... Enzymes that hydrolyze adenine nucleotides of patients
with hypercholesterolemia and inflammatory processes
Marta Medeiros Frescura Duarte
1
,Va
ˆ
nia ... understand their
active role in this process and the importance of
such hydrolysis. In line with this, literature data indi-
cate that adenine nucleotides and adenosine are
important modulators of ......
... detected
as a 40-kDa band within the TOY2 and TOY3
strains (Fig. 2A).
In vitro activities of UGD and UXS
We next examined the activity of the hybrid enzymes
from cytoplasmic fractions of the recombinant ... we expressed the AtUGD1
and AtUXS3 genes, which encode UGD and UXS of
A. thaliana, with the hope of producing UDP-GlcA
and UDP-Xyl in yeast. We found that introdu...
... TnC(1–91) and TnI(98–182) form a regula-
tory subunit, and that the first half of the C termi-
nus of TnI is important for this binding.
Structural information of the interaction of TnC
and TnI ... results
are similar with the results observed for the binary
complexes formed by TnC(1–91) and TnI(1–147), and
TnC and TnI [16] and agree with the hypothesis that
the e...
... PAGE
12.5%) of WT and the M
1
,M
2
and M
3
variants of
anSMEbt.
Fig. S4. UV-visible absorption spectra of oxidized and
dithionite-reduced as purified and reconstituted forms
of WT and M1 mutant ... (A) and MALDI-TOF MS (B) analysis of the peptide maturation catalyzed by WT anSMEbt and by M
1
,M
2
and M
3
mutants of
anSMEbt. The WT and mutant forms of anSMEbt (ea...
... of ISP into an immature bc
1
complex and that the association of the Qcr9p subunit
with the bc
1
complex precedes the binding of ISP. In
fact, the direct absence of ISP (DISP), or the block of
its ... new
findings open up several avenues of investigation and
illustrate that a significant amount of work is still neces-
sary for a complete understanding of the assembly
p...
... oxidative
activity with glycerol and NADP. Also, with other C4
and C5 polyols no activity with NADP as a cofactor
was observed. We tested erythritol, ribitol, xylitol and
dl-arabinitol at a concentration of ... electro-
phoresis, and enzyme activities with NADP and gly-
cerol as substrates were visualized by Zymogram
staining; that is, only enzymes that had activity...
... up-regulation of
the expression and enzymatic activity of both AGAT
and GAMT. The specific activity of AGAT in sarcoma
tissue reached almost 50% of that observed in the
kidney and it was also observed that ... formation of polyamines and methionine.
An investigation of the level of different intermediate
metabolites and the expression of different enzymes in
the...
... binding of Man-ol1P and
NAD
+
by AfM1PDH is not ordered, and that there is
also randomness in the binding of substrate and coen-
zyme in each direction of the reaction catalyzed by
AfM2DH. By way of ... also reported random binding
of NAD
+
and Man-ol1P [29]. It is reasonable to
assume that random binding of reactants by
AfM1PDH and AfM2DH occurs in rapid equilibrium...
... enzymatic
reactions; synthesis and modification of polymers.
Introduction
The use of enzymes in organic media (with low water
content) has been one of the most exciting facets of
enzymology in recent ... precipitated with and rinsed with propanol [29],
enzymes obtained after three-phase partitioning [30], cross-
linked enzyme crystals [22], crosslinked enzyme aggregates
[3...
... was
recovered and mixed with an equal volume of SDS buffer
(0.2 m Tris, pH 7.5, 0.3 m NaCl, 25 mm EDTA, 2% SDS)
and twice extracted with phenol ⁄ chloroform, once extracted
with chloroform, and precipitated ... the fate of transcripts with
which it becomes associated.
Materials and methods
Materials
Enzymes were purchased from Invitrogen (Carlsbad, CA,
USA) and Roche (...