Báo cáo khoa học: Novel a-conotoxins from Conus spurius and the a-conotoxin EI share high-afﬁnity potentiation and low-afﬁnity inhibition of nicotinic acetylcholine receptors doc

Tài liệu Báo cáo khoa học: Multifunctional host defense peptides: Antimicrobial peptides, the small yet big players in innate and adaptive immunity docx

... heterogeneousgroup of peptides with respect to their primary and secondary structures, their antimicrobial potentials,their effects on host cells, and the regulation of theirexpression. Most ... on their capacity to induce the production of proinﬂammatory cytokines, to pro-mote the recruitment of dendritic cells and monocytesto the site of injury, and to enhance phagocytosis and the ... peptide and of other unusual iso-forms of the peptide. The current thinking is that, atleast partially, the chronic inﬂammation results from the increased chemotactic and angiogenic activity of the...

Báo cáo khoa học: Liver receptor homolog-1 localization in the nuclear body is regulated by sumoylation and cAMP signaling in rat granulosa cells docx

... familyproteins, have been described to promote the transfer of SUMO from E2 to target proteins in vivo. The attachment of SUMO may result in diverse effects on the substrate protein, including ... substrates, and modulate the function of several transcription factors [31].LRH-1 is a target protein of SUMO modiﬁcation[32,33]. The hinge region lysine, residue 289 in the mouse and 224 in the human ... (Fig. 7A). The mRNA levels of Cpy11a1 were 14-, 50- and 263-fold higher after 3, 12 and 24 h, respectively.To determine the effect of cAMP on sumoylationpathway, the expression of the genes...

Báo cáo khoa học: Novel a-conotoxins from Conus spurius and the a-conotoxin EI share high-afﬁnity potentiation and low-afﬁnity inhibition of nicotinic acetylcholine receptors doc

... determination and cloning of the cDNAencoding two novel peptides, SrIA and SrIB, found in the venom of Conus spurius. The pattern and the spa-cing of their cysteines indicate that they belong to the a4 ... aA families.On the basis of the number of residues between the sec-ond and third cysteines and on the spacing between the third and fourth cysteines in the mature a-conotoxins, these peptides ... jI-conotoxins, and the A super-family, containing a-conotoxins, aA-conotoxins and jA-conotoxins [1].Competitive antagonists of the nicotinic acetylcholine receptors (nAChRs) belong to the a and aA families.On...

Báo cáo khoa học: Novel c-carboxyglutamic acid-containing peptides from the venom of Conus textile docx

... Gla(3)–TxVI:conﬁrmation of the presence of BrTrp. Product ionmass spectrum of the doubly charged ion at m ⁄ z ¼660.18. The isotopic distribution of the b2ion (inset)indicates the presence of bromine. The MS ... studies utilizing the Conus c-carboxylase and synthetic peptide sub-strates localized the c-CRS of Gla(1)–TxVI to the region )14 to )1 of the polypeptide precursor: the Kmwas reduced from 1.8 mm ... to the N-terminal signal peptide [7,14–17]. The propeptide mediates binding of the substrateto the carboxylase and also activates the enzyme. The discovery of c-carboxylated conotoxins and, more...

Báo cáo khoa học: Novel cathelicidin-derived antimicrobial peptides from Equus asinus docx

... clinicalapplications of cathelicidins is their haemolysation of mammalian cells. However, the good thing is that the dose of cathelicidin resulting in haemolysis is oftenmuch higher than the MIC. The haemolytic ... Among them, the lung cDNAlibrary was of the best quality, from which some posi-tive clones containing an insert of 555 bp were identi-ﬁed and isolated. The nucleotide sequence of cDNA (from the ... facilitate the treatment of infectious diseases, in addition to offering more novel therapeutic agents to stop the continued emergence of antibiotic resistance. The exact antimicrobialmechanism of cathelicidin...

Báo cáo khoa học: Novel b-1,3-, 1,6-oligoglucan elicitor from Alternaria alternata 102 for defense responses in tobacco docx

... If so, the function of these b-glucan oligomers for the fungus might be verydifferent from that of the known cell-wall glucans.Clarifying the origin of these oligosaccharides and their function ... differentdefense pathways: acidic and basic PR3 encoding chi-tinase enzymes of the PR protein family, PAL and CCoAOMT from the phenylpropanoid pathway, and STC and HMGR from the sesquiterpenoid pathway(Fig. ... FEBSelicitor from 10.2 L of A. alternata 102 culture. The recovery of the elicitor activity was 28% from the starting material, the methanol-insoluble fraction. The speciﬁc activity of the puriﬁed...

Báo cáo khoa học: Novel L-amino acid oxidase with antibacterial activity against methicillin-resistant Staphylococcus aureus isolated from epidermal mucus of the ﬂounder Platichthys stellatus pptx

... this step. The antibacterial activity of gel extracts from the 6 m urea ⁄ PAGE was analyzed using the growth -inhibition plate method, and the molecular mass of the antibacterial protein was confirmed ... molecularmass of the antibacterial protein. Antibacte-rial fractions correspond to the upperdiagram and are indicated by ‘+’. The asterisk indicates the specific band of the antibacterial protein. (C) ... 7.0), thenapplied to a column of HiTrap Phenyl FF high sub(1.6 · 2.5 cm; GE Healthcare) equilibrated with the samebuffer, and the column was then washed with the buffer. The flow rate of the...

Báo cáo khoa học: Novel strategy for protein production using a peptide tag derived from Bacillus thuringiensis Cry4Aa pptx

... matching parts of the sense and antisense strands of the DNA sequence were used. The synthesized fragment was inserted between the BamHI and XhoI sites of pGEX-6P-1. Nucleotide sequences of the recombinant ... [8–13], the structure of the C-terminal nontoxic region has notyet been determined. Therefore, the crystal structure of Cry protoxin is still unknown. The formation of the crystals and their solubility ... space and protects protein from proteo-lytic degradation in the environment. Indeed, Crytoxin accumulates as a protein crystal that can accountfor up to 25% of the dry weight of the cell [2].The...

Tài liệu Báo cáo khoa học: Novel aspects of heat shock factors: DNA recognition, chromatin modulation and gene expression ppt

... sequence, the typ-ical HSE contains at least three nGAAn units [4]. The helical repeat of the DNA (10.5 bp), the length of the unit (5 bp) and the inverted nature of the repeats, posi-tions all of the ... DBD–HSE co-crystals, the protein–protein interface consists of the helix 2 amino-terminus, the turn and the wing[15]. Unlike other winged helix-turn-helix proteins, the wing of the HSF does not ... trimers of HSF1 or asingle trimer of HSF2 [21]. These differences arerelated to differences in the cooperativity of the tri-mers; the wing regions of the HSF1 and HSF2 DBDsH. Sakurai and Y....

Tài liệu Báo cáo khoa học: Novel aggregate formation of a frame-shift mutant protein of tissue-nonspeciﬁc alkaline phosphatase is ascribed to three cysteine residues in the C-terminal extension pdf

... TNSALP (1559delT) being segregated intoCOP II vesicles at the exit site from ER and therefore the mutant protein remains longer in the lumen of the ER and ﬁnally is diverted to the degradation ... oligosaccharide chains of glycoproteins [31]. The involvement of EDEM and ⁄ or SCFFbs2in the degrada-tion of TNSALP mutant proteins is currently beinginvestigated. The aggregate form of TNSALP (1559delT)possesses ... cysteines for serines at position of 506, 521 and 577 of TNSALP (1559delT) provided a clue. The disulﬁde-bonded aggregation almost disappeared in the cell lysate and the culture medium of the cells...

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