Báo cáo khoa học: Mapping of the interaction site of CP12 with glyceraldehyde-3-phosphate dehydrogenase from Chlamydomonas reinhardtii Functional consequences for glyceraldehyde-3-phosphate dehydrogenase pot

Báo cáo khoa học: Mapping of the interaction site of CP12 with glyceraldehyde-3-phosphate dehydrogenase from Chlamydomonas reinhardtii Functional consequences for glyceraldehyde-3-phosphate dehydrogenase pot

Báo cáo khoa học: Mapping of the interaction site of CP12 with glyceraldehyde-3-phosphate dehydrogenase from Chlamydomonas reinhardtii Functional consequences for glyceraldehyde-3-phosphate dehydrogenase pot

... 3369 Mapping of the interaction site of CP12 with glyceraldehyde-3-phosphate dehydrogenase from Chlamydomonas reinhardtii Functional consequences for glyceraldehyde-3-phosphate dehydrogenase Sandrine ... and the GAPDH substrate, BPGA. To date, mapping of the interaction site of CP12 had not studied but our results give some clues with rega...
Ngày tải lên : 16/03/2014, 13:20
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Tài liệu Báo cáo khoa học: Insights into the interaction of human arginase II with substrate and manganese ions by site-directed mutagenesis and kinetic studies Alteration of substrate specificity by replacement of Asn149 with Asp docx

Tài liệu Báo cáo khoa học: Insights into the interaction of human arginase II with substrate and manganese ions by site-directed mutagenesis and kinetic studies Alteration of substrate specificity by replacement of Asn149 with Asp docx

... His145Asn mutations on the affinity of metal binding to arginase II To further examine the effects of the His120fiAsn and His145fiAsn mutations on the interaction of the enzyme with manganese ions, ... addition to removal of the more weakly bound Mn 2+ , the lower affinity for that more tightly bound to the protein may explain the absence of Mn 2+ from the ED...
Ngày tải lên : 20/02/2014, 02:21
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Tài liệu Báo cáo khoa học: nsights into the reaction mechanism of glycosyl hydrolase family 49 Site-directed mutagenesis and substrate preference of isopullulanase doc

Tài liệu Báo cáo khoa học: nsights into the reaction mechanism of glycosyl hydrolase family 49 Site-directed mutagenesis and substrate preference of isopullulanase doc

... Trp425 of Dex49A, which is the corresponding residue of Trp402 of IPU, is reportedly located in the vicinity of the active site and could form a binding site for a glucosyl unit i n subsite )1 ... (Fig. 4B). Together with the observations from the site- directed mutagenesis, Trp402 of IPU appears to be a residue participating in subsite )1. The r esidues that...
Ngày tải lên : 19/02/2014, 16:20
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Tài liệu Báo cáo khoa học: "Methods for the Qualitative Evaluation of Lexical Association Measures" doc

Tài liệu Báo cáo khoa học: "Methods for the Qualitative Evaluation of Lexical Association Measures" doc

... between , , and the other measures, with the exception of mere co-occurrence frequency. Summing up, t-test – with a few exceptions around the first 5% of the data in the SLs – leads to the overall ... provides a base- line for the interpretation of the precision curves. General findings from the precision graphs are: (i) It is only useful to consider the first h...
Ngày tải lên : 20/02/2014, 18:20
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Báo cáo khóa học: Insight into the activation mechanism of Bordetella pertussis adenylate cyclase by calmodulin using fluorescence spectroscopy pptx

Báo cáo khóa học: Insight into the activation mechanism of Bordetella pertussis adenylate cyclase by calmodulin using fluorescence spectroscopy pptx

... decay (for most of them obtained with tryptophan) and their molecular masses (Fig. 10). We compared these data with the results of calculations of their hydrodynamic properties performed from their ... direct exploitation of the structural results of the former to explain the mechanism of CaM activation of the latter. Crystallization and resolution of the 3...
Ngày tải lên : 07/03/2014, 15:20
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Báo cáo khoa học: Bacitracin inhibits the reductive activity of protein disulfide isomerase by disulfide bond formation with free cysteines in the substrate-binding domain pptx

Báo cáo khoa học: Bacitracin inhibits the reductive activity of protein disulfide isomerase by disulfide bond formation with free cysteines in the substrate-binding domain pptx

... diminish the immunoblot signals for bacitracin at the site of PDI (Fig. 4C). These data support the view of a robust covalent PDI–bacitracin interaction. We hypothesized that the thiazoline ring of ... ion mode CID-TOF ⁄ TOF MS analysis of the same precursor yielded the corresponding signature peaks (m ⁄ z 1404.88, 1438.82 and 1470.80) of the other part of the...
Ngày tải lên : 14/03/2014, 23:20
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Báo cáo khoa học: Intrabodies against the EVH1 domain of Wiskott–Aldrich syndrome protein inhibit T cell receptor signaling in transgenic mice T cells potx

Báo cáo khoa học: Intrabodies against the EVH1 domain of Wiskott–Aldrich syndrome protein inhibit T cell receptor signaling in transgenic mice T cells potx

... consisting of V H and V L regions with ⁄ without the V H leader sequence at the N-terminal and with ⁄ without the C L (j) region behind the V L region. None of the con- structs contained the KDEL ... antigen. Therefore, large-scale libraries and extensive screening are required for the selection of the antigen-specific antibody fragments. On the other hand, the...
Ngày tải lên : 16/03/2014, 14:20
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Báo cáo khoa học: Principles behind the multifarious control of signal transduction ERK phosphorylation and kinase/phosphatase control potx

Báo cáo khoa học: Principles behind the multifarious control of signal transduction ERK phosphorylation and kinase/phosphatase control potx

... how the second kinase in the cascade determines the time dependence of the activity of the third kinase, we varied the V max of the second kinase reaction and recalculated the concentration of the ... are controlled by the kinases or phosphatases in the system, and whether they are controlled differ- entially by some or all of them. On the basis of the an...
Ngày tải lên : 16/03/2014, 18:20
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Báo cáo khoa học: Mapping contacts between regulatory domains of skeletal muscle TnC and TnI by analyses of single-chain chimeras potx

Báo cáo khoa học: Mapping contacts between regulatory domains of skeletal muscle TnC and TnI by analyses of single-chain chimeras potx

... the N domain of TnC binds in a calcium-dependent manner to the C domain of TnI, releasing the inhibitory effect of the latter on the actomyosin interaction. While the crys- tal structure of the ... to the larger chimera. These results are similar with the results observed for the binary complexes formed by TnC(1–91) and TnI(1–147), and TnC and TnI [16] and agr...
Ngày tải lên : 16/03/2014, 18:20
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Tài liệu Báo cáo khoa học: Structure and membrane interaction of the internal fusion peptide of avian sarcoma leukosis virus pdf

Tài liệu Báo cáo khoa học: Structure and membrane interaction of the internal fusion peptide of avian sarcoma leukosis virus pdf

... ompatible with the result on t he interactions of soluble fusion protein fragments of Semliki Forest virus with membrane [10]. Thus the i nsensitivity of the normalized intensity to variation in the ... rate of 50 nm Æmin )1 with a time constant of 2 s, step resolution of 0.1 nm, and bandwidth of 1 nm. The final spectra were taken from the average of five s c...
Ngày tải lên : 19/02/2014, 16:20
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