Báo cáo khoa học: The common phospholipid-binding activity of the N-terminal domains of PEX1 and VCP/p97 doc

Tài liệu Báo cáo khoa học: DNA strand exchange activity of rice recombinase OsDmc1 monitored by ﬂuorescence resonance energy transfer and the role of ATP hydrolysis pptx

... betweendonor and acceptor dyes, will therefore unveil the sta-tus of physical union and separation of complementarystrands during renaturation and strand exchange,respectively, and thereby assesses the ... monitor the time course of renaturation and strand exchange activ-ities of OsDmc1. The renaturation activity was foundto be stimulated in the presence of ATP and was satur-ated at 1.25 lm of OsDmc1. ... observed,re-establishing the veracity of the assay to an ongoinghomologous strand exchange activity dependent onOsDmc1 function (Fig. 6, lines 1 and 6). The resultsindicated that the strand exchange activity...

Tài liệu Báo cáo khoa học:Symmetric fluoro-substituted diol-based HIV protease inhibitors Ortho-fluorinated and meta-fluorinated P1/P1¢-benzyloxy side groups significantly improve the antiviral activity and preserve binding efficacyy docx

... the antiviral activity and retained the binding efficacy. The flexibility of the target molecule complicates the prediction of an effectcaused by a modification on the inhibitor and necessitatesstructural ... surf ace and haspolarity d ifferences. This is documented by the threefoldimprovement in activity of inhibitors 2 and 6 against the triple mutant M46I, I82V and V84A compared with the nonsubstituted ... 2004Structure of the complexes The crystal structures of the six protease–inhibitor com-plexes have been solved and refined down to 1.8–2.3 A˚resolution with Rcryst and Rfree of 18.1–19.9% and 20.0–22.5%,...

Báo cáo khoa học: Relationship between functional activity and protein stability in the presence of all classes of stabilizing osmolytes ppt

... preferential hydration of the protein domain is the driving force of protein stabiliza-tion or folding [3,4]. Furthermore, the effect of osmolytes on the functional activity of an enzyme hasalso ... published elsewhere. We havetherefore measured the thermodynamic parameters of RNase-A in the presence of these amino acids and amino acid derivatives, and values of DGD°, measuredin triplicate, ... complexes [29]. On the other hand,kcat⁄ Kmis an apparent second-order rate constant thatrefers to the properties and the reaction of the freeenzyme and free substrate [29]. We have therefore esti-mated...

Báo cáo khoa học: Akt-dependent phosphorylation negatively regulates the transcriptional activity of dHAND by inhibiting the DNA binding activity pdf

... phosphorylation of dHANDdecreased the transcriptional a ctivity of dHAND/E47heterodimer.Fig. 6. The eﬀects of A kt expression on the transcriptional activity of dHAND. (A) AU5-d HAND, -dHAND-Ala-X, -dHAND-Ala, ... phosphorylation of these sites might affect the DNAbinding and/ or dimerization activities of dHAND. We ﬁrstanalyzed the effect of phosphorylation on the transcriptional activity of dHAND using reporter ... transcriptional activity of dHAND-WT/E47. The activity of dHAND-Ala-X/E47was p artially reduced by coexpression of Akt. But thisinhibitory effect of Akt was not detected in the case of dHAND-Ala/E47...

BÀI BÁO CÁO KHOA HỌC TẠI BẮC KINH ATCM 2009 THE SCIENTIFIC REPORT IN BEIJING - CHINA

... second number then we multiply two numbers together•Press the = key. Answer : 2.232710263 We continue to delete the digit 2 at the first number and the digit 7 at the second number then we multiply ... 1 The number contains the end two digits are digit 1 The number contains the end three digits are digit 1 The number contains the end four digits are digit 1Answer : the number contains the ... key and press 1 key6y⇒ =956 =5. Find the fourteenth digit after of the decimal point of We have : the digit 5 can have rounded We try the digit 5 can not have rounded We set : The second...

Tài liệu Báo cáo khoa học: A systems biology approach for the analysis of carbohydrate dynamics during acclimation to low temperature in Arabidopsis thaliana doc

... (black) and sucrose (grey). Doc. S1a. Model structure of C24. Doc. S1b. sbml format of model structure of C24. Doc. S2a. Model structure of Rsch. Doc. S2b. sbml format of model structure of Rsch. Doc. ... concentration cA(t) and the rate constant k. The model code and a detailed description of the modelstructure are provided in Docs S1a, S1b, S2a,b and S3). The identification of unknown parameters ... the maximum activity of the catalysingenzyme (Vmax,A) and the enzyme specific substrate affinity,expressed by Km,A. It also depends on the concentration of the reaction product and the dissociation...

Tài liệu Báo cáo khoa học: Mouse recombinant protein C variants with enhanced membrane afﬁnity and hyper-anticoagulant activity in mouse plasma pptx

... cooperativebinding of Ca2+turns the N-terminal part of the Gladomain inside out, exposing the hydrophobic x-loopto solvent and burying the majority of the Gla resi-dues. Of the seven Ca2+, the majority ... of bovine prothrombin fragment 1in complex with Ca2+[41], the 10 ⁄ 12 site resides near the x-loop and the linear array of bound calcium ions of the Gla domain. Indeed, the importance of the 10 ... the interaction to the cofactor and thus inﬂuence the anticoagulant activity of APC. An inﬂuence of position 23 on the interactionto APC substrates FVa and FVIIIa also cannot beruled out. The...

Tài liệu Báo cáo khoa học: KCNE4 can co-associate with the IKs (KCNQ1–KCNE1) channel complex ppt

... membrane. Together, our ﬁndings contributeto the understanding of the role of KCNE4 as apotentially important regulator of KCNQ1 and otherKVchannels.ResultsCharacterization of KCNE4 antibodyA ... KCNEproteins, and the overlapping expression patterns of these subunits [22], there may be multiple and diverseKCNE–KCNQ1 interactions within the same cells ortissues.One of the least characterized, ... the plasma mem-brane in the presence of KCNQ1. All lysates were probed with anti-HA to demonstrate the presence of KCNE4. (C) KCNQ1 trafﬁcs to the plasma membrane in the presence of KCNE1 and...

Tài liệu Báo cáo khoa học: Multiple promoters regulate tissue-speciﬁc alternative splicing of the human kallikrein gene, KLK11⁄hippostasin ppt

... RT-PCR to study the tissue-specific use of each promoter. We show the translation initiation site of isoform 2 and different intracellular distribution of isoforms 1 and 2, and discuss the regulatory ... part of isoform 2 mRNA. The thirdexon, exon 1c, extended from +1379 to +1454. The noncoding exons of exons 1a and 1c contributed to the 5¢ nontranslated region of isoform 1 mRNA in which the common ... the regulation and characteristics of these isoforms are unknown. We identified the first three exons (1a, 1b, and 1c) by determining their transcription initiation sites. Exon 1b con-tained the...

Tài liệu Báo cáo khoa học: Tryptophan tryptophylquinone cofactor biogenesis in the aromatic amine dehydrogenase of Alcaligenes faecalis Cofactor assembly and catalytic properties of recombinant enzyme expressed in Paracoccus denitriﬁcans pptx

... alog (pH )pKa2), Lim1 is the lower limit of the curve, Lim 2 is the middle limit of the curve and Lim 3 is the upper limit of the curve.Stopped-ﬂow kinetic studies of the oxidativehalf-reaction ... group on Trp57 of the smallsubunit to a carbonyl group, and (c) insert a secondoxygen atom into the TTQ ring [11]. The essential nat-ure of some of the genes in the mau gene cluster of P. denitriﬁcans ... and b subunits) inSDS ⁄ PAGE and migration is identical to thatobserved for the native enzyme. The migration of bothsubunits is consistent with the predicted masses of the mature form of the...

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