Báo cáo khoa học: In vitro embryonic developmental phosphorylation of the cellular nucleic acid binding protein by cAMP-dependent protein kinase, and its relevance for biochemical activities pdf
Báo cáo khoa học: In vitro embryonic developmental phosphorylation of the cellular nucleic acid binding protein by cAMP-dependent protein kinase, and its relevance for biochemical activities pdf
... November2006)doi:10.1111/j.1742-4658.2006.05596.x The zinc-finger cellular nucleic acid binding protein (CNBP) is a strikinglyconserved single-stranded nucleic acid binding protein essential for normalforebrain formation during mouse and ... 2006 The Authors Journal compilation ª 2006 FEBS 497 In vitro embryonic developmental phosphorylation of the cellular nucleic acid binding protein by cAMP-dependent protein kinase, and its relevance ... developmental expression of cellular nucleic acid- binding protein (CNBP) gene in Xenopus laevis. Gene 241, 35–43.21 Flink IL, Blitz I & Morkin E (1998) Characterization of cellular nucleic acid binding protein...
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Báo cáo khoa học: In vitro gamma-secretase cleavage of the Alzheimer’s amyloid precursor protein correlates to a subset of presenilin complexes and is inhibited by zinc potx
... [22–25]. Since a functioning zinc-bind-ing domain may be present in the Ab sequence of C99,we hypothesized that zinc may affect the oligomeriza-tion state of C101-3FLAG. Therefore, the molecularmass ... APPintracellular domain (AICD or e-CTF) by cleaving 9–7amino acids from c 40 and 42 sites at the e site [1–3].Several lines of evidence support the pathogenic role of c-cleavage of APP in Alzheimer’s ... starting methionine, the C-ter-minal 99 amino acids of APP and a C-terminal tripleFLAG tag. The resulting protein was named MC99-3FLAG (Fig. 1A). Escherichia coli expression vectorsencoding...
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Báo cáo khoa học: In vitro expansion of DNA triplet repeats with bulge binders and different DNA polymerases pdf
... T7, etc.) on the DNA exten-sion reaction by using32P-labeling primer or template in the presence or absence of DNA -binding agents(DDIs and doxorubicin). The DNA bulge binding of both compounds ... For example, the change in Tm(DTm) for the ATT bulge increased by 3.4 and 1.7 °C in the presence of DDI-1A and DDI-1B, respectively. The increase in DTm for DDI-1A was higher than that for ... tendency for repeatingDNA strands to form hairpin loops or slipped confor-mations, and their inherent conformational properties, for example their high degree of flexibility, writhing and the stability...
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Báo cáo khoa học: In vitro analysis of the relationship between endonuclease and maturase activities in the bi-functional doc
... statement and suggests that the RNA binding site is still available and its binding affinity is not altered by the presence of DNA in the DNA binding site.Interestingly, when 3 nMRNA and 10 nMDNA ... explain the complete inability of a saturating amount of DNA substrateto inhibit RNA splicing (Fig. 6B) and therefore it seemsreasonable to make the working hypothesis that the RNA and DNA binding ... of protein and three differentpreparations of DNA and RNA substrates. Each time the splicing reaction was minimally affected by prebinding the DNA to protein. As the concentration of protein...
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Báo cáo khoa học: In vitro and in vivo self-cleavage of Streptococcus pneumoniae signal peptidase I pot
... the protein into the targeting and translocatingpathway by interacting with the membrane and othercomponents of the cellular secretory machinery [1]. The signal peptides of secreted proteins ... demonstrated for the first time that the self-cleavage of SPase I is happening within the S. pneumoniae cells in all the growth phases, and the cells maintain the active SPase I at the highest level in the ... mem-brane by electroblotting. The membrane was then brieflystained by Commassie bright blue and destained by 50%methanol. The visualized protein bands on the membranewere excised and the N-terminal...
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Báo cáo khoa học: In vitro selection and characterization of a stable subdomain of phosphoribosylanthranilate isomerase potx
... g for 10 min. In vitro selection for mAb M2 binding was by adding 200-lL aliquots of the supernatant to the selection matrix and incubating at roomtemperature for 30 min. Unselected protein plasmid ... split- protein sensor for the detection of protein protein interactions [25]. In E. coli PRAI, the loop connecting b-strand 6 withhelix 6 (‘loop 6’) forms a long and flexible lid over the top of the active-site ... in rounds 2 and 3, and 20 of the 38identified in round 4). In every case, we observed aframeshift caused by insertion of a single thyminenucleotide into the fourth randomized codon of anotherwise...
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Báo cáo khoa học: In vitro characterization of a plastid terminal oxidase (PTOX) pot
... coli and studied its substrate preferences, the factors in uencing its activity and the nature of suitable metalcofactors and inhibitors in vitro. Materials and methodsChemicals The following ... [aliphatic-(X)3-H-(X)2/3-L/T/S] proposed by Fisher and Rich [20] for quinone binding sites. Althoughspeculative at this stage, this consensus sequence was found in various quinone -binding proteins including AOX [20,21].It ... probably the consequence of the increase in the overall quinone reducing activity of the membranes and not a direct effect on PTOX activity. This is corrobor-ated by the fact that the ratio of O2consumption...
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Báo cáo khoa học: O-MACS, a novel member of the medium-chain acyl-CoA synthetase family, specifically expressed in the olfactory epithelium in a zone-specific manner doc
... guanine nucleotide releasing protein ND – Rat IGF binding protein 5 ND –Rat FGF receptor activating protein 1 ND –Rat PKC delta -binding protein ND –Rat cellular retinoyl -binding protein ND ... proteinsRat RY2G5 potential ligand binding protein ND BG Rat RYF3 (vomeromodulin) ND LNGRat mucin-like protein 1,2,3 S Rat RYA3 potential ligand binding protein ND BGRat cocaine, amphetamine-regulated ... olfactory protein termed O-MACS, amember of the medium-chain acyl-CoA synthetase family. The o-macs gene encodes a protein of 580 amino acids,sharing 56–63% identity with other MACS family proteins.RT-PCR...
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Báo cáo khoa học: In situ proton NMR analysis of a-alkynoate biotransformations ppt
... sequencing, and protein biochemical characterization of the initialacetylene hydratase is inevitable. In case of the other, morecommon enzymes, which are involved a genomic sequenceanalysis and ... fromacetaldehyde are incorporated into the alcohol formation in the other metabolite. The assumption was proved by addingacetaldehyde (0.5 mM and 5 mM) to the biotransformation. The 0.5 mMamount ... characterization of the strainWe isolated a strain from rotten fruits by aerobic enrichmentcultures in liquid minimal medium containing 2-butynedio-ate as the sole carbon source. The bacterial strain wasclassified...
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Báo cáo khóa học: Nerve growth factor mediates activation of the Smad pathway in PC12 cells doc
... signaltransduction by interaction with cytoplasmic proteins suchas TAB1 [61] or mitogen-activated protein kinase kinasekinase [54]. These distinct antagonizing mechanisms of Smad7 open up the question whether ... Smad2 and Smad3 share 92% amino acid identity, they are functionally distinct. A short amino acid sequence in the MAD homology 1 (MH1) domain of Smad2 is responsible for its inability to bind DNA ... in multiple interdependent signalsbetween pathways originating from receptor serine/threo-nine kinases and receptor tyrosine kinases. Cellular responses induced by bone morphogenetic protein (BMP)...
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