... 4097–4110 ª 2008 The Authors Journal compilation ª 2008 FEBS
Ligand binding and antigenic properties of a human
neonatal Fc receptor with mutation of two unpaired
cysteine residues
Jan T. Andersen
1
, ... human HC variants.
Table S3. Numbers of cysteine residues of FcRn HCs
across species
a
.
Table S4. Numbers of cysteine residues of non...
... HPLC analysis of bound nucleotides.
GTP hydrolysis assay and steady-state kinetics
The initial velocity of GTP hydrolysis was measured by a
radioactive assay. It was started by the addition of ... presence of [
32
P]-ATP as described in the methods and then
autoradiographed. (B) In-gel kinase assays show that commercial casein kinase II (left panel, right lane) gave bands compar...
... Xiao G, Grassi J et al. (2006) Prion pro-
teins with insertion mutations have altered N-terminal
conformation and increased ligand binding activity and
are more susceptible to oxidative attack. ... of fatal neurodegenerative
disease in humans and animals. It is believed that all
prion diseases are caused by the conversion of a nor-
mal cellular prion protein (PrP
C
) to a...
... interval of L was calcu-
lated from best-fit values and the standard errors of l
T
, l
R
,
and L according to the law of error propagation [64].
Statistical analysis
Unless otherwise stated, data are ... F-statistics predicted the increase in the vari-
ance (or the SSR) of a fit that is associated with one
standard deviation of the parameter.
Acknowledgements
The technical ass...
... shift assay
A double-stranded 40-mer DNA probe was prepared by
annealing the oligonucleotide 5¢-AGTCTAGACTGCAG
TTGAGTCCTTGCTAGGACGGATCCCT-3¢ and its com-
plementary strand. A 20-mer dsDNA probe was ... 1146–1157.
59 Sasaki N, Kuroiwa H, Nishitani C, Takano H,
Higashiyama T, Kobayashi T, Shirai Y, Sakai A,
Kawano S, Murakami-Murofushi K et al. (2003) Glom
is a novel mitochondrial DNA...
... 5¢-GAGAAACAAGTGATACGTTGGA
GA-3¢ and 5¢-TTTCCTGAATGCAGATGACTCTAT-3¢
from AtSig2 and 5¢-GTAGAGATGAACTGGTGAA
AAGCA-3¢ and 5¢-AAAACTTGTAACCCCTTGTGT
GAT-3¢ from AtSig3, were used for PCR-amplification
from total S. alba DNA. ... sequence
tags with sequence similarity to the coding regions of
the AtSig2andAtSig3 genes from Arabidopsis thaliana
(GenBank accession numbers AC003981 and N970...
... exogenous aquacob(III)alamin
to cob(II)alamin [Yamada K, Gravel RA, TorayaT & Matthews RG
(2006) Proc Natl Acad Sci USA 103, 9476–9481]. Here, we report the
expression and purification of human methionine ... domain of neuronal nitric oxide synthase) to:
(a) stimulate formation of holo-MS from aquacob(III)alamin and the
apo-form of MS; and (b) reactivate the inert cob(II)a...
... influenzae,
Streptococcus pneumoniae, Branhamella catarrhalis and the pathogenic
fungi Aspergillus fumigatus and Candida albicans, in addition to P. aerugin-
osa and S. aureus. A similar antimicrobial ... N-terminal domain and is comparable to that of
human defensins and human lysozyme.
Elafin and trappin-2 are both antimicrobial against
S. aureus and P. aeruginosa [14,15]...
... L, Hamid Q & Elias JA (2004) Acidic
mammalian chitinase in asthmatic Th2 inflammation
and IL-13 pathway activation. Science 304, 1678–
1682.
4 Kasprzewska A (2003) Plant chitinases ) regulation ... 1084–1092.
35 Kawase T, Yokokawa S, Saito A, Fujii T, Nikaidou N,
Miyashita K & Watanabe T (2006) Comparison of
enzymatic and antifungal properties between family 18
and 19 chit...
... bond network
around ArgB263 provide no reasonable explanation of the
PA activity with substrates like PhAc-pAB, PhAc-mAB,
PhAc-oAB vs. phenylacetyl 4-nitroanilide (PhAc-pNA),
NIPAB and iso-NIPAB, ... Distances (A
A
˚
) between polar substrates groups (CO
2
–
and
NO
2
) and the two ArgB263 and ArgA145 residues of penicillin acylase as
a result of AM1 optimization of th...