... important portion of the fusion protein wasdegraded by the host proteases. On the other hand,attempts to produce a His-tagged version of the Rv1771 protein by using the pRSETc or the GatewaypDEST17 ... acceptor. In contrast to the animal and plant l-gulono-1,4-lactone oxidoreductases, the myco-bacterial enzyme is specific for l-gulono-1,4-lactone and has no activity towards l-galactono-1,4-lactone(Table ... determined for: (A) the L-gulono-1,4-lactone substrate in the presence of 121 lM cytochrome c; and (B) the cytochrome c substrate in the presence of 50 mML-gulono-1,4-lactone. Double-reciprocal...