Báo cáo khoa học: Mapping of the interaction site of CP12 with glyceraldehyde-3-phosphate dehydrogenase from Chlamydomonas reinhardtii Functional consequences for glyceraldehyde-3-phosphate dehydrogenase pot
... 3369
Mapping of the interaction site of CP12 with
glyceraldehyde-3-phosphate dehydrogenase from
Chlamydomonas reinhardtii
Functional consequences for glyceraldehyde-3-phosphate
dehydrogenase
Sandrine ... and the GAPDH substrate, BPGA.
To date, mapping of the interaction site of CP12
had not studied but our results give some clues with
rega...
... His145Asn
mutations on the affinity of metal binding
to arginase II
To further examine the effects of the His120fiAsn
and His145fiAsn mutations on the interaction of the
enzyme with manganese ions, ... addition to removal of the
more weakly bound Mn
2+
, the lower affinity for that
more tightly bound to the protein may explain the
absence of Mn
2+
from the ED...
... Trp425 of
Dex49A, which is the corresponding residue of Trp402 of
IPU, is reportedly located in the vicinity of the active site
and could form a binding site for a glucosyl unit i n subsite
)1 ... (Fig. 4B). Together with the observations from the
site- directed mutagenesis, Trp402 of IPU appears to be a
residue participating in subsite )1.
The r esidues that...
... between ,
, and the other measures, with the exception of
mere co-occurrence frequency.
Summing up, t-test – with a few exceptions
around the first 5% of the data in the SLs –
leads to the overall ... provides a base-
line for the interpretation of the precision curves.
General findings from the precision graphs are:
(i) It is only useful to consider the first h...
... decay
(for most of them obtained with tryptophan) and their
molecular masses (Fig. 10). We compared these data with
the results of calculations of their hydrodynamic properties
performed from their ... direct exploitation
of the structural results of the former to explain the
mechanism of CaM activation of the latter. Crystallization
and resolution of the 3...
... diminish
the immunoblot signals for bacitracin at the site of
PDI (Fig. 4C). These data support the view of a robust
covalent PDI–bacitracin interaction.
We hypothesized that the thiazoline ring of ... ion mode CID-TOF ⁄ TOF MS analysis of
the same precursor yielded the corresponding signature
peaks (m ⁄ z 1404.88, 1438.82 and 1470.80) of the other
part of the...
... consisting
of V
H
and V
L
regions with ⁄ without the V
H
leader
sequence at the N-terminal and with ⁄ without the
C
L
(j) region behind the V
L
region. None of the con-
structs contained the KDEL ... antigen. Therefore, large-scale
libraries and extensive screening are required for the
selection of the antigen-specific antibody fragments. On
the other hand, the...
... how the second kinase in the
cascade determines the time dependence of the activity
of the third kinase, we varied the V
max
of the second
kinase reaction and recalculated the concentration of
the ... are controlled by the kinases or phosphatases
in the system, and whether they are controlled differ-
entially by some or all of them. On the basis of the
an...
... the N domain of TnC binds
in a calcium-dependent manner to the C domain of TnI, releasing the
inhibitory effect of the latter on the actomyosin interaction. While the crys-
tal structure of the ... to the larger chimera. These results
are similar with the results observed for the binary
complexes formed by TnC(1–91) and TnI(1–147), and
TnC and TnI [16] and agr...
... ompatible
with the result on t he interactions of soluble fusion protein
fragments of Semliki Forest virus with membrane [10]. Thus
the i nsensitivity of the normalized intensity to variation in
the ... rate of
50 nm Æmin
)1
with a time constant of 2 s, step resolution
of 0.1 nm, and bandwidth of 1 nm. The final spectra were
taken from the average of five s c...