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Báo cáo khoa học: Comparative biochemical and functional studies of family I soluble inorganic pyrophosphatases from photosynthetic bacteria potx

Báo cáo khoa học: Comparative biochemical and functional studies of family I soluble inorganic pyrophosphatases from photosynthetic bacteria potx

Báo cáo khoa học: Comparative biochemical and functional studies of family I soluble inorganic pyrophosphatases from photosynthetic bacteria potx

... 3959 Comparative biochemical and functional studies of family I soluble inorganic pyrophosphatases from photosynthetic bacteria Marı´aR.Go´mez-Garcı´a*, Manuel Losada and Aurelio SerranoInstituto ... families: family I, widespread in all types of organism [2], and family II, so far confined to a limited number of bacteria and archaea [3,4]. The families differ in many functional properties; ... (2002)Expression studies of two paralogous ppa genes enco-ding distinct family I pyrophosphatases in marine uni-cellular cyanobacteria reveal inactivation of the typicalcyanobacterial gene. Biochem Biophys...
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Tài liệu Báo cáo khoa học: Molecular modeling and functional characterization of the monomeric primase–polymerase domain from the Sulfolobus solfataricus plasmid pIT3 doc

Tài liệu Báo cáo khoa học: Molecular modeling and functional characterization of the monomeric primase–polymerase domain from the Sulfolobus solfataricus plasmid pIT3 doc

... Naples, Italy3 Institute of Biochemistry, University of Bayreuth, Germany4 Istituto di Biostrutture e Bioimmagini, CNR, Naples, Italy5 Istituto di Biochimica delle Proteine, CNR, Naples, ItalyIn ... Mose´Rossi1,5 and Simonetta Bartolucci11 Dipartimento di Biologia Strutturale e Funzionale, Universita`degli Studi di Napoli Federico II, Naples, Italy2 Istituto di Chimica Biomolecolare, CNR, Pozzuoli, ... continuous linkbetween primase and polymerase activities and the ori-ginal core replicon of primordial cells. In light of thissuggestion, it seems plausible that prim–pol proteins areevolutionary...
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Báo cáo khoa học: Identification and functional expression of a second human b-galactoside a2,6-sialyltransferase, ST6Gal II docx

Báo cáo khoa học: Identification and functional expression of a second human b-galactoside a2,6-sialyltransferase, ST6Gal II docx

... hST6Gal II and mST6Gal II. The two vertical dots indicate identical amino acid residues and single dots indicate similar amino-acid residues. The underlined amino acid residues indicate the sialylmotifs ... ST6Gal II (Eur. J. Biochem. 270) 953rST3Gal III gene was excised with BamHI and HindIII and inserted at the BglII–HindIII site of the p119 transfer vectorgiving the p119-PS ST3Gal III construct. ... III, was precipitated withethanol and air dried, dissolved in water and then digestedwith specific sialidase (sialic acid linkage analysis kit, GlycoInc., USA): NANase I (specific for a2,3-linked...
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Báo cáo khoa học: Identification and functional characterization of a novel barnacle cement protein pptx

Báo cáo khoa học: Identification and functional characterization of a novel barnacle cement protein pptx

... compilation ª 2007 FEBSstiff and tough, and protection from microbial degra-dation. This multifunctionality, together with theinsoluble ⁄ sticky and complex nature of the adhesive,have hindered ... 54% identity and 65% similarity; Mrcp-19k versus Bicp-19k, 51%identity and 68% similarity; and Balcp-19k versusBicp-19k, 61% identity and 75% similarity. All cp-19kscontained two Cys residues, ... and representing 66.4% of allresidues. This ratio is significantly higher than thatdeduced from the standard amino acid composition[13]. The sequence identity and similarity (Fig. 2) wereas...
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Báo cáo khoa học: Molecular cloning and functional expression of the human sodium channel b1B subunit, a novel splicing variant of the b1 subunit potx

Báo cáo khoa học: Molecular cloning and functional expression of the human sodium channel b1B subunit, a novel splicing variant of the b1 subunit potx

... b1Bsubunit. The 5¢ and 3¢ untranslatedregions are indicated by solid thin lines (b1)andshadedthinlines(b1B), and the unidenti-fied 3¢ untranslated region of b1Bis indicatedusing the thin interrupted ... of the primary antibody withnonimmune serum, (b) omission of the primary antibodywith the antibody dilution buffer (Zymed LaboratoriesInc., San Francisco, CA, USA), and (c) preincubationwith ... splicing pattern. Functional studies indicate that the human b1Bsubunit performs aphysiological function distinct from that of the human b1subunit when it is coexpressed with NaV1.2 in...
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Báo cáo khoa học: Quaternary structure and functional properties of Penaeus monodon hemocyanin docx

Báo cáo khoa học: Quaternary structure and functional properties of Penaeus monodon hemocyanin docx

... organization of the oligomers originates from hexameric building blocks. Most of the interestin structure–function studies of Hcs is now focused onthe importance of the oligomeric organization ... repulsiveelectrostatic interaction present in Panulirus isabsent in Penaeus. Interestingly, all crustacean Hc,with the exception of Can. magister and Callinectessapidus, exhibit, in this position, repulsive interactions;Cancer ... oxygen-binding properties. The ulti-mate aim is a precise definition of the allosteric unitresponsible for cooperativity and of the possible role of heterogeneity at subunit level in the modulation...
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Báo cáo khoa học: Purification and functional characterization of human 11b hydroxylase expressed in Escherichia coli doc

Báo cáo khoa học: Purification and functional characterization of human 11b hydroxylase expressed in Escherichia coli doc

... purification of functional hCYP11B1 expressedin E. coli. This will open new possibilities for analyzingthis very important cytochrome P450 in vitro, includingthe detailed investigation of the ... beevaluated using the Michaelis–Menten equation(Fig. 7). Combined with the data obtained from Bia-core experiments, which indicated the possibility of more than one complex formation, and consideringthat ... electrondonors. Taking this into account, this study opens newperspectives for the investigation of the structure and functions of this physiologically important protein.Results and DiscussionPreviously,...
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Báo cáo khoa học: Identification and functional characterization of an aggregation domain in long myosin light chain kinase ppt

Báo cáo khoa học: Identification and functional characterization of an aggregation domain in long myosin light chain kinase ppt

... Animal Research Center, Nanjing University, China2 School of Life Science, Nanjing Normal University, China3 National Institute of Biological Science, Beijing, ChinaMyosin light chain kinases ... transgenic mice exhib-ited similar expression patterns of recombinant 4Ig.Figure 3A shows a typical expression pattern in differ-ent lines, in which the level of expression of 4Ig variedboth within ... theaggregation domain within the 4Ig region in our subse-quent experiments.Overexpression of the 4Ig fragment elicitedprotein aggregation in vitroTo determine the aggregation ability of the 4Ig frag-ment,...
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Báo cáo khóa học: Purification and functional characterization of insecticidal sphingomyelinase C produced by Bacillus cereus ppt

Báo cáo khóa học: Purification and functional characterization of insecticidal sphingomyelinase C produced by Bacillus cereus ppt

... Purification and functional characterization of insecticidalsphingomyelinase C produced byBacillus cereusHisashi Nishiwaki, Katsuhiko Ito, Katsuhiko Otsuki, Hiroyuki Yamamoto, Koichiro Komai and ... theinsecticidal activity, suggesting that the rapid insect toxicity of sphingomyelinase C results from its phospholipid-degrading activity.Keywords: antlion; Bacillus cereus; insecticidal activity;Myrmeleon ... for its insecticidal activity against the cockroachesby injection.Identification of B. cereusTo identify the bacterial species producing insecticidaltoxins, its 16S rRNA gene was amplified...
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Báo cáo khoa học: Molecular cloning and functional expression of a gene encoding an antiarrhythmia peptide derived from the scorpion toxin pptx

Báo cáo khoa học: Molecular cloning and functional expression of a gene encoding an antiarrhythmia peptide derived from the scorpion toxin pptx

... sodium channels with higher affinity than theventricular myocyte sodium channels. Unlike Ts À c and CnII-10 (Ts À cis from Brazilian scorpion Tityus serrula-tus; CnII-10 is from Mexican scorpion ... D600¼ 0.6,induction was initiated by the addition of 1.0 mMisopropylthio-b-D-galactoside (IPTG). Cells were harvested 4 h afteraddition of IPTG by centrifugation and resuspension in1.0 mL ... containing the recombinantBmKIM protein were then lyophilized.Amino acid composition analysis and N-terminalsequencingAmino acid analysis was carried out essentially as describedby Liu &...
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