Báo cáo khoa học: Site-specific phosphorylation of MCM4 during the cell cycle in mammalian cells pot
Báo cáo khoa học: Site-specific phosphorylation of MCM4 during the cell cycle in mammalian cells pot
... re-replication of DNA in a single cell cycle. Inactivation of CDK1 leadsto re-replication of DNA in eukaryotic cells includinghuman cells [7]. Targets of the kinase in the regulation of DNA replication include ... HeLa cell cycle in a site-specific manner.Cyclin-dependent protein kinase is involved in the phosphorylation of MCM4 To determine which kinase is involved in the phos-phorylation of MCM4 in phases ... enriched in the nucleolus through the cell cycle. Theseresults suggest that phosphorylation of MCM4 has several distinct and site-specific roles in the function of MCM during the mammalian cell cycle. AbbreviationsCDK,...
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Báo cáo khoa học: Functionally different pools of Shiga toxin receptor, globotriaosyl ceramide, in HeLa cells doc
... and the change of the molecular species composition of the cel-lular Gb3[28,29]. In in vitro binding assays, the fattyacid chain of Gb3was found to in uence the bindingto Shiga toxin [30,31]. In ... transport of the holotoxin and the delivery of the monomeric catalytic A-subunit into the cytosol, leading to the inhibition of protein biosynthe-sis [13,14]. In numerous cell lines [15], it has been ... 5% of those in untreated control cells. Under these conditions, the 4 °C binding protocol used for control cells does notallow detectable amounts of STxB to associate with cells. Therefore, the...
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Tài liệu Báo cáo khoa học: Insulin-dependent phosphorylation of DPP IV in liver Evidence for a role of compartmentalized c-Src ppt
... endocytosis [16]. In Madin–Darby canine kidney (MDCK) cells, a study of chimeric forms of DPP IV has shown that the luminaldomain of DPP IV carries dominant apical sortinginformation while the short ... signalobserved 15 min following insulin injection (Fig. 2A,lower panel).We then verified DPP IV phosphorylation in vitro.Following incubation of the nonsolubilized G ⁄ Efraction in the presence of [c-32P]ATP, ... min, but not at 15 minpostinsulin injection (Fig. 3A). In order to link this phosphorylation event with c-Src catalytic activity,samples were incubated either in the presence orabsence of the...
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Báo cáo khoa học: Specific cleavage of the DNase-I binding loop dramatically decreases the thermal stability of actin pot
... reveal the changes in the thermal unfolding of actin induced byinteraction of G-actin with actin-binding proteins [25–27], G–F transformation of actin, and stabilization of F-actin by phalloidin ... on the conformation of the nucleotide-binding cleft. This suggestion is sup-ported by the studies on the effects of actin-bindingproteins on actin structure.Actin-binding proteins profilin ... phalloidin-induced closing of the nucleotide-binding cleft. In conclusion, the results obtained in the presentstudy suggest that the thermal stability of actin, regard-less of whether it is...
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Báo cáo khoa học: Specific degradation of H. pylori urease by a catalytic antibody light chain pptx
... mixing (lane 1). At 4 h of incubation (lane 2), the bands of partially dissociated urease (ambn) became faint aswell as the band of the b-subunit monomer. In contrast, the intensity of the ... HpU-9-L, again indicating the digestion by HpU-9-L was specific. In summary, we succeeded in the pre-paration of a catalytic antibody light chain capable of specifically digesting the b-subunit of H. ... cleavage sites of the urease byN-terminal amino-acid sequencing of the peptide frag-ments. From the band (1), a sequence of GLIVT wasdetected with the intensity of 2 pmol. As a minor scis-sile...
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Tài liệu Báo cáo khoa học: "Arabic Tokenization, Part-of-Speech Tagging and Morphological Disambiguation in One Fell Swoop" pdf
... chosenfor the input word in training.8 The ATB generates normalized forms of certain clitics and of the word stem, so that the resulting tokens are not simply the result of splitting the original ... use the no-tion of ambiguity class in the feature classifiers; in- stead we investigate different ways of using the re-sults of the individual feature classifiers in directlychoosing among the ... Proper Nounresulting from missing entries in the Buckwalter’slexicon. The remaining case was a failure in the conversion process relating to the handling of bro-ken plurals at the lexeme level....
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Báo cáo khoa học: Site specificity of yeast histone acetyltransferase B complex in vivo pot
... histoneloading, did not detect differences in the amount of histone H3 between the two strains, indicating that cells lacking Hat1 display normal levels of histones during the course of HU treatment.To ... treatment.To investigate whether HU induces an increase of the Hat1 protein, we used a yeast strain expressing ahemagglutinin (HA)-tagged version of Hat1. The Hat1–HA protein level did not increase ... acetylation of histone H4 was detected in the solu-ble fraction of cells at distinct cell cycle stages, and increased when cells accumulated excess histones. Strikingly, histone H3 was not found in anyof...
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Báo cáo khoa học: Actin-binding domain of mouse plectin Crystal structure and binding to vimentin pot
... contained in the N-terminal part of the molecule comprising the head domain and possiblyparts of the rod. The head domain of vimentin had previously been shownto harbor the binding site(s) for the ... (1995)Utrophin actin binding domain: analysis of actin binding andcellular targeting. J. Cell Sci. 108, 63–71.10. Gimona, M. & Winder, S.J. (1998) Single calponin homologydomains are not actin-binding ... functionalproperties of these ABDs. The ABD of plectin binds to vimentin The IF protein vimentin has been reported to specificallyinteract with the CH1 subdomain of the first (N-terminal) of fimbrin’s two...
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Báo cáo khoa học: Molecular dynamics structures of peptide nucleic acidÆDNA hybrid in the wild-type and mutated alleles of Ki-ras proto-oncogene ppt
... deciphering the origin of the destabilization and hence, diminution of the melting temperature (Tm) in the former.Base stacking in the vicinity of A C mismatch in PNAÆDNA and DNA duplexesIntra ... tointerrupted stacking, fluctuating nature of the hydro-gen bond and water organization in the vicinity of the mismatch might be the contributing factors for the increase in free energy and diminished stability ... interaction in the vicinity of A CmismatchFigure 7A–L depicts the nature of water interaction in the neighbourhood of A24 C7 mismatch in PDwt.Water interaction along the minor groove side of A24...
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Báo cáo khoa học: "A Comparative Study of Parameter Estimation Methods for Statistical Natural Language Processing" potx
... that the next point is in the valid region, we project each point during the line search back onto the chosen orthant.1 At each iteration, we choose the orthant containing the current point ... that the value of is adap-tively chosen according to the reduction of ExpLoss during training. The algorithm starts with a large initial , and then at each forward step the value of ... aggre-gates the decisions of local linear models via a dynamic program. In the CMM, the local linear models are trained independently, while in the CRF model, the local models are trained jointly....
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