... Ohkura, M., Furukawa, K ., F ujimori, H., Kuruma, A. ,Kawano, S., Hiraoka, M., Kuniyasu, A. , Nakayama, H. &Ohizumi, Y. (1998) Dual regulation of the skeletal muscle rya-nodine receptor by ... (Temecula, CA, USA).Protran nitrocellulose membranes we re from Schleicherand Schuell (Dasse l, Germany). All other chemicals usedwere of analytical grade and purchased from SigmaChemical Company.Preparation ... Placid, NY , USA; mAb C464.6 t o the a 1-subunit of the Na+/K+ATPase and m Ab VIA41to a- dystroglycan).Peroxidase-conjugated secondary antibodies were obtained from Chemicon International...