Báo cáo khoa học: Enzymatic oxidation of NADP+ to its 4-oxo derivative is a side-reaction displayed only by the adrenodoxin reductase type of ferredoxin-NADP+ reductases potx
... a
side-reaction displayed only by the adrenodoxin reductase
type of ferredoxin-NADP
+
reductases
Matteo de Rosa
1
, Andrea Pennati
1
, Vittorio Pandini
1
, Enrico Monzani
2
, Giuliana Zanetti
1
and Alessandro ... of the product of NADP
+
oxidation.
Results and Discussion
NADPO isolation, quantitation, and spectral
characterization
In order to study the kineti...
... For each user action A
t
, the
ASR engine produces a hypothesis
˜
A
t
of what the
user said, drawn from a distribution Pr(
˜
A
t
| A
t
),
which is the ASR confusion model. The user state
U
t
is ... Concretely, the val-
ues of S
t
, U
t
, A
t
and
˜
A
t
are all assumed to belong
to finite sets, and so all the conditional distributions
in our model are mult...
... Ozawa Y, Nakamura T, Kamata N, Yasujima D,
Urushiyama A, Yamakura F, Ohmori D & Imai T
(2005) Thermococcus profundus 2-ketoisovalerate
ferredoxin oxidoreductase, a key enzyme in the archaeal
energy-producing ... donors
for this key reaction [8].
POR is distributed among archaea, bacteria and
anaerobic protozoa, and is a member of the 2-oxoacid
oxidoreductase (OR) family, wh...
... 5¢-CAGCCGCCGTAGAA
GTT
GTAAGTCATCGCAAAG-3¢,5¢-CGCCGCCACCAGGG
AA
CATCCAGTCAATATCTAC-3, and 5¢-GCCGCCAC
CAGGGAA
TTGCCAGTCAATATCTAC-3¢, respectively.
Confirmation of the mutated nucleotides by automated
sequencing was carried ... In the case of the
E315Q mutant, an additional faint band was also
seen at an M
r
of 43 000. This band appeared as a
degradation product during freezing...
... EcoRI
tr-SpsB5 TA
CATATGCACCATCACCATCACCATATTGTTACACCATATA NdeI
pIsaA5 TA
CCATGGCACATCACCATCACCATCACAAAAAGACAATTATGGC NcoI
IsaA3Myc TA
GAATTCTTACAGATCCTCCTCTGAGATGAGCTTCTGCTCGAATCCCCAAGCACCTAAACC EcoRI
Rao ... sequence is shown in bold.
Oligonucleotide Sequence (5¢ -to3 ¢) Restriction site
fl-SpsB5 TA
CATATGCACCATCACCATCACCATAAAAAAGAATTATTGGAATGGATTATTTC NdeI
fl-SpsB3 TA
GAATTCTTAATTTTTAGTATT...
... tetrahydrofolate pathway, important in
one carbon-metabolism [34]. The data on aminotriazole
indicate that at least in the rat the catalase pathway is of no
paramount importance, and suggest that the ... had little effect on the conversion of
14
C-formate to
CO
2
(but decreased the rates of a -oxidation by 90%). In rat
formate is metabolized by two pathways: th...
... changing the target of the physiological
attack [138]. The acidic component of vipoxin is a nat-
ural inhibitor of the basic and catalytically active
PLA
2
. In the absence of the PLA
2
-like ... non-enzy-
matic subunit has a heavy chain (consisting of A1 and
A2 domains) and a light chain (consisting of A3 , C1
and C2 domains) that are held together by non...
... observed a change in the dis-
tance from atom O28 (at the C3 atom of the pyranose
ring) of this non-reducing sugar to the catalytic aspartic
acid (Asp) responsible for proper orientation of the
acetyl ... moved by more
than 0.3 nm.
Most (83.5%) of the amino acid residues are plotted
in the favourable regions of the Ramachandran plot.
The deviation of geomet...
... Yoshikawa T, Marat D, Doi C, Makino
T, Fukuzawa K, Tsuburaya A, Satoh S, Ito T & Mits-
use S (1998) Insulin resistance in cancer patients is
associated with enhanced tumor necrosis factor-alpha
expression ... example, in intact mitochondria and with suffi-
cient availability of oxygen the rate of oxidative phos-
phorylation is determined by the ATP ⁄ ADP ratio, not
by the...
... conducts
the deamidation reaction. However, the enzymatic
characteristics of PMT have not been analyzed as a
result of the lack of an easily-administered assay to
detect activity of the toxin.
In the present ... shown that G
i2
was activated by the deamidation of Gln
205
to Glu by
PMT from a cell-based assay and MS [15,16]. Ga
q
was
also considered to be...