Báo cáo khoa học: Characterization of N-glycosylation consensus sequences in the Kv3 1 channel pot

Báo cáo khoa học: Characterization of N-glycosylation consensus sequences in the Kv3.1 channel pot

... SGEYCFig. 1. Topological model of Kv3. 1 andamino acid sequences of the S1–S2 linker of Kvs. (A) Topology of a Kv3. 1 monomericunit. Black circles represent the Asn of native N-glycosylation consensus ... that N-glycosylation in uences the open-ing of the voltage-dependent gate of Kv3. 1, suggest-ing that vacant sites alter the folding and maturation of Kv3. 1 at the cell surface.NC220229S1 ... unglycosylat-ed Kv3. 1s expressed in Sf9 cells (Fig. 7A,B) butoccurred less often than the inactivating currents. In the case of the noninactivating behavior, the channel densities for wild-type Kv3. 1 (Imax⁄...

Báo cáo khóa học: Characterization of novel structural features in the lipopolysaccharide of nondisease associated nontypeable Haemophilus inﬂuenzae pptx

... GlycoformObserved Calculated 11 -OS -1 11- OS-2 11 -OS-3 16 -OS -1 1263.7 12 40.7 12 40.6 1 2 2 6 Hex 1 •Hep3•AnKdo-ol Hex1 14 67.8 14 44.8 14 44.7 7 18 19 9 Hex2•Hep3•AnKdo-ol Hex2 16 71. 9 16 48.9 16 48.8 ... responseLinkage assignmentLPS-OH(strain 11 )LPS-OH(strain 16 ) 11 -OS -1 11- OS-1HF 11 -OS-2 11 -OS-3 16 -OS-1HF 16 -OS-22,3,4,6-Me4-Glc 1. 00 23 26 31 33 30 37 26 42D-Glcp- (1- 2,3,4,6-Me4-Gal 1. 04 1 ... minor fractions, 11 -OS -1, 11 -OS-2 and 11 -OS-3 for strain 11 and one major and oneminor fraction for strain 16 , 16 -OS -1 and 16 -OS-2. The major oligosaccharides 11 -OS -1/ 16-OS -1 were dephospho-rylated...

Tài liệu Báo cáo khoa học: Characterization of electrogenic bromosulfophthalein transport in carnation petal microsomes and its inhibition by antibodies against bilitranslocase docx

... values (nmolÆmin )1 Æmg protein )1 )ATPase None 2 41 437 393400 lM Na3VO4 18 1 10 6 335 10 0 nM baﬁlomycin 18 7 412 13 1 lgÆmL )1 oligomycin 16 3 418 325Cytochrome c reductase – 15 8 15 32Latent ... cyanidin; 10 0 nm bilirubin and 10 0 nm biliverdin. Under the condi-tions of the assay, bilirubin is freely soluble in the buffer[58]. The presence of these inhibitors in the assay mediummay interfere ... the text and in Fig. 5B. n, Number of [A] tested; k2⁄ k 1 , the value of the intercept in the Scruttonand Utter plot, where k2and k 1 are the rate constants of the inhibition of either the...

Tài liệu Báo cáo khoa học: Characterization of ICAM-4 binding to the I domains of the CD11a/CD18 and CD11b/CD18 leukocyte integrins pptx

... preparations of CD11b/CD18 integrins. The mAbs to CD11a/CD18, CD11b/CD18and ICAMs clearly inhibited the binding of ICAM L cellsto coated CD18 integrins (data not presented).To examine the role of I ... domain in ICAM-2 seem to mediate CD11b/CD18 binding [12 ].Extensive work has been carried out to localize the ligandbinding sites in the leukocyte b2integrins. The a chains of the CD 11/ CD18 integrins ... domain GST fusion proteins of CD11a andCD11b (Figs 6 and 7). The CD11a I domain speciﬁcTS1/22 mAb efﬁciently inhibited the binding of ICAM -1 and -2 transfected L cells to the I domain of CD11a,...

Báo cáo khoa học: Characterization of a prokaryotic haemerythrin from the methanotrophic bacterium Methylococcus capsulatus (Bath) ppt

... identi-ﬁed all the candidate residues involved in the binding of diiron (His22,His58, Glu62, His77, His 81, His 117 , Asp122) and the amino acids forming the hydrophobic pocket in which O2may bind (Ile25, ... from the PCR fragmentampliﬁed with the primers used in the cloning of MCA0 715 . The arrowhead indicates the 540 bp transcript, corresponding to the predicted length of the MCA0 715 transcript. The ... McHr. The seven ligands of the diiron site in haemeryth-rins are amino acid residues each located within the four helices, and by diiron binding generating anintramolecular cross-linking of these...

Báo cáo khoa học: Characterization of a digestive carboxypeptidase from the insect pest corn earworm (Helicoverpa armigera) with novel speciﬁcity towards C-terminal glutamate residues pptx

... 2004, revised 19 March 2004,accepted 24 March 2004)Eur. J. Biochem. 2 71, 2000–2 011 (2004) Ó FEBS 2004 doi :10 .11 11/ j .14 32 -10 33.2004.0 411 3.xextracts of the webbing clothes moth, Tineola bisselliella.Soluble ... majority, and by 20 min all, of the original 50 kDa protein had been digested, with anincrease in staining of the bands of  36 kDa and 13 kDa. On further incubation with trypsin the 13 kDapolypeptide ... which determines substrate speciﬁcity byinteracting with the side chain of the P 1 ¢ residue. In the protein predicted by HaCA42 this residue is arginine. Thereare also two consensus N-glycosylation...

Tài liệu Báo cáo khoa học: Role of K22 and R120 in the covalent binding of the antibiotic fosfomycin and the substrate-induced conformational change in UDP-N-acetylglucosamine enol pyruvyl transferase docx

... K22 is missing (as in the K22Vmutant), the fosfomycin binding/reaction with C 115 occursonly in the presence of UDPNAG as the substrate promotes the interaction of R120 and fosfomycin in order ... concentration. The dissociation constants, Kdvalues, enthalpies of binding and stoichiometries weredetermined from the binding isotherm by ﬁtting a 1 : 1 binding model to the data using the software ... mutantproteins. This was interpreted as a lack of fosfomycinbinding to these mutant proteins [12 ]. Binding of fosfomycinto either wild-type MurA or the K22 mutant proteins in the absence of UDPNAG is not...

Báo cáo khoa học: Role of DptE and DptF in the lipidation reaction of daptomycin ppt

... 11 480.5apo-AcpK 10 5 61. 5 10 5 61. 0holo-AcpK 10 9 01. 6 10 9 01. 0decanoyl-DptF 13 986.9 13 986.9decanoyl-LipD 11 635.6 11 635.7decanoyl-AcpK 11 056.7 11 056.8M. Wittmann et al. Lipidation of ... ()) indicates the reaction without Sfp.m/z 13 4 91. 6apo-DptF+ Na+ KaRelative abundance 13 200 13 300 13 400 13 500 13 600 13 700 13 800 13 900 13 600 13 700 13 800 13 900 14 000 14 10 0 13 500Relative ... the proteins, substrates andproducts.[M+H]+(Da)Sample Mass observed Mass calculatedapo-DptF 13 4 91. 6 13 4 91. 9holo-DptF 13 8 31. 7 13 8 31. 9apo-LipD 11 14 0.2 11 14 0.4holo-LipD 11 480.4 11 ...

Báo cáo khoa học: Deamidation of labile asparagine residues in the autoregulatory sequence of human phenylalanine hydroxylase potx

... H4biopterinVmaxa(nmolÆmin )1 Æmg )1 )½S0:5a(lM)kcat/[S]0.5(lMÆmin )1 ) hSubstrateinhibitionVmax(nmolÆmin )1 Æmg )1 )Kmb(lM)Wt (2 h) 3484 ± 16 2 238 ± 18 0.72 1. 5 ± 0 .1 ‚ 5325 ± 13 7 31 ± 3Wt (24 h) 4946 ± 2 81 135 ± 11 1. 83 1. 9 ± 0 .1 + 6392 ... 310 7.470, 310 8.474 and 310 9.476) of decreasing intensity on deconvolution of the mass spectrum (Fig. 4E,F)7. This increase of 1 Da for each of the additional peaks corresponds to the increase in massas ... carbonyl of Asn by the nitrogen of the peptide group linking the Asn to the following residue [4–6]2. As hydrolysis of thisintermediate may occur on either side of the imidenitrogen, the Asp...

Báo cáo khoa học: Cooperation of two carotene desaturases in the production of lycopene in Myxococcus xanthus pot

... of the MR1 51- derived strain with the crtIa deletion (MR8 41) com-pletely lose the red color typical of colonies from the parental strain, suggesting that the absence of CrtIablocks the synthesis ... 4307 in- frame deletion of crtIa in M. xanthus strain MR1 51. This strain contains a mutation, carR3, which renders the expression of the carB operon independent of external stimulation [10 ,15 ]. ... genes of the carBoperon (Fig. 2) and a part of the fourth gene. PlasmidpMAR162 is similar to pMAR1 61 but with deletion of afragment encoding 16 0 amino acids of the coding region of crtIa. The M....

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