K,...

Báo cáo khoa học: Kinetic properties of catecholoxidase activity of tarantula hemocyanin pot

... of enzyme kinetics, the conditions with constantactivity were established. This was accomplished by preincubating hemocyanin with 5 mm SDS for 5 minbefore adding the substrate. Using this condition,which ... assayed in 0.1M phosphate buffer (pH 7.0). Before initiation of the reaction by the addition of the substrate dopamine, hemocyaninwas preincubated for 5 min with 5 mM SDS and the indicated ... substrate. First,hemocyanin was preincubated with SDS for 5 min, and then thereaction was started by the addition of substrate. The reaction wasfollowed for 5 min by measuring the absorption at...

Báo cáo khoa học: Kinetic and binding studies with purified recombinant proteins ferredoxin reductase, ferredoxin and cytochrome P450 comprising the morpholine mono-oxygenase from Mycobacteriumsp. strain HE5 ppt

... recombinantproteins ferredoxin reductase, ferredoxin and cytochrome P450 comprising the morpholine mono-oxygenase fromMycobacterium sp. strain HE5Bernhard Sielaff and Jan R. AndreesenInstitut ... Fe3S4ferredoxin (Fdmor) and cytochrome P450 (P450 mor). In this study, we puriﬁed Fdmor and P450 moras recombinant proteins as well as FdRmor, which has been isolated previ-ously. Kinetic investigations ... active form [15]. NHis -P450 morcouldnow be puriﬁed in a stable form without detectableformation of the inactive P420 species.The binding of substrates to cytochromes P450 usu-ally induces...

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